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This research study aims to determine the impact of implementing a new bicycle route network proposal on the operability of private vehicles in the city of Manizales (Colombia). Road infrastructure interventions, associated with the implementation of sustainable autonomous transport systems, affect the operability of automotive transport modes because of structural requirements for both safety and operation. The methodology includes applying transport infrastructure supply models as an evaluation basis. This is supported by geographical accessibility, coverage, and geospatial impact analyses. The results show that implementing the proposal generates up to a 3% variation in average travel times for private vehicles. It is concluded that accessibility conditions do no change significantly for private vehicle users. [ABSTRACT FROM AUTHOR]