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ABSTRACTThis study aimed to investigate the impact of microwave drying (MD), microwave vacuum drying (MVD), freeze drying (FD), vacuum drying (VD), hot air drying (HAD), and sun drying (SD) on the quality attributes of freeze-thaw pretreated beetroots. The quality characteristics including physical properties, bioactive compounds, and antioxidant activities of dried beetroots were evaluated. The results showed that the freeze-thaw pretreated beetroots obtained using FD displayed the lowest shrinkage rate, hardness, and total color difference (ΔE), and the highest rehydration ratio of 6.49. FD, MD, and MVD resulted in porous structures of freeze-thaw pretreated beetroots. The highest betalains, ascorbic acid, total phenolic and total flavonoids contents were found in freeze-thaw pretreated beetroots prepared by MVD. Meanwhile, the largest FRAP value and ABTS free radical scavenging ability of freeze-thaw pretreated beetroots were obtained by MVD. These findings suggested that MVD is a more favorable method for drying freeze-thaw pretreated beetroots.

Lactic Acid bacteria (LAB) are the main producers of bioactive compounds (peptides, exopolysaccharides, antioxidant compounds, gamma-aminobutyric acid-GABA, etc.). Different strains produce at least one metabolite in high quantities as part of their metabolism, however, there are few reports of LAB with the capacity to produce two or more metabolites simultaneously. Due, the ability of 35 LAB strains previously isolated from dairy products, fermented beverages, and honey to produce gamma-aminobutyric acid (GABA) and the antioxidant capacity was determined. Based on analysis of variance and subsequent comparison of means, the Lactiplantibacillus pentosus strain was selected, and subsequently, using a Draper-Lin small composite design, the conditions of the culture medium (MRS broth) were optimized to achieve the highest production of GABA and increase the antioxidant capacity evaluated by the DPPH assay. The optimal conditions for obtaining 7.99 mg/mL of GABA were 100 mM glutamic acid, a pH of 6, a temperature of 40 °C, and 72 h of fermentation. To obtain the highest antioxidant capacity (6.87 µmol TE/mL), 200 mM glutamic acid and a pH of 4.0 were used. We reported an isolated strain of nondairy product (L. pentosus TEJ4) with the capacity to produce both bioactive, GABA, and antioxidants in a single fermentation process in a non-dairy defined medium, which is a feasible alternative to the normal process of producing bioactive compounds.

Context: SARS-CoV-2 spike (S) protein, governed by its receptor binding domain (RBD), is a key player in mediating viral attachment and fusion into host cells, which leads to infection and transmission of COVID-19. Searching for effective inhibitors against S RBD protein is essential to stop the virus infection. Aims: To evaluate Kaempferia parviflora’s bioactive compounds as inhibitors against SARS-CoV-2 S RBD protein and its complex with human angiotensin-converting enzyme 2 (ACE2) receptor through in silico, and to determine the ability of K. parviflora’s extract to inhibit the binding of S RBD and ACE2. Methods: Molecular docking was performed to evaluate the inhibition potentials of K. parviflora’s bioactive compounds against S RBD protein and its complex with ACE2. The inhibitory activity of K. parviflora’s extract against the binding of S RBD and ACE2 was determined using an in vitro inhibition assay. Results: K. parviflora’s compounds had inhibition potentials against S RBD protein in both closed and open states. In the open RBD, these compounds were bound to the key amino acids that were involved in the binding of RBD with ACE2, suggesting their possible roles in preventing the RBD-ACE2 association. K. parviflora’s compounds also had strong affinities towards the S RBD-ACE2 complex by interacting with the paired RBD-ACE2 amino acids, which were crucial for the S RBD-ACE2 complex’s stability. In addition, K. parviflora extract also demonstrated inhibitory activity against the binding of S RBD and ACE2. Conclusions: This study proposes K. parviflora as a promising inhibitor against SARS-CoV-2 S protein for the treatment of COVID-19.

This review article explores various aspects of sweetpotatoes and their leaves, including their production, nutritional composition, health benefits, culinary uses, and future potential as a superfood. Often hailed as a nutrient powerhouse, sweetpotatoes have recently gained popularity due to their exceptional dietary profile and culinary versatility. As a starchy root vegetable from the Convolvulaceae family, sweetpotatoes are consumed worldwide and are integral to many cultures and cuisines. Their vibrant colors, ranging from orange to purple, enhance visual appeal and signal their rich nutritional value. Sweetpotatoes contribute significantly to human health, solidifying their status as a superfood. With endless culinary possibilities, they are an essential addition to a well-balanced diet. Looking ahead, research and innovation aim to enhance sweetpotatoes’ nutritional value, develop climate-resilient genotypes, and promote sustainable production, ultimately contributing to food security, economic growth, and healthier diets globally. The expanding market underscores the urgency of prioritizing these research areas.

