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This study is focused on morphology and distribution of species of the genera Berkeleya, Amicula, Cocconeiopsis, Haslea and Fallacia from the temperate marine coastal waters of the Gulf of San Matías (Prov. of Rio Negro, Argentina). Material was collected with 30 μm net hauls and analyzed in light and scanning electron microscopy. The genus Amicula and two species A. specululum and Fallacia margino-punctata are reported for the first time from South American coastal waters. The genus Cocconeiopsis and C. pullus are reported for first time for Argentina and Haslea quarnerensoides is reported for the first time from South America.