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Background: the hand hygiene (HH) is the more effective and economical universal is known to prevent transmission of infectious diseases. Objective: understanding the impact of a program of continuous improvement in the process of HH in the health personnel. Methods: shadow, quantitative, prospective and longitudinal study from February 1 to September 30, 2009 at the Regional Hospital "Presidente Juarez" in Oaxaca of ISSSTE, the HH was assessed in 307 health workers, from a database that was evaluated through Rho correlation test of Spearman, with the aid of SPSS 11.5. Results: before training and provision of inputs, health personnel performed hand hygiene procedure in 189 cases (61.5%), while 79.8% did so after training and installation of toilets and hand sanitizer dispensers, liquid soap and paper towels, when an assessment was conduced two months later, 68.4% were conducted HH. At the end of the study were able to observe the program's impact in increasing the percentage of people who applied the HH: in the emergency service increased from 2 to 6%, in the surgery room from 71 to 84% in ICU from 94 to 97%, in pediatric ward from 95 to 99%, in the surgical floor from 4 to 78%, in internal medicine from 44 to 47%, in gynecology from 38 to 49%, in nursery from 89 to 92%, and outpatients from 20 to 29%. The Spearman's Rho test was significant at the three times evaluation. Conclusions: in this study, the pediatric service showed great attachment to comply with the HH, as well as ICU. This reveals that to the extent that there is more material equipment for this purpose, the greater the compliance of the measure which amounted in all services. [ABSTRACT FROM AUTHOR]