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Copyright of Revista Colombiana de Ciencia Animal - RECIA is the property of Revista Colombiana de Ciencia Animal - RECIA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: The European BIOMED-2 consortium was created to evaluate clonality in lymphoproliferations that are difficult to diagnose. In Colombia, the implementation of these tests began in 2015 at the Instituto Nacional de Cancerología E.S.E., Bogotá. Objectives: To determine the behavior of the rearrangement tests for lymphoid clonality and the difficulties of its implementation in our field through a series of retrospective and consecutive cases of lymphoid proliferation subjected to the BIOMED-2 protocols. Materials and methods: Clinical and histological data and the results of the rearrangement analysis of all cases of lymphoid proliferation subjected to the BIOMED-2 protocols between February 2015 and May 2019 were collected from clinical histories. Results: We recovered 132 samples from which 47 corresponded to reactive lymphoid hyperplasias, 62 to T lymphomas, 19 to B lymphomas, and three to lymphoid neoplasms of unestablished lineage. Only in one case did DNA extraction fail. According to these results, the greatest diagnostic difficulty for the pathologist was the analysis of T lymphoid infiltrates, most of which (44) were skin lesions. Conclusions: Clonality tests can be used in tissues of different quality to help in the diagnosis of lymphoid proliferations that are difficult to classify. It is important to implement and interpret them in an interdisciplinary way considering each case separately. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of BIOAGRO is the property of Revista BIOAGRO and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de Investigación en Salud VIVE is the property of Revista de Investigacion en Salud VIVE and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Hydrolyzates have allowed adding value to foods with a high amount of proteins such as legumes because they improve the bioavailability of their proteins, therefore the purpose of this investigation was to obtain an enzymatic hydrolyzate from andean lupin flour. For which pineapple juice was used as the source of bromelain. Two factors were studied: pH 5.0 and 6.0;and substrate-water ratio 1/10 and 1/20. The prediction equations for the yield and the degree of hydrolysis measured as amino nitrogen percentage were obtained as well as the optimum conditions for the maximum desirability of 87.3 % which were pH 5.0, substrate-water ratio 1/10.3, and 60 min for the hydrolysis time allowed to reach 0.88% of amine nitrogen, from these conditions an scale up for industrial production was performed. The hydrolyzate characterization was carried out by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography, which allowed to conclude that the protein was fractionated in peptides and as a techno-functional property, its solubility improved and the hydrolyzate could be applied as a functional ingredient. chitosan concentration in both strains. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of this research is the protein characterization of three milk beverages that are made from sweet whey with protein contributions from two vegetable matrices (quinoa-based milk drink, soy-based milk drink and fermented milk drink), to The research concentrated the protein by isoelectric precipitation at pH 4.0 values without solubility, and by means of electrophoresis we identified the protein profile of milk drinks, finding bands with molecular weights between 16 and 85 KDa in the fermented milk drink and the milk-based drink. of soy, and 16 to 79 KDa in the quinoa-based milk drink. Gastrointestinal digestibility was established where gastric digestion with the enzyme pepsin was developed, and duodenal digestion with the enzyme pancreatin, after the enzymatic reaction, the electrophoresis technique was applied and it was verified that each of the milk beverages is digestible within the organism. This research seeks to strengthen the development of local agribusiness and above all to demonstrate that the three dairy drinks developed are easily digested and therefore suitable for human consumption and their proteins described are an important contribution to the nutrition of the body. in such a way that the release of peptides, together with their size, hydrophobicity and sequence, constitute essential requirements to convert them into peptides with biological activity. The latter, also considered as bioactive peptides or biopeptides, have recently aroused the interest of researchers because they can favorably influence the functions carried out by the immune, gastrointestinal, nervous and cardiovascular systems, impacting consumer health. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fusarium wilt of banana is a typical vascular wilt disease caused by Fusarium oxysporum f. sp. cubense. The genetic improvement program of the crop needs tools that allow the quick detection of fungal phytopathogen. The objective of this work was to establishment a simple detection protocol of F. oxysporum race 1 in Musa spp. samples by polymerase chain reaction (PCR). The principal related aspects in this protocol are the sampling, fungal in vitro culture, quick DNA extraction method, PCR protocol, electrophoresis separation of PCR products, and general considerations during the work. [ABSTRACT FROM AUTHOR]

Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cucurbitaceae contribute to the diet of the population, as well as to the economy of farmers; but are harmed by phyto-parasites like to Meloidogyne spp. The know about the root-knot nematode is empty to put suitable strategies to management. The objectives of this research were identified species of Meloidogyne genera in cucurbitaceaes and determine their distribution and occurrence in Arequipa, Peru. Roots of Cucurbita maxima, Citrullus lanatus, Cucumis melo and Cucurbita pepo were collected, identifying them by electrophoresis for the enzyme esterase. Meloidogyne spp. is present in 87.1% from places that were evaluated and M. incognita I1 (Rm: 1.00), M. incognita I2 (Rm: 1.02 - 1.07), M. incognita S2 (Rm: 0.87), M. arenaria A1 (Rm: 1.19), M. arenaria A2 (Rm: 1.19 - 1.25), M. hapla H2 (Rm: 0.95) and one specie atypical of Meloidogyne (Rm: 0.87) were identified with occurrences of 17.14%, 42.86%, 2.86%, 17.14%, 2.86%, 11.43% y 5.71% respectively. [ABSTRACT FROM AUTHOR]

The present paper presents an economic and financial evaluation of the process of obtaining the collagen matrices that must be used in the restoration of the tissues of the human body. The objective is to demonstrate the feasibility of investing in such a process, taking into account the indicators established for this. The research methods, techniques and tools applied were: documentary review, non-standardized interview, Pareto chart, synoptic diagram of the Operations and Inspections Process (OPERIN), quality function deployment (QFD), brainstorming, among others. As a main result of the economic-financial evaluation carried out, the definition of a new procedure was enunciated, which was described by the Operation Inspection Diagram (OPERIN) technique and it was also demonstrated that it is feasible to obtain collagen matrices, determining that the term of recovery of the investment is 9 months. [ABSTRACT FROM AUTHOR]

The genetic variability of S. aureus strains isolated from some cases of bovine mastitis was determined. 335 cows from 27 stables were sampled in 10 municipalities in the state of Jalisco. S. aureus strains were identified from milk samples of each mammary gland of each cow, which were grown in blood agar and based on the characteristics of the culture, biochemical tests, and finally their molecular confirmation by PCR. The genetic variation in the strains was identified by pulsed-field electrophoresis technique. The images of the gels were analyzed using the Bionumerics® software. 2.26% of clinical mastitis and 40.45% of subclinical mastitis were diagnosed with the California test. A frequency of appearance of S. aureus of 9.8% of the total sampled glands was recorded. A genetic variation of 14.9% was observed. The 32 strains analyzed were grouped into pulsotypes with 95% or more of genetic similarity, resulting in 12 pulsotypes. It is concluded that there is great diversity in the genetic variability of S. aureus strains from different stables in the state of Jalisco and a great genetic similarity of strains within each stable. [ABSTRACT FROM AUTHOR]

Few are the investigations focused on the biotechnological production and the development of analytical tools about Lentinula edodes, an edible mushroom which has a potential for being used in the development of nutraceutical products. For this reason, in this research, the production of biomass of the mushroom over time by liquid state fermentation (LSF) was studied. Then, the conditions that allow obtaining protein extracts for the application of tools for proteomic analysis were selected. Trichloroacetic acid (TCA) - acetone and TCA - acetone - phenol were the two protein extraction methods which were compared in terms of extraction yield and separation profiles in 1D (SDSPAGE) and 2D (IEF-SDS PAGE) electrophoresis. The highest production of biomass and total protein content was obtained after 10 days of LSF. Protein extraction with TCA - acetone - phenol presented the highest yield, resolution and number of bands in 1D electrophoresis. In 2DE the two methods allowed the extraction of proteins with isoelectric points in pH 3-10 range but, the TCA - acetone - phenol method favored a differential extraction of proteins in the range of 33 to 113 kDa. These results constitute a first application of electrophoretic separation techniques for future proteomic studies. [ABSTRACT FROM AUTHOR]

