1. Comportamiento cualitativo de Pseudomonas aisladas de aguas residuales, expuestas a mercurio.
- Author
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Paternina-Mercado, Francisco, Bedoya-Vélez, Jessica, and Ochoa-Agudelo, Susana
- Subjects
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MERCURY compounds , *POISONS , *PSEUDOMONAS , *METALS , *SOIL moisture , *AQUATIC microbiology , *HEAVY metals , *MERCURY poisoning , *MERCURY vapor - Abstract
Mercury is one of the metals used in mining, and when it is disposed of in the soil or water, its distribution and toxic exposure are magnified. The use of microorganisms to remove metals in water provides some opportunities to explore their impact. The objective of this research is to explore the ability to tolerate mercury, of isolates of Pseudomonas spp. gender, recovered from sewage. Water from the Ciénega de Ayapel (Córdoba) and the Cauca River, in Caucasia (Antioquia), were taken and analyzed microbiologically: spread on the Cetrimide agar and biochemical identification by Vitek (bioMérieux). The Pseudomonas spp. isolates were exposed to mercuric sulfate concentrations supplementing BHI medium, using the tube technique (up to 500 mg/L) and microplate (up to 75 mg/L), to identify the tolerance to the metal. As a result, we obtained six Pseudomonas spp. isolates with the capacity to grow in concentrations between 75 and 400 mg/L of mercuric sulfate, showing viability, precipitation, pigmentation, and fluorescence in the medium as protection mechanisms against the metal. Pseudomonas species have been mentioned in several experiments related to the removal of mercury and derived compounds. In conclusion, it was determined that native isolates of Pseudomonas with the ability to tolerate different concentrations of mercury could be used in processes that accompany the removal of this metal, going deeper into the interaction mechanism with the metal so that it is applied in future leachates obtained from mining before being disposed of in the river. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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