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Background. Complete blood count (CBC) is a simple and affordable diagnostic tool that is routinely used in therapeutic practice. At the same time, its diagnostic and prognostic potentials in terms of management of acute myocardial infarction (AMI) are currently limited. Based on a number of previous studies, we suggest that the analysis of platelet count and indices among AMI patients may have significant diagnostic and prognostic value, as well as dynamic changes in these parameters during the treatment course.Objectives. To evaluate the value of platelet indices and ratios in terms of their diagnostic and prognostic potential among patients with AMI at the time of hospitalization and on the 7th day of treatment.Materials and methods. The study involved 152 patients diagnosed with AMI (Group 1), 30 patients diagnosed with the chronic coronary syndrome (Group 2) and 24 healthy volunteers (Group 3). CBC was performed for all participants to determine all platelet indices. Also, anamnestic and clinical data were evaluated, as well as results of the coronary angiography. GRACE score was calculated.Results. The highest diagnostic value was shown by the WBC/MPV ratio (AUC 0,798; sensitivity 83,1 %; specificity 65,1 %) and PLT/L ratio (AUC 0,644; sensitivity 62,7 %; specificity 53,5 %), as opposed to traditionally used MPV (AUC 0,426; sensitivity 47,5 %; specificity 48,8 %) and PLT (AUC 0,484; sensitivity 60,8 %; specificity 58,1 %). We were unable to establish a relationship between CBC platelet counts at the time of hospitalization and the risk of complications according to the GRACE score. However, those changes according to CBC were obtained on the 7th day of treatment. In particular, the number of platelets was associated with the risk of hospital (r = 0,287; p = 0,016) and 6-month mortality (r = 0,260; p = 0,30), the duration of ischemia (r = 0,238; p = 0,016) and the number of affected vessels according to the results of angiography (r = 0,395; p = 0,002). Similarly, P-LCC correlated with the risk of hospital (r = 0,483; p = Conclusion. We found that combined ratios such as WBC/MPV and PLT/L had a higher diagnostic value compared to MPV, PLT and P-LCR. The prognostic potential of platelet indices was significantly higher on the 7th day of hospital stay. In particular, elevated levels of platelets, platelets, and P-LCR were correlated with a higher risk of hospital and 6-month mortality.

Samoilova Svitlana O., Plenova Olga M. Peculiarities of platelet hemostasis changes in COPD - hypertensive disease mixed patients. Journal of Education, Health and Sport. 2016;6(4):39-44. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.49394 http://ojs.ukw.edu.pl/index.php/johs/article/view/3445 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.03.2016. Revised 20.03.2016. Accepted: 05.04.2016. Peculiarities of platelet hemostasis changes in COPD - hypertensive disease mixed patients Svitlana O. Samoilova, Olga M. Plenova O.O.Bohomolets National Medical University Abstract Disorders in the hemostasis system have a major impact on the disease course and complication in the hypertensive disease and chronic obstructive pulmonary disease patients. To assess the platelet hemostasis we examined 62 persons: 15 apparently healthy (Control Group), 15 HD patients without COPD, 17 HD patients with COPD associated, and 15 COPD patients of Degree II and III. We have found out that HD, COPD and the mix patients demonstrate an increased thrombocyte functional activity expressed in a higher degree of spontaneous aggregation, and significant activation of arachidonic acid induced thrombocyte aggregation. The HD patients feature a specific increase of adrenalin induced aggregation, contrary to other examined groups, that may be explained by a role of sympathetic nervous system in the HD pathogenesis. Chronic inflammation in the COPD patients is accompanied with a significant increase of arachidonic acid thrombocyte reaction (the highest among all examined groups) that should be taken into account when establishing a treatment policy with anti-inflammatory agents included. Key words: hypertensive disease, chronic obstructive pulmonary diseases, hemostasis, thrombocytes, aggregation.

The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood smears were most acceptable because they allow a differentiation between leukocytes and thrombocytes and also had a smaller variation. The methods investigated here for the Amazon turtle, which have been widely used in other reptile species, provided evidence that the most acceptable method is not that of using diluted stains and a hemocytometer.O objetivo deste estudo foi comparar três diferentes métodos para contar leucócitos totais [LT] (método de Natt & Herrick, de estimação em 1000 e 2000 eritrócitos) e três métodos para contar trombócitos totais [TT] ( método de Wojtaszek, de estimação em 1000 e 2000 eritrócitos) em uma espécie de tartaruga de água doce da América do Sul, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). As contagens diretas de LT usando o método de Natt & Herrick mostraram limitações que são aqui discutidas. As contagens de LT e TT usando estimativa em 1000 eritrócitos na extensão sanguínea não são recomendadas para tartaruga-da-Amazônia nem para outras espécies de répteis, pois houve ampla variação nestas contagens. Os métodos para determinar LT e TT baseados em 2000 eritrócitos nas extensões sanguíneas foram mais aceitáveis porque eles permitem uma diferenciação entre leucócitos e trombócitos, além disso, teve uma variação menor. Os métodos aqui investigados para tartaruga-da-Amazônia, os quais são amplamente usados em outras espécies de répteis, proveram evidências de que o método mais aceitável não é o que usa corantes diluentes e um hemocitômetro.