Fruits of Schinus terebinthifolia Raddi were investigated. A fast and efficient method for the simultaneous determination of 11 polyphenolic substances by high performance liquid chromatography-diode array detector (HPLC-DAD) was applied. Limits of quantification ranged between 0.78 and 3.14 mg L-1. High concentrations of kaempferol were found. An exploratory evaluation was performed using principal component analysis (PCA) for fruit origin differentiation. Total phenolic content (TPC) ranged from 274 to 517 mg GAE 100 g-1; total flavonoid content (TFC) ranged from 101 to 283 mg QE 100 g-1. In vitro analyses showed that antioxidant capacity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) ranged from 1.7 to 7 µM DPPH 100 g-1. The bacteriostatic and bactericidal effect were also evaluated against: Bacillus subtilis, B. cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Candida albicans and C. glabrata, using broth microdilution assay. It can be inferred that S. terebinthifolia Raddi fruit has the potential to act as an ally in the search for new alternatives in the action against pathogens, and it can be used as a potential natural antioxidant and antimicrobial agent. Metals (Cu, Fe, Mg, Mn and Zn) were also determined by flame atomic absorption spectrometry (FAAS), with emphasis on high concentrations of Mg, Zn and Fe.

We aimed to study the obtention of valuable phenolic compounds from tissue by-products of agro-industrial processing of apple (GS) and strawberry (RF) using green solvents and Soxhlet extraction methodology. The effects of solvent type [water (W); 80% ethanol, (EtOH)] and extraction ratio (1:10, 1:20, 1:30, and 1:40 p/v) were determined on total phenolic content (TPC), antioxidant capacity (DPPH), and the profile of phenolic compounds of the GS and RF extracts. The solvent type and the extraction ratio significantly affected TPC and DPPH of GS and RF extracts. Extraction with EtOH and 1:40 ratio produced the highest yields, obtaining an RF extract with 15.8 g GAE/Kg (TPC) and 19 mmol TE/Kg (DPPH). The tetra-galloyl glucose isomer and agrimoniin (0.8-0.4 g/Kg) were the main RF phenolic compounds of the eight identified. GS extract, obtained with EtOH and 1:40 ratio, had 11.9 g GAE/Kg (TPC) and 20.5 mmol TE/Kg (DPPH), having quercetin -3-o-glucuronide (0.43 g/Kg) the highest concentration among the eight phenolic compounds identified. The results highlight the potential of green solvents to obtain valuable compounds from low-cost raw materials, like the high-antioxidant capacity phenolic compound extracts obtained herein. Highlights: • Valuable bioactive compounds can be extracted from strawberry and apple agro-industrial waste. • Extraction parameters affected phenolic compound profile and antioxidant capacity. • Apple and strawberry by-products are low-cost sources of hydrolysable tannins and flavonols. • Green solvents used for biowaste valorisation can help in circular economy transformation.

Using crop by-products has become a key line of research in the current circular economy and bioeconomy scenario, specifically for extracting bioactive compounds of interest in designing and developing value-added products for food, cosmetics, health, biofuels, and animal feed industries. In Colombia, harvest and postharvest residues and crop surpluses have been underutilized as a potential source of bioactive compounds. Thus, the objective of this research is to analyze research insights and technologies used to take advantage of by-products from broccoli (Brassica oleracea var. italica) cultivation through a methodological design that combines scientific intelligence, surveillance technology, and scientometric analysis methods and tools. We identified the current lines of research in postharvest and processing and the respective technologies in the topics of senescence, packaging and transport, shelf life, quality, drying process, and by-products, as well as research insights and technologies in health, integrated crop management, and genetics. Furthermore, the scarcity of research results on broccoli by-products and their use is highlighted, showing an opportunity to strengthen research on this topic. Finally, the challenges and future work for research on the exploitation of broccoli by-products are established based on the use of their bioactive compounds in value-added products.

ABSTRACTHeating by microwave irradiation could be an alternative for cooking quinoa and improving its content of phenolic compounds, which have health-promoting properties. The goal of this study was to improve the bioaccessibility of phenolics content and antioxidant activity of quinoa using an optimized method of microwave irradiation. The best microwave irradiation conditions obtained by maximizing the responses were 100% irradiation power and 14 min of irradiation time. The predicted and experimentally validated values for total phenols, flavonoids, and antioxidant activity were as follows: 686.46 vs 749.67 ± 36.62 µg GAE/g; 743.94 vs 803.37 µg QE/g and 4.20 vs 4.61 µM TE/g, respectively. At the gastric level, heating by microwave irradiation promoted the bioaccessibility (>100%) of total phenols, flavonoids, and antioxidant activity, as well as gallic and coumaric acids, quercetin, and kaempferol greater. The heating treatment by microwave in quinoa could be useful to obtain new products with bioactivity potential at gastric and intestinal levels.

