1. Pichia pastoris: una plataforma para la producción de proteínas heterólogas.
- Author
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Serrano-Rivero, Yunier, Marrero-Domínguez, Karen, and Fando-Calzada, Rafael
- Subjects
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PICHIA pastoris , *YEAST , *PROTEINS , *PROTEIN expression , *IMMUNOGLOBULINS , *INSULIN , *ERYTHROPOIETIN - Abstract
The yeast Pichia pastoris has been widely used as a host system for heterologous protein production, such as; antibody fragments, recombinant huridina, recombinant human insulin and recombinant human erythropoietin. Its wide application is due to the availability of the promoter of alcohol oxidase enzyme, which is strong and regulatable by methanol and its ability to reach high cell densities in bioreactors cheap culture media. This review aims to provide information about yeast P. pastoris as suitable host system for heterologous protein production. The majority of the P. pastoris strains that are employed in the production of heterologous proteins possess as auxotrophic selection marker the histidinol dehydrogenase gene (his4), which is essential for synthesis of histidine amino acid. As this yeast is capable of using methanol as carbon source and energy, most of its expression vectors are based on promoters of enzymes involved in the methanol metabolism among which is the alcohol oxidase 1 (AOX1). The strain selection, vectors and optimization of culture conditions for a bioprocess with P. pastoris is essential in the obtaining of high levels of the heterologous protein. [ABSTRACT FROM AUTHOR]
- Published
- 2016