Search

Patients who are in the intensive care unit have an accentuated hypercatabolic state in addition to a prolonged resting state, so it is important that the medical effort is aimed at improving all aspects that are affected by the root of the hospital stay. Multidisciplinary therapy is one of the goals that should be achieved in all units so that doctors and physiotherapists are involved early in the rehabilitation of severe patients. The early implementation of the use of the stabilized table in the ICU tries to improve the changes in the different systems of the organism, being adjuvant in conventional medical therapy, with results demonstrated in the improvement of patients in various published studies. [ABSTRACT FROM AUTHOR]

Copyright of Cogitare Enfermagem is the property of Cogitare Enfermagem and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Plant lipases are highly versatile biocatalysts due to their chemo-selectivity, enantio-selectivity, and region-selectivity. The purpose was to obtain a recyclable biocatalyst from Pachira speciosa seed lipases, applicable to lipid bioconversion. A partially purified lipase was obtained from P. speciosa seed extracts, by gel filtration chromatography on Sephadex G-100; the specific lipase activity (LAS) was determined by free fatty acid titration. A Box-Behnken response surface design was applied to establish conditions that maximize lipase immobilization to three supports: chitosan beads (Ch), calcium alginate beads coated with chitosan (Alg- Ch), and chitosan-Fe(OH)3 magnetic beads (Ch-Fe). The highest LAS of the free enzyme was 8,23 ± 0,23 nKat/mg of protein at 40 °C and pH 9. The immobilization percentage and LAs (nKat/mg of beads) of each biocatalyst was EQ = 90,6 % and 3,74 ± 0,3 nKat/mg; Alg-Q = 88,5 % and 3,62 ± 0,1 nKat/mg; EQ-Fe = 76,4 % and 2,88 ± 0,1 nKat/mg. The most stable biocatalyst was the lipase immobilized in Ch, with 85 % retention of LAS until the third catalytic cycle. Future studies will be focused on establishing the kinetic parameters of the biocatalyst. [ABSTRACT FROM AUTHOR]

Mechanical restraint is a coercive procedure in psychiatry, which despite being permitted in Spain, raises significant ethical conflicts. Several studies argue that non-clinical factors – such as professionals’ experiences and contextual influences – may play a more important role than clinical factors (diagnosis or symptoms) in determining how these measures are employed. The aim of this study is to understand how the experiences of mental health professionals in training relate to the use of mechanical restraints in Madrid’s mental health network. Qualitative phenomenological research was conducted through focus groups in 2017. Interviews were transcribed for discussion and thematic analysis with Atlas.ti. Descriptive results suggest that these measures generate emotional distress and conflict with their role as caregivers. Our findings shed light on different factors related to their experiences and contexts that are important in understanding the use of mechanical restraint, as well as the contradictions of care in clinical practice. [ABSTRACT FROM AUTHOR]

Enzymatic immobilization can be used in a wide range of processes. During the last few years, varieties of techniques for immobilization have been implemented, increasing its operational efficiency and its higher use. Nowadays, immobilized enzymes are preferred over free enzymes, since their availability and yields per work cycles are superior, hence, operating costs happen to be reduced in industrial sectors. The applications are mainly focused on sugar industry, biotechnology, bioremediation of waters and soils, among others. Immobilized enzymes have also demonstrated significant application in drug metabolism, biodiesel and antibiotic production, and food industry. The widespread use of immobilized enzymes is largely due to the fact that they are cheaper, more environmentally friendly, and much easier to use compared to equivalent technologies. This study is a review of the extensive variety of existing papers on the different supports for the immobilization of enzymes, their main characteristics and uses, as well as the immobilization processes that can be affected by the type and characteristics of the support materials. [ABSTRACT FROM AUTHOR]