ABSTRACTFungi can thrive in strawberries, mainly Botrytis cinerea, which causes relevant fruit losses. Moreover, thermal preservation results in phytonutrient losses. This study evaluated the effects of high hydrostatic pressure (HHP, 0, 400, and 600 MPa/5 min) processing with potassium sorbate (PS) or avocado seed acetogenins (ASA) on the nutritional, microbiological, and physicochemical properties of strawberry puree inoculated with Botrytis cinerea. Sole inoculation reduced L-ascorbic acid (L-AA, 84%) and total ascorbic acid (TAA, 38%). Instrumental color Hue angles were reduced from 35.0 to 29.4°, likewise the contents of pelargonidin−3-glucoside (25%) and ellagic acid (38%). Both HHP-PS treated samples contained no viable conidia and high TAA; the no-preservative and HHP-ASA (661 µg/mL, 400 MPa/5 min) purees contained high TAA but had viable conidia, which can induce further phytonutrient losses. HHP and antifungal additives maintained ellagic acid, increased pelargonidin−3-glucoside contents, and inactivated conidia, strengthening the value of combined non-thermal technologies for the preservation of strawberry puree

ABSTRACTThe potato is a tuber with high global demand and importance in the human diet. It is used by food industries to manufacture processed products (French fries, chips, and purees), and consequently, significant quantities of potato peel are generated as a by-product. Potato peels are rich in compounds like anthocyanins, glycoalkaloids, phenolic compounds, and flavonoids. These bioactive compounds are associated with good health because they protect the body’s cells from oxidative processes and have antioxidant, anti-inflammatory, antitumor, and anticancer properties. Hence, new methods to recover these compounds and their application to food matrices are being explored. This review aims to raise awareness of the value of potato peels as a source of different phenolic compounds and the need to recover them. The review highlights the most recent research on the use of this by-product in different foods to add value to the final composition or improve technological and nutritional properties.

ABSTRACTKombucha is a fermented tea known for its health-enhancing properties owing to the bioactive compounds generated by acetic acid bacteria (AAB) and lactic acid bacteria (LAB). We compared the distribution of AAB and LAB across nine kombucha products in Singapore (Product A to I) using shotgun metagenomics. High prevalence of Komagataeibacter species including Komagataeibacter saccharivorans (82.93% in B), Komagataeibacter xylinus (93.38% in D), and Komagataeibacter rhaeticus (92.20% and 30.62% in G and I) was detected in AAB-dominant kombucha. LAB-dominant kombucha was represented mainly by Bacillus coagulans (~99% in E and F) and Lactobacillus nagelii (~60% in H). Despite differences in bacterial composition, all kombucha harbour pathways involved in the biosynthesis of short-chain fatty acids (SCFAs), amino acids and vitamin B12. Interestingly, “fatty acid and beta-oxidation II (peroxisome)” and “fatty acid and beta-oxidation I” were only present in LAB-dominant kombucha. Further study is required to elucidate the significance of the discrepancies.

ABSTRACTThe extraction of bioactive compounds from natural food sources through non-conventional novel methods is gaining popularity due to their numerous advantages over conventional methods. In this review article, various extraction methods such as conventional and non-conventional novel methods were discussed with emphasis on green extraction methods. The use of hybrid extraction techniques has an effective and novel method of extraction. The bioactive compounds extracted by such novel techniques have great potential in functional food market, as they are considered as safer. Additionally, with an increasing interest of consumers toward food product, these novel greener technologies would be safer since it has less chemical interference. The outcome of this review will give an idea for appropriate extraction process with better efficiency and eco-friendly, which could be beneficial for extraction industry. Further research is required to validate these green extraction techniques in terms of their safety, consumer acceptability, challenges, and legal feasibility.

ABSTRACT Du e to the concern with the contamination of food by pesticides, especially coffee, the aim of this study was to analyze the chemical composition and glyphosate residue in Conilon coffee beans with and without defects and harvested at different times after applying the herbicide. The experiment was in a split-plot design, with the presence or absence of defects in the beans comprising the subplots, and the periods of 15, 30, 45 and 60 days after applying the herbicide corresponding to the split plots. We analyzed the volatile compounds, bioactive compounds, and glyphosate residue in the beans. Regardless of the presence or absence of defects, there was an increase in the concentrations of bioactive compounds in the raw Conilon coffee beans as the interval between applying the herbicide and harvesting the beans was increased. The most abundant volatile compounds per percentage area belonged to the pyrazines, furans and phenols. The amount of glyphosate residue found in the beans exceeded the maximum detectable limit by the ELISA method regardless of the time between application and harvest, corresponding to values that are unacceptable to several purchasing countries, and making the samples unsuitable for export and a risk to food safety.

