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The present research work was carried out with the purpose of characterizing the wild strain of Pleurotus sp. collected in the native community of Korimani, in order to assess its qualities, value and its introduction to the mushroom cultivation biotechnology, using local substrates as a nutritional and productive alternative. The strain was isolated from pseudo-tissue of the fruiting bodies in four solid culture media: Potato Dextrose Agar (PDA), Rice Agar (AA), Potato Carrot Agar (APZ) and Sweet potato Agar (AC). Statistically, the four culture media were optimal for the vegetative propagation of the mycelium at a temperature of 25 °C with a daily growth rate (DGR) of 1.1 cm / day coding as d027-CIPHAM. The basidiomas collected corresponded to the species of Pleurotus djamor (Rumph. ex Fr.) Boedijn. The culture was carried out in local lignocellulosic residues, banana leaves (Musa sp.) and wheat stubble (Triticum aestivum) incubated at 25ºC, obtaining fruit bodies at 7 days after induction under controlled laboratory conditions. According to the physical-chemical analysis carried out, P. djamor presents 19.46% of proteins and 57.31% of carbohydrates, becoming a promising fungal genetic resource for the Cusco region. [ABSTRACT FROM AUTHOR]