Copyright of Analecta Veterinaria is the property of Universidad Nacional de La Plata and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A fraction of the circulating fibrinogen contains a variant of the γ chain that is originated by mRNA alternative splicing denominated γ' whose concentration in plasma has been related to an increase in the risk of cardiovascular diseases. Thus, the objective of this work was to design a more efficient γA/γ' fibrinogen purification method in relation to those described in the literature from human plasma. The γA/γ' fibrinogen was purified from the total fibrinogen obtained by precipitation with β-alanine, using separation by fast protein liquid chromatography. Fibrinogen γA/γ', was confirmed by Western blot and its concentration was determined by ELISA. The method showed advantages compared to classical separation methods, for example, smaller amounts of sample could be fractionated quantitatively into pure components in less time (30 min). Therefore, it can be concluded that the technique used for the purification of fibrinogen variants, corresponding to Fg γA/γA and Fg γA/γ', is an efficient separation method that allows purifying the Fg γA/γ' free of main contaminants, as confirmed by immunoelectrophoresis. [ABSTRACT FROM AUTHOR]

In the present study a workflow, that can be applied to different plant species, to obtain a high quality apoplastic extract and for proteomic analysis is described. For that, using carnation roots and stems, some important parameters for the extraction of these proteins were evaluated. The best conditions for the infiltration were provided by using a 0.1 M sodium phosphate buffer pH 6.5 , 50 m M sodium chloride and Pmercaptoethanol 0.1%, with 3 vacuum times at 70 kPa for 20 s and centrifuged at 4°C at 1 , 0 0 0 g for 2 0 min, and then a subsequent protein precipitation and concentration with the Trichloroacetic acid/acetone protocol. These conditions can be used to obtain a good quality extract for 2D electrophoretic analysis o f apoplastic proteins. Strips with pH gradients between 5-8 and 3-10 were used to run isoelectric analysis for extracts obtained from apoplast in roots and stems, respectively. The conditions described can be used to perform studies focused on apoplastic proteins and its role in biological phenomena involving this plant species. [ABSTRACT FROM AUTHOR]

Plant cell cultures are homogenous experimental systems, highly controllable that allow the study of short and large water stress adaptations without the interference of the different tissues and development of plants. An approach to understand these adaptations is through the presence of induced proteins; as a result of changes in genetic expression. This work analyze the response of Fouquieria splendens ssp. breviflora cell cultures exposed to abscisic acid (ABA), through the electrophoretic characterization of quantity and quality of stress induced proteins. There were recorded low molecular weight polypeptides (< 35kDa), common in experiments under ABA 10mM, followed by the association with 20 and 30mM ABA conditions, with a particularly response of cell cultures without the stress agent. [ABSTRACT FROM AUTHOR]

Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To evaluate the results of estimating the functional status of blood-cerebrospinal fluid barrier (B-CSFB) and intrathecal immunoglobulin synthesis (ITS) of IgG for central nervous system by modified commercial techniques designed for other biological fluids. Methods: Disc polyacrylamide gel electrophoresis and quantification of CSF total protein in CSF and serum of 61 patients were conducted at the Institute of Neurology and Neurosurgery (INN) between April and September 2014. The concentration of albumin and IgG was determined by commercial standardized techniques and by modifications of commercial techniques. Albumin ratio and IgG index were calculated and employed to evaluate B-CSFB permeability and ITS, respectively. Results: Total protein and protein electrophoresis patterns were correlated with the quantifications performed by modified commercial techniques. There was no significant difference between validated commercial techniques and modified techniques for quantifications of CSF albumin (Z=0,41; p=0,6791), serum albumin (Z=0,08; p=0,9382), Qalb (Z=0,21; p=0,8361), CSF IgG (Z=1,65; p=0,0995) and IgG index (Z=1,10; p=0,2721). Conclusions: The immunological studies in CSF obtained from modifying commercial techniques designed for other biological fluids are useful to estimate the functionality of B-CSFB and intrathecal immunoglobulin synthesis. The modifications carried out for the turbidimetric technique and for the UMELISA kit should be standardized for the validation of BCSF-B permeability and IgG ITS by the nervous system. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cryptococcus neoformans is a pathogenic yeast that causes meningoencephalitis, specially in AIDS patients. Epidemiological studies of clinical isolates have been encouraged due to the increase in its incidence for infection monitoring and control. Pulsed Field Gel Electrophoresis (PFGE) is the most popular technique for microbial molecular subtyping with epidemiological purpose, because of its versatility and reproducibility, but the analysis of eukaryotic genomes, like C. neoformans, in a single PFGE experiment, does not reveal mutations that do not alter the chromosomes size. That is why the author proposes a two-dimensional PFGE methodology for the C. neoformans genome characterization. The present describes the bioinformatic procedure used to design and verify in silico the efficacy of such methodology. The resolution of the intact chromosomes was achieved in the first dimension (1D) after 9.3 h of electrophoresis in the CHEF minichamber, applying pulses between 30 and 90s. The chromosomal fingerprint was obtained in the second dimension (2D) after restrict the individual bands with Not I or Sfi I and then separate the fragments in the CHEF minichamber applying pulses between 1 and 15 s for 5 h. The proposed methodology reveals the genetic differences in the genome of C. neoformans strains that were invisible in 1D, in 14.3 h of electrophoresis (1D + 2D), in contrast with the 30-40 h that takes a PFGE in regular electrophoretic chambers. Such methodology could be applied for studying the genome of other unicellular eukaryotic species. [ABSTRACT FROM AUTHOR]

The single cell gel electrophoresis assay, better known as Comet Assay (CA), has obtained a privileged position among the tests used to assess the response of cells to genotoxic agents, biotic and abiotic stresses which lead to DNA damage. Different versions of the assay have been developed aimed to widen the spectra of DNA lesions that can be detected. Guidelines have also been provided for its use in ecotoxicological genetics. CA stands out by its demonstrated sensitivity to detect low levels of DNA damage, speediness and straightforwardness for data analysis in single cells, the use small size samples, its flexibility, low cost and applicability to almost any cell population. This set of advantages justifies its wide use for the genotoxicological evaluation in vitro of industrial chemicals, agrochemicals, pharmaceuticals, active principles, as well as for the environmental and human biomonitoring of genotoxicants. The broad variety of organisms where this assay could be applied increases its range of utility and applicability in ecotoxicology. Therefore, this review deals with the use of the CA in the main model organisms (plants, protozoa, small crustaceans, insects, earthworms, mollusks, fish, amphibians and wild mammals) used in ecotoxicology studies. The wide variety of studies using this assay and its many advantages to detect genotoxic effects in organisms of different ecosystems, make it highly suitable for ecotoxicological analysis. [ABSTRACT FROM AUTHOR]

Serratia marcescens is a Gram negative bacterium belonging to the Enterobacteriaceae family, that has become an important opportunistic pathogen associated with a number of life-threatening diseases and nosocomial infections, particularly of urinary and respiratory tracts infections in patients with debilitating illness and outbreaks of sepsis in special care units, in addition, is an important cause of many different illness like meningitis, sepsis, endocarditis and osteomyelitis. Most efforts have been directed at identifying the phenotype of S. marcescesns. However little is known about de outer membrane antigens of this bacterium, and how they is relate to its pathogenicity. Studies examining this question have looked mostly at the antigenic basis serum resistance. [ABSTRACT FROM AUTHOR]

