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El final de la serie Gomorra, brutal estampa de la mafia napolitana basada en la novela de Roberto Saviano, nos invita a reflexionar sobre algunos aspectos urbanísticos y arquitectónicos de la ciudad de Nápoles, verdadera protagonista del relato. A partir de la ficción, nos remontamos a los orígenes urbanísticos de la periferia de Nápoles y cómo la mafia impuso su control socio-económico. Gomorra establece una relación dual de documentación y dramatización del patrimonio arquitectónico de la ciudad. En la serie vemos reflejada la importancia de la relación entre los espacios domésticos y urbanos y la constante disputa entre pecado y redención que rige el destino de Nápoles.

Objective: To analyse sportsmanlike and unsportsmanlike conduct of football players in juvenile category (aged from 10-12), as well as sanctioning of players and their trainers. Methodology: Observation of thirty teams during fifteen football games (1050 minutes) has been conducted. Tools used in process of research were observation and study of documents. Results: Results reveal some interesting considerations to have in mind in Grassroots Football: (1) the trainers are the group that is sanctioned most frequently, (2) majority of sportsmanlike behaviour, such as initial greeting occurs before starting the game, while during the game it is not as recurrent, (3) only half of the teams says goodbye to their opponents after the match, (4) players usually do not react sportsmanlike when they commit a foul and (5) major unsportsmanlike conduct is linked to the referees, either due to protesting to their ruling or by trying to deceive them. Implications: Sports at young age should be educational, trainers should be good role models to their players and football player should exhibit sportsmanship. Mentioned can be displayed by raising their hand to acknowledge their foul or offering their hand in order to apologize, help the injured player, accept victory praise or be courteous to the defeated player.

El objetivo de este trabajo es validar el cuestionario creado para analizar la ocupación del tiempo libre de jóvenes y adolescentes, a través de la participación o no en actividades extraescolares una vez finalizada la jornada lectiva. En Extremadura, existe la posibilidad de elegir la modalidad de jornada escolar con actividades formativas complementarias (AFC) que comprende actividades lectivas en horario de mañana y actividades formativas complementarias en horario de tarde. En consecuencia, se podría afirmar que los escolares han visto incrementado su tiempo libre. En el estudio han participado escolares (n= 773) del tercer ciclo de educación primaria y de educación secundaria de diferentes centros públicos y concertados de Badajoz y Poblados. Se llevó a cabo un proceso de construcción, pilotaje y validación del cuestionario a través del análisis estadístico de los ítems, sometido a valoración por un grupo de expertos y prueba por el grupo experimental, cumpliéndose los requisitos de validez externa, expresados en la representatividad de la muestra de la población objeto de estudio, validez interna equivalente al concepto de calidad de encuesta y validez de contenido expresado en el correcto diseño y elaboración del cuestionario definitivo.

El objetivo principal de este estudio ha sido analizar el grado de influencia de la trayectoria académica y de algunos hábitos relacionados con el entorno escolar sobre el rendimiento académico en educación física (E.F.) en estudiantes de educación secundaria obligatoria (ESO) de la ciudad de Ba­dajoz. Una muestra total de 1197 alumnos de enseñanza secundaria obligatoria con un 49.9% hombres y un 50.1% mujeres, ha participado en el estudio. Se les pasó un cuestiona­rio autoadministrado con preguntas sobre sus principales hábitos escolares, de los cuales han sido analizadas ocho variables, además se incluyó la nota final de curso de la asignatura de E.F como variable de análisis del rendimiento académico. A través del análisis estadístico con ANOVA, U de Mann-Whitney y H de Kruskal Wallis, se observan diferencias estadísticamente significativas (pd».001) en las calificaciones en E.F. tanto en las variables que miden la trayectoria académica, como en los hábitos relacionados con el entorno escolar, entre las que destacamos: ser repetidor, ser absentista, el tiempo de lectura y el de estudio diario. Los alumnos que eran repetidores, faltaban más a clase, o se retrasaban un mayor número de veces, que leían y estudiaban menos y percibían un peor ambiente en sus clases, son los que obtenían un peor rendimiento en E.F.. Palabra clave: adolescentes, rendimiento académico, educación física, enseñanza secundaria obligatoria, Badajoz, estudio, lectura. Abstract: The main objective of this study was to analyze the degree of influence of some school habits and scholar trayectory on academic achievement in physical education (PE) students in secondary education (ESO) in the city of Badajoz. A total sample of 1197 students in compulsory secondary education 49.9% men, and 50.1% women, participated in the study. They spent a questionnaire filled out by the river questions about major school habits, of which eight variables were analyzed also included the final course in the subject of EF as a variable for analysis of academic performance. Through statistical analysis with ANOVA, Mann-Whitney U, and Kruskal Wallis H, there are significant differences in PE scores in all variables analyzed (p d».001), among which being repetitive, being truant, the time to read and study daily. In the variable environment perceived in class, there is a degree of significance (p d».05). Pupils who were repeaters, missing more classes or were delayed more times than read and studied less and earned a worse environment in their classes, are those who obtained poorer performance on EF. Key words: teenagers, academic achievement, physical education, compulsory secondary education, Badajoz, study, reading.

