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The establishment of a genetic transformation program requires a selection in vitro system of transformed tissues. In the Glycine genus exists few references that employ the Glufosinate-ammoniun that selective agent in transformed somatic embryos. For this reason this work had as objective to determine the minimum lethalconcentration of the herbicide Glufosinate-ammoniun in somatic embryos of Cuban soybean cultivar INCASoy-27, to use as selective agent in genetic transformation programs. The soybean somatic embryos were placed on multiplication medium that contained 2,4-dichlorophenoxyacetic acid 20 mg L-1 with different concentrations of Glufosinate-ammonium (1,0; 2,0; 3,0; 4,0; 5,0; 6,0 mg L-1). All concentrations of selective agent caused necrosis in not transformed soybean somatic embryos. The increments of selective agent concentration and culture time increased the necrosis of somatic embryos. Finally was determined the minimum lethal concentration of 6,0 mg L-1 Glufosinate-ammoniun in semisolid medium, that selective agent in the genetic transformation of somatic embryos of soybean cultivar INCASoy-27. [ABSTRACT FROM AUTHOR]

A fast and efficient methodology for transforming internodal segments in stems of potato (Solanum tuberosum L.) Désirée cultivar, with Agrobacterium tumefaciens and by using phosphinothricin (PPT, gluphosinate ammonium) as selection agent, was developed. Shoots obtained with 68% transformation efficiency were induced 4 to 5 weeks later in the presence of phosphinothricin at 2 mg/L. The chlorophenol red assay was successfully employed in segments of leaves from transformed plants, demonstrating the expression of the bar gene, and its presence was confirmed in the genome of explants by polymerase chain reaction. Moreover, increased resistance of transgenic clones at phosphinothricin concentrations up to 500 mg/L was observed, also showing a normal phenotypic development. These transgenic plants were transplanted into greenhouses where they also showed to be resistant to the Finale® herbicide (Bayer CropScience) at a concentration 2.5 L/ha, growing green and vigorously as did not the non-transgenic control plants that died 7 days after treatment. [ABSTRACT FROM AUTHOR]