Introduction: Immobilization with plaster cast in lower limb is a frequent procedure for children in the emergency services, which is not without risks related to immobility and pressure, on these factors nurses have to adopt preventive care. Objective: To verify the efficacy of the polymeric heel patch in the prevention of iatrogenic ulcers in children immobilized with a plaster splint in the lower limb. Material and method: Preventive randomized clinical trial on pediatric patients aged 0 to 14 years who require immobilization with a lower limb cast cast. Simple blind non-probabilistic consecutive sampling was performed. Descriptive statistical analysis of the variables and χ²test with the application variables of the patch and the appearance of ulcers. Results: A total sample of n = 74 patients was obtained. Ulcer was observed in 21.7% of patients, whole skin (48.2%) and erythema that pales 30.1%. The χ² test was applied for the application of polymer patch and ulcer appearance variables, obtaining χ² = 0.135 with p = 0.713, and therefore, there is no statistical significance between these two variables. Conclusions: There is no direct relationship between the prevention of pressure ulcers in children immobilized with plaster splint and the application of polymeric protective patch. It is very important that nurses offer a quality health education in the care and maintenance of the splint to avoid complications. [ABSTRACT FROM AUTHOR]

Copyright of Ciberrevista Enfermeriadeurgencias.com is the property of Sociedad Espanola de Enfermeria de Urgencias y Emergencias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Colombiana de Investigaciones Agroindustriales is the property of Revista Colombiana de Investigaciones Agroindustriales del Centro Agropecuario Sena Buga and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the world the production of whey is increasing and its insufficient use generates environmental and economic impacts that are reflected in the deterioration of the environment and the loss of economic resources for the communities. The aim of the present review was to consult in the scientific and technical literature the different uses of whey from proteins and lactose. It was identified that whey is still commonly used to obtain powdered whey and concentrates and protein isolates, among other products. The processing of whey generates waste such as lactose, which represents a serious environmental and economic problem due to the contamination of water sources and the high cost of its treatment (labor and consumables). In this sense, the production process of galactooligosaccharides (prebiotic compounds of high interest) can be an alternative of investment and technological development for the dairy sector of Colombia that searches for new ways to valorize the waste and diminish the environmental impact of these processes. [ABSTRACT FROM AUTHOR]

Copyright of Revista de la Sociedad Química del Perú is the property of Sociedad Quimica del Peru and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The grade II ankle sprain it is one of the most frequent pathologies in the musculoskeletal system and one of the principal motives of laboral inability. Despite the fact this pathology is one of the most common reasons for ER visiting there is not a standard treatment for it, and exist controversy about the immobilization vs. an early mobilization. Objective: Determine the best treatment for grade II ankle sprains, offering a satisfactory result with a minor time of labor inability. Material and methods: Single-blind randomized controlled clinical trial with two groups immobilization versus functional bandage, both groups with VAS evaluation at the beginning and at the end of treatment, same AINES management and rehabilitation, 30 days after the injury we evaluate the functional outcome with Karlsson score. Results: At the beginning all patients report pain over 70 (moderate-severe) without difference in the average of both groups (immobilization 86.3 ± 9.6 versus functional bandage treatment of 86.1 ± 8.5; p = 0.95). Both groups present less pain at the end of the treatment, the bandage group present lower values, (Immobilization average 55.2 ± 15.5 versus functional bandage treatment 40.3 ± 12.8, p ≤ 0.001). In the immobilization group 24 (88.9%) at the end of the treatment had moderate pain and 3 (11.1%) light pain against the functional treatment group where 17 (73.1%) had moderate pain and 7 (26.9%) light (p = 0.15). Conclusion: Functional bandage is an excellent treatment option for grade II ankle sprain, offering good results with a lower time of inability and cost. [ABSTRACT FROM AUTHOR]

Removal of inorganic nutrients from wastewaters by microalgae is an efficient and productive method, which provides oxygenation to water bodies and useful biomass for several industries. The aim of this investigation was to determinate the nitrate (N-NO3-) and phosphate (PO43-) removal capacity by free and immobilized cultures of native strains of Chlorella sp. (Chlorelaceae) and Chlamydomonas sp. (Chlamydomonadaceae) in municipal wastewaters. Removal experiments were performed in sterile wastewater effluents from the same plant during 10 days. Microalgae were immobilized in 4 % sodium alginate discs. Free Chlamydomonas sp. reported the maximum N-NO3- removal percentage (75.08 %) while free Chlorella sp. showed the highest PO43- removal (83.69 %). Most of the cultures removed more than 50 % and 70 % of N-NO3- and PO43-, respectively, during the first day. Immobilization affected negatively the nutrient removal, however, these values were higher or within high ranges of removal reported in previous studies. The results showed that both strains in free or immobilized cultures have the potential to be used for removal of inorganic nutrients in municipal wastewaters, especially for PO43- removal. [ABSTRACT FROM AUTHOR]