Introduction: Ficus pumila and Phtirusa stelis are emerging as a source of natural products. Objectives: Identify the families of chemical compounds present in the species to be studied. Determine the antibacterial and antifungal activity of the crude extracts of F. pumila and P. stelis. Assess the toxicity of the crude extracts of the species against Artemia sp. Determine effects on Aedes aegypti larvae and. Methods: We evaluated the bioactivity of ethanolic, and isopropyl extracts obtained from the fruits, leaves and stems of both species were evaluated for antibacterial activity, antifungal activity, toxic activity against Artemia sp, A. aegypti larvae and phytochemical study. Results: We identificated flavonoids, tannins, and polyphenols in isopropyl and ethanol extracts of leaves, stems and fruits of F. pumila. We detected flavonoids, tannins, anthraquinones, triterpenes, and polyphenols in isopropyl and ethanol extracts of stems and leaves of P. stelis. We detected flavonoids, tannins, anthraquinones, triterpenes, and polyphenols in isopropyl and ethanol extracts of stems and leaves of P. stelis. F. pumila, the isopropyl stem extract demonstrated the most effective activity, since it exerted antibacterial activity, with inhibition halos being observed on B. subtilis (10 mm) and E. coli (7 mm), while the ethanolic extract of the leaves showed effect on Staphylococcus aureus (11 mm). The ethanolic extract of P. stelis leaves exhibited activity against S. aureus (10 mm), B. subtilis (10 mm) and that of stem extract showed similar results with inhibition zone of halos of 11 and 10 mm, respectively. The isopropyl extract of leaves also displayed activity against E. coli (15 mm). None of the extracts from F. pumila and P. stelis, showed any growth inhibitory effect against the pathogenic fungi. The majority of P. stelis extracts showed positive LC results against Artemia sp., with the ethanolic leaf extract exhibiting high toxicity (0,01 µg/mL). The isopropyl leaf extract also showed high toxicity (0,01 µg/mL), as did the isopropyl stem extract (0,02 µg/mL). The ethanolic and isopropyl extracts of F. pumila and P. stelis did not show toxicity to Aedes aegypti larvae. Conclusion: The plant species F. pumila and P. stelis, can be considered as sources of important and promising compounds for therapeutic research. These plants have shown potential in various studies, and further research could lead to the discovery of new treatments and therapies.

Abstract This study aimed to understand the perception of young adults and middle-aged adults about functional foods and to relate it to socioeconomic, health, and consumption factors. The study participants, both male and female adults (n = 522), completed two questionnaires. The first questionnaire addressed socioeconomic and health aspects, which were designed by the researchers. The second questionnaire focused on attitudes related to the consumption of functional foods, evaluating consumers' perceived needs, benefits, confidence, and safety. The results demonstrated that adults hold a positive perception of functional foods, acknowledging their benefits and the need for consumption. However, there is some skepticism regarding the credibility of functional food claims. Income and education level appear to be factors that influence adults' perception of functional food consumption. Middle-aged adults (45 to 65 years) tend to perceive functional foods as medications, while young adults (18 to 44 years) who consider nutritional quality important when choosing food for consumption tend to have a better perception of functional foods. The recognition of the benefits and the need for functional foods indicates that this market remains promising. However, measures to instill greater consumer confidence should be adopted.

Context: Lactic acid bacteria (LAB), which are found in many fermented foods, are known to produce antimicrobial compounds that play a vital role in food bio-preservation. Aims: To screen, identify, characterize, and determine the secondary metabolites of LAB isolated from Thai fermented foods that are beneficial against Escherichia coli and Staphylococcus aureus. Methods: The antimicrobial activity of cell free supernatant (CFS) was evaluated by agar well diffusion assay, and determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC). Bacterial strains were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and chemical compound was conducted by gas chromatography and mass spectrometry (GC-MS). Additionally, microbial dynamic and bile salt tolerance were assessed. Results: Seven of the 90 lactic acid isolates from Thai fermented foods, showed antibacterial activity against E. coli and S. aureus in the 12.00–16.00 mm inhibitory zone. It was identified that the bacteria were Lactobacillus pentosus, Lactobacillus farciminis, Lactobacillus brevis, and Lactobacillus plantarum. The best antibacterial activity was represented by LBST1861 strain, which also provided bile salt resistance at 0.3% for 24 hours and had MIC and MBC values of 12.5 mg/mL and 50.0 mg/mL against S. aureus and E. coli, respectively. Furthermore, the GC-MS discovered a total of 16 chemical compounds that may be used to limit microbial growth and has a potential to be employed as a bio-preservative. Conclusions: The most potent strain of LBST1861 strain against S. aureus and E. coli as L. plantarum, isolated from fermented foods in Thailand, generated significant bioactive chemicals that can be applied to promote food products.

