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Background: NAT genes are considered candidate genes for the genetic predisposition to non-syndromic Cleft lip with or without cleft palate (NSCLP), since they codify for N-acetyltransferases, enzymes responsible for the biotransformation of arylamines, hydrazine drugs, and a great number of toxins and carcinogens present in diet, cigarette smoke, and environment. Aim: To determine the association between alleles determining slow acetylator phenotype and the risk of NSCLP. Material and Methods: We analyzed *5 (481C>T), *6 (590G>A) and *7 (857G>A) alleles which determine the slow acetylator phenotype and *4 (wild type) allele by polymerase chain reaction/restriction fragment length polymorphism in 97 progenitor-case trios of NSCLP in Argentinian Obstetric Wards. We evaluated the transmission disequilibrium (TDT). Results: TDT showed a positive association between allele *5 and NSCLP (odds ratio = 1,6; p = 0,03). Conclusions: The presence of *5 allele is significantly higher in cases with congenital NSCLP., Facultad de Ciencias Naturales y Museo, Instituto Multidisciplinario de Biología Celular

Esta investigación se realizó con el propósito de evaluar el efecto de extractos acuosos de ecotipos de trigo (Triticum aestivum L.) sobre el crecimiento micelial in vitro de Gaeumannomyces graminis var. tritici. Se utilizaron 17 extractos obtenidos de raíces y hojas de los ecotipos de trigo Castaño, Cebada, Diente de perro, G8, Grano de oro, Caín, Linaza I, Linaza II, NN1, NN17, NN24, NN31, NN35, Pulil, R3, Rachel y Varón, y cultivares comerciales de avena cv. Urano, trigo cv. Kumpa y Otto a las concentraciones 1 y 5% p/v. Se evaluó el radio promedio del crecimiento micelial ( mm) desde el centro de la placa petri contenida con APD y el extracto respectivo, siempre en el mismo sentido y lugar, a partir de las 24 horas postsiembra y cada 24 horas hasta completar la placa control. Se utilizó un diseño experimental completamente al azar. Los datos fueron analizados mediante ANDEVA y comparación de medias por las pruebas LSD y Tukey (p < 0,05). Todos los extractos acuosos de ecotipos de trigo ejercieron efecto inhibitorio del crecimiento micelial de G. graminis var. tritici. Este efecto fue superior al de cultivares comerciales de avena cv. Urano y trigo cvs. Kumpa y Otto. La secuencia de mayor a menor efecto inhibitorio del crecimiento micelial fue la siguiente: extracto radical al 1% (ecotipos Varón, NN1 y Pulil); al 5% (ecotipos Linaza I, Caín, Varón, Grano de oro y NN1). Extracto foliar al 1% (ecotipos Castaño y Grano de oro); al 5% (ecotipos NN17, NN24, NN31, Caín y Varón). This research was made with the purpose of evaluating the effect of aqueous extracts of wild type wheat (Triticum aestivum L.) over the mycelial growth in vitro of Gaeumannomyces graminis var. tritici. There were used 17 extracts obtained of root and leaf of wild type wheat: Castaño, Cebada, Diente de perro, G8, Grano de oro, Cain, Linaza I, Linaza II, NN1, NN17, NN24, NN31, NN35, Pulil, R3, Rachel and Varón, also varieties of Kumpa and Otto wheat and (Urano) oats, to concentrations 1 y 5% w/v. There was an evaluation of the radio average of the mycelial growth (mm) from the center of the plate contained with PDA and their respective extract, always in the same sense and place, as of the 24 hours after inoculation and every 24 hours till the plate control was completed. A full-random design was used. The data were analyzed by ANOVA and the average was compared by test of LSD and Tukey (P ≤= 0,05). All the aqueous extracts of roots and leaves of wild type of wheat had an inhibiting effect over the micelial growth of G. graminis var. tritici. The allelopathyc effect over this fungus was superior to the Kumpa and Otto wheat (Urano) oats and (nn) barley. The sequence from greater to smaller inhibiting effect of the micelial growth was the following one; radical extract to 1% wild type of wheat (Varón, NN 1 and Pulil); to 5% (Linaza I, grano de oro, Varón, and NN 1). Extract to leaf to 1% (Grano de oro and castaño); to 5% (NN17, NN 24, NN 31, Cain and Varón).