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Start Over Descriptor "CEDAR" Topic growth regulators Language spanish
2 results on '"CEDAR"'

1. Efecto de la revigorización en el establecimiento in vitro de ápices y segmentos nodales de Cedrela odorata L.

Author

Jiménez-Terry, Felipe, Barbón, Raúl, Mariana, La O., Pérez, Martha, Collado, Raúl, Acosta-Suárez, Mayra, Alvarado-Capó, Yelenys, and Agramonte, Daniel

Subjects
*CEDAR, *PLANT regulators, *GROWTH regulators, *MICROBIAL contamination, *SODIUM hypochlorite, *DISINFECTION & disinfectants, *BUDDING (Plant propagation)
Abstract

The current research was developed with the objective to decrease the microbial contamination and to increase the survival in vitro of cedar (Cedrela odorata L.). Reinvigoration of mother plants was carried. Sticks of 1-2 cm of diameter and 25 cm of lenth collected in different periods of time were planted in a greenhouse. Growth regulator solutions were applied to evaluate sprouting, and growing of shoots and rootening. New shoots, sprayed with fungicide to eliminate in vitro contamination, were compared with apexes and nodal segments, without fungicide treatment, that were collected directly from field and the green house. Explants were disinfected with sodium hipoclorite (NaClO)to avoid contamination and placed in culture medium with 6-BAP 0.25 mg.l-1 and AIA 0.125 mg.l-1. The use of growth stimulating sustances increased sprouting and growing of axillary buds, and rooting of sticks. A n 84% of survival and 10% of contamination were achieved with disinfection by immersion of explants during 10 minutes in NaClO 1.5%. The necessity of reinvigoration of starting material to achieve in vitro establishment of cedar nodal segments was demonstrated. [ABSTRACT FROM AUTHOR]

Published
2007

2. CRIOCONSERVACIÓN DE ÁPICES Y SEMILLAS DE CEDRO (Cedrela odorata L.) MEDIANTE LAS TÉCNICAS DE VITRIFICACIÓN Y DESHIDRATACIÓN.

Author

García Rojas, Tatiana and Abdelnour Esquivel, Ana

Subjects
*CRYOPRESERVATION of organs, tissues, etc., *SEEDS, *CEDAR, *FOREST products, *PLANT shoots, *LIQUID nitrogen, *GERMINATION, *DEHYDRATION, *PLANTS
Abstract

In order to protect the forest resources of Cedrela odorata L., currently threatened, it was considered necessary to establish a protocol for its cryopreservation. Research was conducted using shoots and seeds. Vitrification techniques were evaluated with shoots, using various solutions and rapid freezing in liquid nitrogen (LN). Initially the best results were obtained using incubation for 5 min in PVS3 solution before freezing, survival being 61% after 3 weeks of culture on Murashige and Skoog (MS) medium with 1.0 mg.l-1 BAP and 0.1 mg.l-1 GA3. Moreover, by incubating the apices in 0.3 M sucrose solution for 5 min prior to freezing, the survival rate reached 86% after 2 weeks of culture on MS medium with 1.0 mg.l-1 BAP, 0.1 mg.l-1 GA3 and 0.01 mg.l-1 IAA. When incubation in the solution of 0.3 M sucrose was used and recovery MS medium with 1 mg.l-1 KIN, 0.5 mg.l-1 GA3 and 100 mg.l-1 activated charcoal, the appearance of the surviving shoots improved significantly. For cryopreservation of cedar seeds, the methodology of dehydration and rapid freezing in liquid nitrogen (LN) was evaluated. Seeds with moisture content between 7.9% and 5.5% were placed in 5 ml polypropylene vials and these were directly immersed in NL. The effect of the storage period in NL (1 h, 24 h, 7 days, 1 month, 3 months and 6 months) on seed germination was evaluated. There were no statistical differences in ability of seeds to germinate after storage during different periods. [ABSTRACT FROM AUTHOR]

Published
2013

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