Context: Given the elusive nature of Primary Amoebic Meningoencephalitis (PAM), caused by Naegleria fowleri, early detection is vital, yet challenging due to limited clinical indicators. This research leverages Indonesia’s rich biodiversity to explore novel sources of traditional medicine. Aims: To evaluate the potential compounds from Indonesian plants that possess antiamoebic and antifungal properties for inhibiting the N. fowleri CYP51 protein, crucial for cell integrity. Methods: Initially, 92 compounds were screened, and six were shortlisted following ADMETox evaluation. Subsequent steps encompassed QSAR analysis, molecular docking, and molecular dynamics simulations. Results: The QSAR analysis verified the activity potential of these six compounds, progressing them to molecular docking analysis. Among these, curcumenol from Curcuma longa emerged as a promising contender, displaying the lowest binding affinity at -9.2 kcal/mol, indicative of superior binding compared to other ligands. Molecular dynamics simulations underscored the stability of all compounds, with root mean square fluctuation (RMSF) values within 1-3 Å. Conclusions: Consequently, employing a comprehensive approach spanning ADMETox, QSAR, molecular docking, and dynamics simulations, curcumenol emerged as the prime candidate for inhibiting the N. fowleri CYP51 protein, suggesting its potential as a PAM therapeutic agent.

Context: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread, causing a global pandemic with diverse symptoms and increased risk of mortality. Various symptoms and comorbidities contribute to a higher likelihood of death in patients. Additionally, existing antiviral drugs have shown incomplete efficacy. Rafflesia sp. and Sapria sp. are parasitic plants with potential medical applications as anti-SARS-CoV-2 agents. Aims: To evaluate the bioactive compounds derived from Rafflesia sp. and Sapria sp. as dual inhibitors against SARS-CoV-2. Methods: Ligand samples were obtained from the PubChem database. Target proteins essential for SARS-CoV-2 entry were obtained from the RCSB PDB. The antiviral potential of the bioactive compounds was evaluated using the Pass Online webserver. The bioactivity and inhibitory potential of selected ligands were analyzed using the SwissADME and Molinspiration web servers. In addition, a specific docking method was performed using PyRx software to determine binding activity and molecular interactions. Results: Computational analysis revealed that leucoanthocyanidin, ellagic acid, and catechin functioned as dual inhibitors, targeting angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), furin, and cathepsin L for antiviral activity. However, valrubicin and diminazene, serving as control drugs for ACE2 and furin, respectively, demonstrated the most effective results through this mechanism. Further studies are required to validate these findings. Conclusions: The combination of bioactive compounds derived from Rafflesia sp. and Sapria sp. shows potential antiviral activity through a dual inhibitor mechanism involving leucoanthocyanidin, ellagic acid, and catechin, which target SARS-CoV-2 proteins, namely ACE2, TMPRSS2, furin, and cathepsin L.

ABSTRACT In this study, chemical changes during the development of acerola, cultivar Okinawa, were evaluated. The biochemical alterations during the development of the acerola cultivar Okinawa were evaluated, to determine the peroxidase activity (POD) and its thermal stability. Peroxidase (POD) has been considered the main enzyme responsible for the quality deterioration of fruits. Additionally, characteristics evaluated in this study can be important postharvest quality criteria for fruit processing, screening and breeding. Changes in physiological development were evaluated by the determination of moisture content, ascorbic acid, ashes, total and reducing sugars, pectin, chlorophyll a and b, amounts of anthocyanins, flavonols and total carotenoids. These biochemical determinations may serve as a basis for further improvement of techniques for predicting and modeling the optimal harvesting time. The values of chlorophylls, anthocyanins, flavonols, and carotenoids were higher in the peel rather than in the pulp (p < 0.05). Acerola presents high levels of ascorbic acid. The development of acerola (from opening of flowers to maturation of fruits) was of 25 days. The increase of the values for total and reducing sugars, anthocyanins, carotenoids, the solubilization of pectic acid and degradation of chlorophyll suggested that physiological maturation and harvest day occurred on days 19 and 23 after anthesis, respectively. The specific activity of POD was higher in the green stage. Maximum POD activity was observed at pH 6.5 and 45 ºC. Total inactivation was not achieved, suggesting the presence of isoenzymes with high thermal resistance. A loss of 50% of activity occurred after 4 minutes of treatment at 80 °C.

