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OBJETIVO: Descrever a atividade de hematofagia de Aedes aegypti e Aedes albopictus em área submetida ao controle e à vigilância entomológica. MÉTODOS: O estudo foi realizado de abril de 1993 a setembro de 1994, na cidade de Cosmópolis, Estado de São Paulo. Utilizou-se isca humana para coleta dos exemplares. Calculou-se o número médio mensal de fêmeas capturadas por domicílio segundo as áreas central e periférica do município e os locais intra e peridomiciliar. Apresenta-se o índice pluviométrico mensal sem ajuste de modelo. RESULTADOS: Observou-se a presença de fêmeas das espécies em 83% (Ae. albopictus) e 61% (Ae. aegypti) do período estudado. Os meses de janeiro, fevereiro e março apresentaram maior atividade de fêmeas para as duas espécies, com maior presença de Ae. albopictus do que de Ae. aegypti. Os exemplares foram capturados na área central e periférica da cidade, nos períodos matutino, das 9:00 às 12:00 e crepuscular vespertino, das 16:00 às 19:00. Com utilização de modelo de regressão de Poisson, observou-se que fêmeas de Ae. albopictus foram mais capturadas no peridomicílio, independente se área central ou periférica. CONCLUSÕES: Foi detectada diferença no perfil de atividade da hematofagia somente para o Ae. albopictus, sendo de relevância o peridomicílio.OBJECTIVE: To describe the hematophagous activity of Ae. aegypti and Ae. albopictus in an area under control and surveillance. METHODS: The study was conducted during 18 months, from April, 1993 to October, 1994, in Cosmópolis, São Paulo state, Brazil. Human baits were used to collect mosquitoes. The number of females captured is presented monthly by area of the city and local in the household. The rainfall was measured and indices are presented without model adjustment. RESULTS: The presence of females of both species was observed in 83% (Ae albopictus) and 61% (Ae. aegypti) of the period studied. The months of January, February and March presented the highest rates of activity for females of both species, with Ae. albopictus being more frequently captured than Ae. aegypti. Both species were captured in central and peripheral areas of the city, during the day from 9 to 12 am and from 4 to 7 pm. By a Poisson regression, it was observed that Ae. albopictus females were more frequently captured in the peri-housed area, in an independent way considering the area of the city. CONCLUSIONS: It has been identified difference on the hematophagous activity only for Ae. albopictus, being of importance the outside area of the house.

This study describes the isolation and structural determination of two amides, isolated for the first time: N,4-dihydroxy-N-(2'-hydroxyethyl)-benzamide (0.019%) and N,4-dihydroxy-N-(2'-hydroxyethyl)-benzeneacetamide (0.023%). These amides, produced by the red macroalgae Bostrychia radicans, had their structures assigned by NMR spectral data and MS analyses. In addition, this chemical study led to the isolation of cholesterol, heptadecane, squalene, trans-phytol, neophytadiene, tetradecanoic and hexadecanoic acids, methyl hexadecanoate and methyl 9-octadecenoate, 4-(methoxymethyl)-phenol, 4-hydroxybenzaldehyde, methyl 4-hydroxybenzeneacetate, methyl 2-hydroxy-3-(4-hydroxyphenyl)-propanoate, hydroquinone, methyl 4-hydroxymandelate, methyl 4-hydroxybenzoate, 4-hydroxybenzeneacetic acid and (4-hydroxyphenyl)-oxo-acetaldehyde. This is the first report concerning these compounds in B. radicans, contributing by illustrating the chemical diversity within the Rhodomelaceae family.

Effects of nitrate availability in two culture media (von Stosch (VSES), and artificial ASP 12-NTA), and nitrogen sources (seawater enriched with nitrate, ammonium or urea in concentrations ranging from zero to 30 µM) were evaluated in two brown morphs (BR-1, BR-2), one light-green morph (LG) and one dark-green morph (DG) of Hypnea musciformis (Wulfen) J.V. Lamour. Higher growth rates of the four morphs were observed in VSES medium. However, artificial ASP12-NTA medium induced tetrasporangium development in DG morph. Growth rates of the four colour morphs followed kinetic of saturation-type nutrient uptake in treatments with urea. In contrast, growth rates of BR-1, BR-2 and LG morphs were inversely proportional to ammonium concentrations, and those higher than 15 µM were lethal. Growth responses of colour morphs of H. musciformis showed intraspecific variations, and they could be used as bioindicators of nitrogen pollution in marine environment by their low tolerance to ammonium.

