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Contextualization: Hydrolases are some of the most used enzymes in different industrial processes and can be obtained from microorganisms such as bacteria and fungi. Filamentous bacteria are a group of microorganisms present in various environments, and many of these species produce extracellular hydrolases catalyzing the decomposition of compounds such as proteins and polysaccharides. The most representative genus is Streptomyces, a bacterial group found in most soils. It synthesizes enzymes of interest and main antibiotics of microbial origin. Knowledge gap: The high soil diversity in the country is an opportunity for the bioprospecting of native microorganisms with enzymatic potential, which can be found in different understudied agroecosystems and their biochemical capacities could be associated with the environmental and nutritional conditions of the sites where they are found. Objective: To verify the presence of filamentous bacteria producers of hydrolytic enzymes with potential industrial application in different soil types and a composting system. Methodology: Samples were taken from a composting system, from the floor of a path in a native forest, from the rhizospheres of a live fence, and hydrangeas, avocado, blackberry, and Cole crops. Filamentous bacteria were isolated based on the colonies with the corresponding morphology. Enzyme activities were determined using the disk diffusion method to quantify the halos of hydrolysis in starch, carboxymethyl cellulose, gelatin, and olive oil mediums. Results and conclusions: 43 isolates of filamentous bacteria were obtained, of which 36, 42, 39, and 30 had amylase, cellulase, gelatinase, and lipase activity, respectively. At least one isolated with some hydrolytic activity was found in all samples. The composting system and the rhizosphere of the live fence were the sites from which more bacterial isolates were obtained with higher activity. It is likely related to characteristics such as humidity and organic matter from the soils from which they were recovered. [ABSTRACT FROM AUTHOR]

Filamentous fungi are organisms frequently used to develop alternatives to solve challenges in different productive representing a competitive opportunity with economic value in the agriculture, food and pharmaceutical, as well as industrial and biofuels sector Continuing previous research at Universidad Francisco de Paula Santander (UFPS), 25 samples from the strain bank were used for molecular characterization and taxonomic identification. DNA from each strain was obtained under the phenol-chloroform procedure, then, using PCR technique and internal transcript spacer (ITS) primer 4 and 5, amplicons were acquired to be sequenced. Results showed taxonomy categorizations in 4 orders Hypocreales, Eurotiales, Pleosporales y Saccharomycetales with specimens from genus like Aspergillus, Curvularia; Purpureocillium, Penicillium y Trichoderma among others. Findings were laid into a cladogram to display their phylogenetic proximity, and connecting their biotechnological potential towards developing bio-materials, pharmaceutical products and bio-catalysts. Thanks to present study and data base review, it was possible to advance into describing the biological capabilities of these fungi towards developing biotechnological products and services with an approach in phytopathology research. [ABSTRACT FROM AUTHOR]