Up to now there is no specific equation to predict the linear DNA migration under Transverse Pulsed Field Gel Electrophoresis (TAFE) minichambers conditions. Consequently, the selection of the experimental electrophoresis conditions for miniTAFE system is currently done by trial and error, which is inefficient because it takes a lot of time and reagent to determine the optimal conditions. There are predictors for the linear DNA migration under Contour Clamped Homogeneous Electric Fields (CHEF) system; however they are not applicable to miniTAFE because the electric field and the reorientation angle formed between the electric field forces lines vary throughout the gel. Therefore, the equation that describes the migration of linear DNA molecules in the gel of CHEF chambers was modified. The values of the electric field at each point of the miniTAFE gel were re-estimated. Also there were considered the variability of the reorientation angle of the TAFE minichamber and the trigonometric relationships between the DNA migrations in each direction of the applied field. The new predictor describes the migration of DNA molecules in the miniTAFE gel. It was adjusted with 254 experimental points and validated by the re-sampling 'leave one out' method. The virtual electrophoretic DNA patterns of known sizes, obtained with the modified equation, reproduced the experimental results with an 86,7 % of efficiency. This equation allows the a priori design of the miniTAFE experimental conditions to separate the DNA molecules with known sequences, facilitating the establishment of working protocols that ensures saving time and chemical reagents. [ABSTRACT FROM AUTHOR]

Se presenta una reseña de un tesis de la autora en el campo de las neurociencias, el cual enfoca en la tipificación molecular de Saccharomyces cerevisiae por un análisis bidimensional y de electroforesis.

Se determinó por DGGE, el efecto de morera (Morus alba vc. cubana) en la población de metanógenos presentes en el líquido ruminal de búfalos de río. Para ello, se realizó una fermentación in vitro y se evaluaron cinco tratamientos que correspondieron con diferentes niveles de inclusión de morera a una dieta base de pasto estrella (PE) (Cynodon nlemfuensis): (1) 100 % de PE (control); (2) PE + 15 % M. alba vc. cubana; (3) PE + 20 % M. alba vc. cubana; (4) PE + 25 % M. alba vc. cubana y (5) PE + 30 % M. alba vc. cubana. Antes del análisis de las muestras, se realizó la estandarización de la DGGE. A las 12 h de fermentación, se tomaron muestras de líquido ruminal, a las que se les extrajo el ADN y se les realizó la DGGE. El gel se escaneó, las imágenes se analizaron mediante el software Bionumerics y se realizaron dendogramas de similitud. Se obtuvo como resultado que los niveles de inclusión de M. alba vc. cubana no produjeron variaciones marcadas en la composición de metanógenos ruminales con relación al tratamiento control, agrupándose todos los tratamientos en un clúster con 81 % de similitud. Se concluye que la inclusión de M. alba vc. cubana no ejerce efecto marcado en la población de metanógenos presentes en líquido ruminal de búfalos de río. [ABSTRACT FROM AUTHOR]

A descriptive study of 129 neonates of both sexes, born in the Neonatology Unit of a Public Health Center from Puerto Cabello city, Venezuelan state of Carabobo, was carried out from January, 2012 to June, 2013, in order to detect early newborns with hemoglobin S. In the series the feminine sex prevailed (51.9%). The electrophoresis technique was used and normal hemoglobin (Hb A) was found in 91.5%, as well as electrophoretic patterns of abnormal hemoglobin compatible with Hb S in 8.5%. Of the 11 positive cases, 90.9% corresponded to Hb SA and 9.1% to Hb SS. The detected hemoglobin SA and SS frequency, even when diagnostic results could not be established, represented a warning to implement the early detection, which conducted to a program of early prevention, and to prophylaxis from the first months of life. [ABSTRACT FROM AUTHOR]

The continued application progress in auto-assembly devices, positioning, sensors, actuators, and controllably allow manipulation of micro and nanostructures. Those have generated wide interest in the development of methodologies to optimize the control manufacturing devices and handling micro and nanoscales. This project explores electrode-manufacturing techniques in order tofind an optimal and reproducible technique. Performances of each technique are compared and cleaning and security protocols are described. Three geometries to mobilize and position micro and nano iron particles in a solution of natural oil were designed and implemented. Finally, electric fields are generated from electrophoresis tofind the curve that describes the particles movement relative to applied potential. These results generate great impact on current manufacturing efforts bottom-up (controlling with golf location) and mobility in electronic devices. Finally, they were made that planar geometry electrodes, it generates the possibility of particles movement are included to integrated circuits manufacturing today. [ABSTRACT FROM AUTHOR]