Copyright of Ciberrevista Enfermeriadeurgencias.com is the property of Sociedad Espanola de Enfermeria de Urgencias y Emergencias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Médica Clínica Las Condes is the property of Editorial Sanchez y Barcelo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of DYNA - Ingeniería e Industria is the property of Publicaciones Dyna SL and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de la Sociedad Química del Perú is the property of Sociedad Quimica del Peru and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The selection and procurance of the immunoreagents used in the immunoassay and biofunctionalisation transducer strategy, are a key in the piezoelectric immunosensor development for the detection of tuberculosis (TB). Many have reported the use of 38kDa protein antigen (Ag38kDa) from Mycobacterium tuberculosis (Mtb) such as good biomarker of TB disease and compliance with physical and biochemical characteristics to be immobilized by self-assembled monolayers (SAMs), in the gold electrode of piezoelectrics crystals surfaces. A piezoelectric immunosensor developed from purified native antigens of Mtb may be an alternative simple method for detection of Mtb with speed and reusable advantages, contributing to the control and early treatment of disease. In this paper, the purification process of Ag38kDa Mtb from secretory proteins filtered culture (CFP) from Mtb is presented as an immunoreactive with potential application in the detection of Mtb by piezoelectric immunosensors. Functionalized crystals were obtained by using the modified self-assembled monolayers (SAMs) technique, with purified native antigen and CFP. The functionalized surfaces were qualitatively characterized using atomic force microscopy (AFM) in order to validate the immobilization protocol optimal conditions for secretion antigens from Mtb. These modified crystals may be coupled to piezoelectric immunosensor characterization system for detecting of Mtb by a direct competition immunoassay. [ABSTRACT FROM AUTHOR]

Fe3O4, Fe3O4-NH2, Fe3O4@SiO2 y Fe3O4@SiO2-NH2 as magnetic supports were studied for the urease immobilization. The solids were characterized by N2-physical adsorption at 77 K, magnetization curves and FTIR. Activity assays were evaluated by urea decomposition. The magnetic properties of solids allow an easy separation. The immobilized enzyme improved the enzymatic activity compared to the free enzyme. In Fe3O4-NH2 and Fe3O4@SiO2-NH2 was observed allows a greater amount of enzyme coupled. [ABSTRACT FROM AUTHOR]

Copyright of CES Medicina is the property of Universidad CES and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Colombiana de Biotecnología is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Universitas Médica is the property of Pontificia Universidad Javeriana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Advances in the design of a bioreactor in the fats and oils industry have permitted the hydrolysis of triglycerides in mild conditions and improved productivity while avoiding the formation of unwanted byproducts. The present work develops a mathematical model that describes the hydrolytic activity of a tubular reactor with immobilized lipases for the production of glycerol and fatty acids from the oil trade. Runge Kutta's numerical method of high order has been applied, considering that there is no accumulation of the substratum in the surface of the membrane, where the enzyme is. At the same time, different equations based on the kinetic model of Michaelis Mentens and the Ping-Pong bi-bi mechanism were examined. Experimental data in discontinuous systems are the basis for the development of the quantitative mathematical model that was used to simulate the process computationally. The obtained results allow for optimizing both the operative variables and the economic aspects of industrial processes. [ABSTRACT FROM AUTHOR]

Congenital knee dislocation is an infrequent condition with unknown etiology. In some cases it occurs as an isolated condition, while in others it coexists with associated conditions or syndromes. The treatment of congenital knee dislocation is driven by the severity and fl exibility of the deformity. The literature includes from serial casting or the Pavlik harness to quadriceps tendon plasty or femoral osteotomies. We report herein the case of a congenital dislocation treated with serial casting with a good outcome. [ABSTRACT FROM AUTHOR]