Phytophthora palmivora is a ubiquitous pathogen responsible for “dry branch” disease, causing significant economic losses in olive trees. Synthetic chemical fungicides are currently used for the control of P. palmivora. The general concern about the negative consequences of using synthetic products prioritizes the search for eco-friendly alternatives. In this context, plant extracts have emerged as an interesting and promising alternative for crop protection. This work studies the inhibitory activity of Larrea divaricata extract on P. palmivora mycelial growth, sporangium and zoospore production. The extract showed fungicidal activity against P. palmivora mycelial growth at concentrations over 150 mg mL-1. Specifically, the extract at 50 mg mL-1 completely suppressed the production of P. palmivora sporangia and zoospores. The alkaloid piperine in L. divaricata extract showed antimicrobial activity against P. palmivora mycelial growth. Extract effectiveness was also evaluated on olive trees in a greenhouse, showing 63% of disease reduction. These results support the use of L. divaricata extract as another environmentally friendly tool to be included in an integrated disease management program for dry branch disease caused by P. palmivora. Highlights: • L. divaricata extract showed an effective antimicrobial activity against P. palmivora. • Piperine was identified in the divaricata extract by HPLC/MWD. • The alkaloid piperine in divaricata extract showed antimicrobial activity on the mycelial growth of P. palmivora. • L. divaricata extract could be included in an integrated management programme for dry branch disease caused by P. palmivora.

The objective of this research was to evaluate the effects of dynamic high pressure at different processing temperatures, on the extraction of bioactive compounds from Peruvian purple corn cob. The purple corn cobs were ground and sieved to then be mixed with an acidified 20% ethanol-water solution (pH 2) in a 1:30 (w/v) ratio. The hydroalcoholic extracts were subjected to maceration and high dynamic pressure processes varying temperature (25 °C to 60 °C) and pressure (0.01 MPa - 60 MPa), and the bioactive compounds content and antioxidant activity were evaluated. The results showed that dynamic high pressure at 60 MPa / 45 °C was capable of obtaining extracts with bioactive compound contents and high antioxidant activity corresponding to those obtained using the conventional process of 2.5 hours / 65 °C. Seven different anthocyanins were identified by liquid chromatography in the extracts obtained by the dynamic high pressure, mainly cyanidin-3-glucoside, pelargonidin-3-glucoside and peonidin-3-glucoside, and their respective malonyl derivatives. The dynamic high pressure increased the extraction of anthocyanins by more than 108% and obtained them in one step, thus appearing as a new alternative, eco-friendly method for the extraction of bioactive compounds from plant tissues.

Abstract Lychee (Litchi chinensis Sonn.) is an exotic fruit from Asia, recently introduced in Europe. With the increase in the world production of this fruit, many by-products are wasted during industrial processing, including their peels and seeds. Considering the utilization of fruit peels as edible material as a new functional ingredient, this work aimed to assess the nutritional and phytochemical characterization, as well as the antioxidant activity of two lychee peel extracts (alcoholic and hydroalcoholic). Also, it was evaluated the scavenging capacity against reactive species (O2•-, H2O2, NO•) of the two extracts. The peels, at a nutritional level, showed high levels of carbohydrates and total energy (76.8 ± 1.0 g/100 g and 331.4 kcal/100 g, respectively) and low protein and lipid content. Total phenolic and flavonoid contents were higher in alcoholic extraction (1578 mg GAE/g and 55.1 mg CE/g, respectively). The antioxidant activity evaluated in vitro by DPPH and FRAP assays was also higher in the ethanolic extract, verifying a positive correlation with the extractive yield of the bioactive compounds. In general, the ethanolic extracts of lychee peels showed higher antioxidant capacity and the maximum scavenging activity against reactive oxygen (O2-•) and nitrogen species (NO•). The hydrogen peroxide scavenging activity observed in ethanolic extract (64 µg/mL) was like the values obtained in the positive controls (quercetin and ascorbic acid, 62 µg/mL, and 46 µg/mL, respectively). These preliminary results suggest this undervalued ingredient is a promising source of bioactive compounds with high biological potential for the development of new products as functional ingredient, always focusing on sustainability.