The aim of this study was to detect Brucella spp. in artisanal cheese commercialized in Parnaíba city, Piauí state, Brazil. For this study, 30 samples of curd cheese (500g) were randomly collected from different points in the commercialization process. In the laboratory, 25g aliquots of the samples were suspended in Brucella broth; after this procedure, 10µL aliquots of this suspension were sown in Thayer Martin agar plates that were supplemented with 10% defibrinated sheep blood and VCNT antimicrobials. After inoculation, the samples were incubated at 37°C in microaerophilic conditions for 14 days. The suspected morphological colonies were identified and confirmed by polymerase chain reaction (PCR). From thirty samples of microbiologically analyzed cheeses, six samples (20%) were confirmed by PCR as bacteria from the Brucella genus. The detection of Brucella spp. was confirmed in cheese commercialized in markets or a public square (3.33%), bakery (3.33%) and small market (13.33%). Brucella spp. was detected in artisanal cheese commercialized in different points from Parnaíba city. Guidelines for the establishment of good practices for the production of artisanal cheese should be determined by the competent authorities. However, for the better control of human brucellosis it is necessary to control or eradicate bovine brucellosis in the herds.

Abstract This study focused on the detection of anti-Neospora caninum IgG antibodies in cows in the dairy farming region of the state of Piauí, Brazil. To this end, serum samples were collected from 255 dairy cows on 17 farms located in the dairy farming region of the municipality of Parnaíba, Piauí, Brazil. The indirect fluorescence antibody test (IFAT) was employed to detect anti-N. caninum IgG antibodies, using anti-bovine IgG (Sigma®) conjugated to fluorescein isothiocyanate (FITC) and a cutoff point of 1:200. Of the 255 samples analyzed, 69 (27.06%) were positive for anti- N. caninum IgG antibodies, the relative frequency found by property was: 1 (20.00%), 2 (53.33%), 3 (46.66%), 4 (53.33%), 5 (26.66%), 6 (6.66%), 7 (6.66%), 8 (20.00%), 9 (26.66%), 10 (26.66%), 11 (20.00%), 12 (20.00%), 13 (46.66%), 14 (26.66%), 15 (26.66%), 16 (20.00%) and 17 (13.33%). with titers of 200 (15.94%), 400 (20.30%), 800 (24.63%), 1600 (23.18%) and 3200 (15.94%), being the highest frequency for the titer of 800. This study demonstrates for the first time that cows from dairy herds of Parnaíba municipality, state of Piauí, are exposed to N. caninum.

Tendo como foco discutir concepções de juventudes que se expressam em políticas públicas para jovens no Brasil, concentramos nossa pesquisa no Consórcio Social da Juventude. A partir da perspectiva da Psicologia Social, usamos como estratégia metodológica a problematização e como ferramenta teórica conceitos de Michel Foucault. Buscamos compreender como as práticas discursivas instituídas em políticas públicas definem e denominam parcelas da juventude. Ao problematizarmos as tensões elencadas do material analisado, discutimos quatro concepções de juventude: voluntária, digitalizada, trabalhadora e vulnerabilizada. Estas se constituíram em profícuos indicadores de análise para a problematização dos enunciados dos documentos e entrevistas. A análise sinalizou o quanto as políticas públicas de juventude estão inseridas em uma rede discursiva que desenha, forma e institui modos de viver de jovens - de baixa renda - na contemporaneidade. Observamos que, de forma generalizada, discursos de educação, cidadania, inclusão, entre outros, operam, principalmente, para legitimar programas de políticas públicas.Focusing on concepts of youth expressed in government policies for young people in Brazil, we concentrated our research in the Consórcio Social da Juventude - a national project developed by the Brazilian Government. From the Social Psychology perspective, we used the problematization as a methodological strategy and concepts of Michel Foucault as theoretical tools. We sought to comprehend how the discoursive practices installed in government policies define and establish portions of young people. Problematizing the tensions verified in the material analysed, we discussed four concepts of young people: volunteer, digitalized, worker and vulnerabilized. They turned into proficuous indicators of analysis for the proposed problematization. The analysis suggests how government policies are inserted in a discoursive net that designs and constitutes ways of living of young people - especially those of low income. We observed that discourses of education, citizenship, inclusion, among others, operate to offer credibility to programmes of government policies for young people.

Chemical investigation of the dichloromethane/methanol extract of the marine alga Bostrychia tenella has led to the isolation of two aromatic compounds, the new sulfate metabolite potassium 4-(hydroxymethyl)-benzenosulfonate (1) and the compound 1-methoxyphenethyl alcohol (2), described previously as a synthetic product. Their structures were determined by spectroscopic methods including NMR, MS, IR and by comparison with literature data.