El objetivo de este trabajo es validar el cuestionario creado para analizar la ocupación del tiempo libre de jóvenes y adolescentes, a través de la participación o no en actividades extraescolares una vez finalizada la jornada lectiva. En Extremadura, existe la posibilidad de elegir la modalidad de jornada escolar con actividades formativas complementarias (AFC) que comprende actividades lectivas en horario de mañana y actividades formativas complementarias en horario de tarde. En consecuencia, se podría afirmar que los escolares han visto incrementado su tiempo libre. En el estudio han participado escolares (n= 773) del tercer ciclo de educación primaria y de educación secundaria de diferentes centros públicos y concertados de Badajoz y Poblados. Se llevó a cabo un proceso de construcción, pilotaje y validación del cuestionario a través del análisis estadístico de los ítems, sometido a valoración por un grupo de expertos y prueba por el grupo experimental, cumpliéndose los requisitos de validez externa, expresados en la representatividad de la muestra de la población objeto de estudio, validez interna equivalente al concepto de calidad de encuesta y validez de contenido expresado en el correcto diseño y elaboración del cuestionario definitivo. Palabra clave: Tiempo libre, ocio, actividades extraescolares. Abstract: The objective of this work is to validate the questionnaire that was produced to analyze the use of leisure time by youths and adolescents, by participating or not, in extra-curricular activities at the end of the school day. In Extremadura, it is possible to elect the shape of the school day with formative complimentary activities (DOE, 2002) which include school activities during the morning timetable and formative complimentary activities during the afternoon timetable. And, as a consequence it has been proved that school children have seen their leisure time increase. In this study, 773 school children have taken part, both from the third year of primary school and the compulsory secondary school from different state schools in Badajoz and the provinces. The construction, piloting and validation of the questionnaire was carried out by means of the statistical analysis of the items, which was assessed by a group of experts and tested by the experimental group. The necessary requisites were fulfilled as follows; external validation expressed in the representative sample of the population which were the object of this study, internal validation equivalent to the concept of quality of the questionnaire and content validation manifest in the correct design and elaboration of the definitive questionnaire. Key words: Leisure time, leisure, out of school activities.

Objective.: To evaluate in silico and at the serological level the antigenic potential of the recombinant extracellular domain of the lipopolysaccharide assembly protein - D (LptD) of Bartonella bacilliformis (dexr_LptD)., Materials and Methods.: Through in silico analysis, we selected a B. bacilliformis protein with antigenic and immunogenic potential. The selected protein gene was cloned into Escherichia coli TOP10 and expressed in Escherichia coli BL21 (DE3) pLysS. Recombinant protein was expressed using isopropyl-β-D-1-thiogalactopyranoside (IPTG) and induction conditions were optimized. Finally, it was purified with Ni-IDA resin (His60 Ni Superflow) and a Western Blot assay was conducted., Results.: In silico, the selected protein was LptD because it is located in the outer membrane and is antigenic and immunogenic. Optimized conditions for dexr_LptD induction were 0.5 mM IPTG, 16 hours, TB (Terrific Broth) medium, 3% (v/v) ethanol, 28 ºC, OD600: 1-1.5 and 200 rpm. Purification was carried out under denaturating conditions on a small scale and we obtained 2.6 μg/mL of partially purified dexr_LptD. The Western Blot assay showed a positive reaction between the sera from patients with Carrión's Disease and dexr_LptD, which shows the antigenicity of dexr_LptD., Conclusions.: The dexr_LptD shows antigenicity both in silico and at the serological level, these results are the basis for further studies on vaccine candidates against Carrion's Disease.