Abstract This work aimed to investigate the physicochemical and functional composition of bee pollen produced in different municipalities in the state of Rio Grande do Norte/Brazil (RN) and commercial samples produced in other places. To perform these experiments, samples of dehydrated bee pollen were crushed and subjected to physicochemical evaluation, instrumental color, fatty acid profile, evaluation of the presence of bioactive compounds [Total Phenolic Compounds (TPC), carotenoids] and antioxidant activity (reduction of DPPH radical). The pH of the pollen samples varied from 4.55 to 5.08, the protein from 19.01% to 29.71%, and the ash from 2.44% to 4.27%. All pollen samples showed a yellowish-red hue (a* and b* positive), and the pollen produced in Touros/RN/BR stood out with a lighter color (L* = 32.91). The pollen produced in Touros/RN/BR showed a higher (p < 0.05) percentage of saturated fatty acids (41.60%), whereas the pollen collected in Taipu/RN/BR had a higher percentage of monounsaturated fatty acids (7.44%). Pollen samples showed a low concentration of Total Carotenoid Content (TCC) (0.81 to 22.82 µg/g), as well as a high content of TPC (1.79 to 2.28 mg GAE/g) and antioxidant activity (1.40 to 5.70 µmol TE/g) when compared with the consulted literature. According to the obtained results, we could infer that pollen samples from different locations showed variations in physicochemical composition, coloration, fatty acid profile, TPC and antioxidant activity which reflects the geographic diversity inherent to the production sites.

Abstract Although the tocopherol concentration in Soybean Oil Deodorizer Distillate (SODD) regarding molecular distillation (MD) has been studied in the last 20 years, no scientific studies were published yet in international journals about the results of this technology when applied in an industrial scale. This research aimed to evaluate the tocopherols concentration through industrial patented MD technology in a vegetable oil refinery, using a phospholipase A2 enzymatic physical degumming process, packed dual temperature column deodorizer coupled to a double washer and a Tocoboost® patented technology. The results of the refinery production process from July 2019 to February 2020 could totalize over 81 days, comprising 243 samples and more than 1900 laboratory analysis. The input and output SODD samples were analysed by Gas Chromatography (GC), in which graphics of the automation system and mass balance of the refinery production ranges were also verified. The results showed that it is possible to achieve over five times Vitamin E concentration from non-modified soybean oil deodorizer distillate in a physic enzymatic phospholipase degumming process plant using a combination of advanced technology in order to separate high value-added molecules gained through studied hypotheses regarding vacuum, temperature, distiller models, backset flows, multiple stages of distillation, degradation and product recovery.

Abstract The use of unconventional edible plants in human food has grown more widespread in recent years, driven mainly by gourmet dishes. Evidence of the food properties of these species is still scarce. Aiming to obtain more in-depth knowledge of dahlia plants, this study aimed to evaluate the nutritional, bioactive, and antinutritional properties of the edible parts of purple dahlia (tuberous root and flower) to elucidate their value and safety as foods. The percent composition, caloric value, pH, Total Soluble Solids (TSS), Total Titratable Acidity (TTA), ratio of TSS to TTA, anthocyanins, carotenoids, vitamin C, total phenolics, tannins, nitrates, and minerals of the roots and flowers were analyzed. The inulin content in the root and the color of the flower were also evaluated. The results were reported as mean and standard deviation, and exploratory factor analysis was performed. Both parts of the dahlia had constituents that contribute to a good diet at concentrations like those in conventional vegetables, whereas the antinutritional components were also compatible with those of commonly consumed foods, with acceptable dietary values. Thus, it is evident that purple dahlia flowers and roots have potential for use in human food and can be considered good options to improve and diversify a healthy diet.

Abstract The objective of this work was to evaluate the physicochemical composition of juices from the 'Bordô' and 'BRS Cora' grapes grown in an organic production system in the Serra do Sudeste region, in Southern Brazil, as well as to compare the cultivar results with data from other producing regions and with the requirements of the Brazilian regulation for whole grape juice. The used grapes came from a commercial vineyard, especifically from the 2016-2017, 2017-2018, and 2018-2019 production cycles. The juices were prepared by the steam drag method and evaluated for their physicochemical composition. The fruit showed suitable values for the parameters necessary for grape juice production. In general, the juices showed bioactive compounds contents similar to those obtained in other regions. The 'Bordô' juices evaluated in the three production cycles showed significant differences for several variables, which were attributed to the influence of weather on grape maturity. The juices of 'BRS Cora' showed high-consistency qualitative results even under the very distinct meteorological conditions of the three production cycles. The juices comply with the current Brazilian regulation for whole grape juice, except for soluble solids, which are severely lowered by the used extraction technique.

