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The analysis of blood identification in dry spots is carried out using the Teichmann crystallographic method as a basis to the colorimetric orientation tests in the expert work of the Forensic Biology laboratories of the National Institute of Legal Medicine and Forensic Sciences To determine by Teichmann's crystallographic method its sensitivity, specificity, positive predictive value, negative predictive value, limit of detection, concordance index and Kappa index according to the different conditions of temperature, support and medium in which they are located. 100 samples of dry spots are taken to be analyzed double-blind, in different substrates using the Teichmann confirmatory method for blood identification, following the adequate biosecurity measures by the Forensic Biology Laboratory. The statistical quality values obtained in sensitivity were 96,22%, 100% Specificity, 100% Positive Predictive Value, 96,87% Predictive Negative Value, 1/100 Detection Limit, 100% Concordance Index and a Kappa Index. of 1. Through the data obtained, the effectiveness of Teichmann's crystallographic method as a confirmatory test in support of presumptive methods of orientation in the identification of blood in spots is made manifest. [ABSTRACT FROM AUTHOR]

Leishamnia parasites are the causative agents of the leishmaniasis disease. Twenty species of Leishmania sp has been described as human pathogens and several of them present different clinical manifestations. There is no a protective vaccine for humans. Alternatives to discover new drugs are based on the design of new compounds that bind selected targets. Mainly, the targets are proteins involved in key metabolic or cellular processes of the pathogen, e.g. parasitization of vertebrate host cells. The parasitization of the vertebrate host by Leishmania parasites depends on the expression of different molecules including Lack protein. In this article we highlight the conditions to perform the refold, purification, and crystallizing of the Lack protein of the human pathogen Leishmania (Viannia) panamensis. Moreover, we present structure modelling analysis, the structure of Lack protein was found to be similar to RACK1-ribosome associated protein from Trypanosoma brucei and Saccharomyces cerevisiae and other eukaryotes. The structural characteristics of Lack protein could be used for exploration of new drugs. [ABSTRACT FROM AUTHOR]