Cerebral vascular diseases are the third cause of death in Mexico and around the world; including focal cerebral ischemia (FCI) that leads to reduced blood supply in a specific zone of the brain, diminishing the glucose and oxygen. In early FCI stages there are molecular changes not well described in the cells of the affected and surrounding regions; these changes could be detected and used as biomarkers in easily accessed corporal fluids as blood serum (BS), in order to define biomarkers useful for disease advance diagnostic. Objective: analyze protein profile of rat sanguineous serum after 1h of FCI with or without 24h of sanguineous reperfusion (rpf), in order to identify possible early FCI biomarkers. Material and methods: cerebral focal ischemia was experimentally induced in brain rat, BS was obtained and serum albumin and immunoglobulin were depleted. Protein profile of serum samples were obtained by two dimensional gel electrophoresis, the images were analyzed using ImageMaster 2D Platinum software allowing to identify proteins present differentially in FCI. Results: under FCI and rpf, we observed up regulation of five proteins and down regulation of three proteins. To analyze their role as biomarkers in FCI, those proteins will be identified by mass spectrometry. Discussion and conclusion: protein analysis of corporal liquids is useful to find biomarkers to diagnostic and disease control. Proteins changes expression and localization with FCI aggrieving or dismissing injury, and BS could contain diagnostic markers to FCI useful to identify early changes in neurons and glial cells. [ABSTRACT FROM AUTHOR]

We determined the genetic population structure and its implications within the food chain of the copepod Acartia lilljeborgii on the coast of Yucatan, by using isozymes and subsequent determination by electrophoresis. Nine systems were expressed up to 30-loci. Polymorphism and heterozygosity of the populations studied, when compared with other species of copepods were found to be low. The ST2 and PGM1 loci were expressed in all sampling sites. PGM1 showed the highest heterozygosity value (0.4728) and GPI1, with a value of 0.3200 was the lowest value. No system departed from the condition of the Hardy-Weinberg equilibrium. FST values were between values of -0.0798 to 0.5899 and the PGM1 for FBP1. The three populations of A. lilljeborgii analyzed in the coast of Yucatan, had low variability and genetic differentiation among populations, as indicated by the genetic distance values of 0.0585 which was the largest for the populations of Chelem and Rio Lagartos, suggesting that these populations are partially isolated. However, since A. lilljeborgii diapausic or resting eggs, which can be transported in or out of these bodies of water, it is likely that this condition holds allelic and genotypic frequencies similar or very close together. The results showed the existence of gene flow, given through a passive dispersal of diapausic eggs produced by this specie. [ABSTRACT FROM AUTHOR]

Background: Castleman's disease is an atypical lymphoproliferative disorder which may be compatible with paraneoplastic manifestations of POEMS syndrome. Clinical case: a 53 year old man with a history of type 2 diabetes, hypothyroidism and Addison's disease was attended. He started with numbness and weakness in limbs, dyspnea, skin hardening, Raynaud's phenomenon, weight loss and fatigue appeared. Physical exam showed tachypnea, generalized cutaneous hyperpigmentation and skin hardening of extremities, muscle weakness, hypoesthesia and hyporefl exia. Laboratory showed hyperprolactinemia, low testosterone, hypothyroidism and Addison's disease. Electrophoresis of proteins showed polyclonal hypergammaglobulinemia. Somatosensory evoked potentials reported peripheral neuropathy and severe axonal polyneuropathy by electromyography. Chest X-rays showed bilateral reticular infiltrates and mediastinal widening. Echocardiogram displayed moderate pulmonary hypertension. Skin biopsy had no evidence of scleroderma. CT reported axillar, mediastinal and retroperitoneal nodes. The mediastinal lesion biopsy reported hyaline vascular Castleman's disease, multicentric variety. He was treated with rituximab. Conclusions: the case meet criteria for multicentric hyaline vascular Castleman's disease, POEMS variant, treated with rituximab. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The multiple myeloma is a neoplastic disease characterized by the malignant proliferation of plasma cells with frequent infiltration to the bone marrow and other tissues. Cord compression syndrome (defined by thoracic or lumbar pain), changes in strength or sensation, and sphincter disorders are among the early manifestations. It is crucial that clinicians are aware of this possible diagnosis, not only because its omission delays diagnosis, as in the case reported in this paper, but also the correct treatment. Wrong treatments often contribute to increase patient morbidity. When the multiple myeloma presents acute and aggressive behavior it is imperative not to delay the diagnosis. Cord compression syndrome may begin with symmetrical paresthesias of the lower limbs that evolve to paraplegia gradually ascending as infiltration, tumor expansion, and growth of spinal metastases invade the epidural space and the spinal canal. All these clinical manifestations can make the clinician think of a Guillain Barre Syndrome. Misinterpretation of electromyography ends up hindering the proper diagnosis and treatment. In Mexico, intravenous immunoglobulin is used for the treatment of Guillain- Barre syndrome. In a patient with multiple myeloma this would further increase blood viscosity, with subsequent deterioration of renal and cardiac functions. [ABSTRACT FROM AUTHOR]