Copyright of Actualizaciones en Osteología is the property of Asociacion Argentina de Osteologia y Metabolismo Mineral and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To compare the Robert Jones bandage with the «U» splint in edema control of ankle fractures. Material and methods: A prospective, longitudinal study, open, comparative and randomized made in 32 male patients (72%) and 16 female patients (28%), the average age was 36.9 years. Patients with an ankle fracture within 12 hours of evolution were registered. Both ankles previous to immobilization were measured, dividing the patients in 2 groups. Group 1 were treated with Robert Jones Bandage, and group 2 with «U» splint. The pain previous to immobilization was registered using an analogous visual scale. Twenty-four hours later the circumference and the pain was measured again. Results: Group 1 displayed less edema, mean 17.69 mm, in comparison with group 2, of 8.33 mm (p ≤ 0.36). Pain previous to immobilization in group 1 was a mean of 7 and in group 2 of 5.5 (p ≤ 0.3); 24 hours later, a mean of 2 in both (p ≤ 0.8). Conclusions: There were no significant differences between both immobilization methods. [ABSTRACT FROM AUTHOR]

AIDS epidemic is one of the most serious problems affecting humanity today. Since science, despite all the great efforts made by scientists throughout the world, has not been able to find an effective vaccine to cure this disease, the use of anti-retroviral drugs has given the most successful results in improving life quality of AIDS patients. Hence, the importance of having the means to carry out an early diagnosis of the infection, as well as monitoring its progression with the purpose of having an influence on it. The present research work describes the obtaining of biological components required for the production of two kits used in Cuba for all HIV-AIDS related studies. To achieve this, a protocol was made that included different stages in the purification and immobilization of several molecules. Monoclonal antibody anti p24, HIV-1 p24, and polyclonal human antibodies anti p24 were obtained, with an adequate level of purity and biological activity allowing its use as intermediate products in the above mentioned stages and as reagents comprised in the kits. [ABSTRACT FROM AUTHOR]

A study of the quantity for ethanol and protein production from the yeast Saccharomyces cerevisiae Scer 02, also in the alginate immovilization and free forms, has been done in this work. The results are increased when the yeast is in the immovilization form. A statistical analysis over these results is made. [ABSTRACT FROM AUTHOR]

Se evaluó la inmovilización de cinco lipasas en octil agarosa: cuatro nuevas lipasas obtenidas a partir de microorganismos extremófilos (denominadas 499EST, 513EST, 514LIP y 828LIP) y la de Candida antárctica B (CALB), mediante determinación de la actividad enzimática relativa en la suspensión y en el sobrenadante, utilizando p-Nitrofenilbutirato (pNPB) como sustrato. Los derivados obtenidos fueron probados en diferentes valores de pH y evaluados en cuando a su estabilidad térmica y su actividad catalítica. Se compararon los resultados obtenidos entre las enzimas solubles y las inmovilizadas, frente a CALB soluble e inmovilizada. Se encontró que el proceso de inmovilización de las lipasas 514, 828 y 513 generó un incremento en la actividad catalítica en un factor de 3, 16 y 20 veces respectivamente, contrario a la lipasa patrón (CALB) y a la lipasa 499 que presentó una disminución de casi un 40% de su actividad inicial. Por otra parte, la inmovilización de la lipasa 499 confirió estabilidad térmica e hiperactivación a la enzima tras 24 horas de incubación a 90°C, ligeramente mayor al 150 y 250% de la actividad inicial que presentaron las lipasas 828 y 513, respectivamente. En este mismo estudio la lipasa 514 inmovilizada presentó una actividad 10 veces mayor a la enzima soluble, en las mismas condiciones de incubación. Finalmente, y debido a la inmovilización reversible de las lipasas sobre el soporte de octil-agarosa, estas pudieron ser desorbidas y caracterizadas mediante técnicas electroforéticas, comprobando la purificación de las mismas, asegurando que solo las lipasas se pegaron al soporte hidrofóbico. [ABSTRACT FROM AUTHOR]