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INTRODUCTION: Ralstonia mannitolilytica is a causative agent of hospital-acquired infections. The species of the Ralstonia genus are difficult to identify and differentiate. Our objective was to identify and typify R. mannitolilytica isolates obtained from blood cultures of pediatric patients. METHODS: We collected 10 isolates that were identified with an automated system, a Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF-MS) system and 16S rRNA gene analysis. The resistance profile was determined and the carbapenemases were typed through PCR and sequencing. The clonal relationship of the isolates was established by Pulsed-field gel electrophoresis (PFGE). RESULTS: The MALDI-TOF-MS system and 16S rRNA gene analysis identified the isolates as R. mannitolilytica; all belonged to a single clone and were resistant to meropenem. The carbapenemases OXA-443 and OXA-444 were detected in all strains. CONCLUSIONS: This is the first report of R. mannitolilytica isolates with clonal origin and harboring the carbapenemases OXA-443 and OXA-444 in Mexican pediatric patients. [ABSTRACT FROM AUTHOR]

Bacterial wilt, caused by Ralstonia solanacearum, is one of the most important bacteria plant diseases affecting crops in the tropical and subtropical regions at a wide range. In Cuba, this disease is under strict quarantine measures; however entrance risk grows every year due to the import of vegetal material coming from areas of high infestation levels. In this work, the use of the polymerase chain reaction (PCR) for the detection and confirmation of R. solanacearum in imported materials, as well as the surveillance of the disease in the national system of plant health are introduced and developed The methodology used made easy the detection of R. solanacearum in cell cultures up to 10¹ UFC/mL and in total DNAs. Nowadays, such methodology has been used in the evaluation of potato seeds imported every year which allows strengthening the prevention and surveillance systems of the bacterial wilt in Cuba [ABSTRACT FROM AUTHOR]

The bacterial wilt, caused by Ralstonia solanacearum, affects various economically important crops. A successful disease management depends on the precise and early diagnostic. The objective of the present work was to optimize the serologic methods to detect R. solanacearum. Antibody polyclonal was obtained for being used in the serological techniques. The sensitivity and specificity of methods were determined. The detection limit was from 104 to 106 CFU/mL detected in plant extracts and tubercles by ultramicroanalytic system, ELISA, DOT-BLOT, latex agglutination and immunofluorescence. When the sample was first incubated in SMSA broth prior testing, the sensitivity of pathogen detection used was as low as 10² CFU/ml. R. solanacearum isolates belonging to biovars 1 and 2 were successfully detected with this technique. No cross-reactions was obtained with other genera and bacteria species studied. The analytical parameters to serology tests were estimated above 90%. [ABSTRACT FROM AUTHOR]