Objective: To characterize the molecular features of seven extended-spectrum betalactamase-producing (ESBL) strains of Klebsiella pneumoniae in patients with bacteremia during a nosocomial outbreak in a neonatal unit in Lima, Peru. Methods: Genotyping was performed using Eric-PCR, Rep-PCR and pulsed field gel electrophoresis (PFGE) to evaluate clonality. Disk diffusion test showed that all seven strains were ESBL producing strains. Results: The three-genotyping methods showed that five out of seven strains were genetically related to a single clone. Conclusions: The study represents the first attempt to apply genotyping methods to study nosocomial outbreaks in neonatal units in Lima, and reveals clonal transmission of ESBL producing strains of K. pneumoniae. [ABSTRACT FROM AUTHOR]

In this work the hydrolytic activity of the extract from Salpichroa origanifolia ripe fruits on bovine, ovine and caprine caseins was studied. The enzymatic reaction was carried out at pH 6.2 and 37°C of temperature for twenty hours. Peptides obtained from the enzymatic hydrolysis of all caseins were quantified by spectrophotometry and analyzed by tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) and densitometry. Casein hydrolysates were separated by ultrafiltration obtaining higher and lower fractions of 3 kDa molecular mass. Statistical analysis of the caseins hydrolysis was significant for the time of hydrolysis and the type of casein (p<0.05). A higher degree of hydrolysis of caprine casein was observed after 20 hours of incubation. Antimicrobial activity of the hydrolysates was assessed and it was found that less than 3 kDa molecular mass peptides from bovine and ovine caseins showed the highest inhibitory effect on the evaluated microorganisms. [ABSTRACT FROM AUTHOR]

Copyright of Revista Mexicana de Biodiversidad is the property of Universidad Nacional Autonoma de Mexico, Instituto de Biologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

El artículo discurre sobre el aislamiento y la purificación de la toxina de bordetella pertussis y la tosferina. Los autores comentan sobre la producción de las vacunas antipertúsicas, la separación de las proteínas y electroforesis. Otros temas incluyen Western blotting y la detección de los anticuerpos.

Objectives. Determine the genetic diversity of Peruvian isolations of Leptospira spp. through Pulsed Field Gel Electrophoresis (PFGE). Materials and methods. The PFGE methodology proposed by Galloway and Levett (2008) was standardized. A database including the PFGE profiles of 65 reference strains was prepared, and the technique was applied in 111 isolates of Leptospira spp. obtained in Peru between 2002 and 2010. Results. A great generic diversity of serovars of circulating Leptospira spp. was determined in our country. 57 serovars were identified, 47 out of 97 pathogen isolates. Most frequent serovars were Icterohaemorrhagiae/Copenhageni (n=24) and Canicola (n=7). The most frequent species were L. santarosai (49,5%) and L. interrogans (37,1%). The distribution of species, clusters and serovars changed according to the source of isolate, the environmental context and the origin. Conclusions. There is great diversity of circulating serovars in Peru. There are genetic and epidemiological relations among isolates of different sources, and this is related to species, reservoir, environmental context and the origin of the isola [ABSTRACT FROM AUTHOR]

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