Introduction: Snacks have become an important part of the daily diet, however, their nutritional profile could be improved by adding ingredients with health benefits. The aim of this work was to develop a functional dietetic snack using black carob flour and discarded blueberries, and to evaluate its chemical and functional composition. Methods: Three formulations were prepared using different proportions of wheat flour and black carob flour: 60:40; 50:50; and 40:60, respectively. Proximal chemical composition was determined by the procedures of AOAC methods and functional properties were also analyzed. Results: Three dietary snacks were obtained, which showed a caloric reduction of 20 - 23%. In all snacks, an increase in protein content of more than 60% was achieved and a higher total dietary fiber content (DS2) was observed compared to commercial snacks. All formulations showed a reduction in bioactive compound content during processing. Conclusions: The snacks showed functional properties, higher protein content and a reduced total caloric value compared to commercially available products. DS2 was the snack with the highest antioxidant activity and, according to fiber values, is a product with “high fiber content”. Despite the losses caused by the cooking process in the content of bioactive compounds, the antioxidant activity was greater than 50% in all the snacks produced.

The optimal process conditions of the advanced aqueous method for recovering oil and de-oiled meal from pumpkin seed kernels were: baking the kernels at 110 °C for 1 min, grinding them to pass through a sieve of 150 μm pore size, adding 1.60 ml brine to 10.00 g ground kernels, stirring for 30 min at 30 °C, centrifuging at 4000 r/min for 30 min and cold-pressing the residue from centrifugation. This method recovered > 94% oil. Its oil recovery rate was comparable to that of solvent extraction and higher than that of enzyme-assisted aqueous method or hot-pressing. It recovered edible oil with higher quality and level of coenzyme Q10, tocopherols, carotenoids, total phytosterols and squalene as compared to solvent extraction or hot-pressing and requirements of China’s national standard. It is superior to enzyme-assisted aqueous method or hot-pressing for recovering de-oiled meal which is suitable for making texturized protein.

Abstract Jambolan (Syzygium cumini (L.) Skeels) is an under-explored fruit rich in polyphenols, which are associated with health benefits, such as increasing resistance to oxidative stress, inflammatory processes and cardiovascular, and platelet functions. These polyphenols can be obtained by extraction, but an efficient standard method remains a challenge. In this context, this work evaluated the impact of different pretreatments on jambolans to obtain bioactive compounds by aqueous extraction. An Air Circulation Oven (ACO) and Lyophilization (LYO) were used as pretreatments. In addition, the influence of mass, temperature, cycle, and time parameters were studied in the extraction methods used: Percolated Solid-Liquid (PSL), Conventional Solid-Liquid (CSL), and solid-liquid assisted by ultrasound (USL). The extraction yield was from 7.3% (ACO) to 46.3% (LYO), both using the PSL method. In addition, eleven phenolic compounds and six anthocyanins were detected by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), in expressive amounts of catechin and cyaniding in the LYO sample and, these extracts showed higher concentrations of bioactive compounds. The CSL method was more efficient on ACO samples and PSL on LYO samples. LYO extracts showed higher concentrations of bioactive compounds. Therefore, the use of a drying pretreatment results in extracts with a high antioxidant potential for application in the food, cosmetic, and pharmaceutical markets.

Soymilk is well known for its health and nutritional benefits and is one of the best plant substitutes for cow milk. Soymilk is high in protein, low in cholesterol, lactose-free, and rich in polyunsaturated fatty acids. The bioactive compounds in soybean contribute to the beneficial effects of soymilk and are reported to exert various bioactivities. With the rising interest in health-conscious lifestyles, the development of soymilk with high nutritional quality is a critical task of the soymilk industry. Therefore, research on novel and advanced technologies is underway to develop soymilk with maximal nutritional quality. This review aims to present the recent findings on the beneficial effects of the bioactive compounds in soymilk and to introduce the latest technological advances that enhance the nutritional quality of soymilk, focusing on increasing the amount of nutrients and bioactive compounds, anti-nutrient removal, fortification with bioactive ingredients, and bio-enrichment.

Mango is the second most commercialized tropical fruit in the world, and Mexico is the major exporter. In terms of mango production, Manila´s variety represents a quarter of the total mangoes production in Mexico. However, the changes that occur on the phenolic compounds during the Manila mango ripening process are unknown. Quantitative analysis of the major phenolic compounds was conducted at different maturity stages, using several spectrophotometric measurements and by high-performance liquid chromatography (HPLC). At the late ripening stage was observed the biggest content in pulp and peel of total phenols (577 and 10547 mg EAG /100 g), flavonoids (95.33 and 537 mg EQ/100 g), and antioxidant capacity by DPPH (25 and 347 mmol TE/100 g). Some bioactive compounds achieve their highest values at optimal consumption ripening. Although they diminish when the fruit reaches a senescence appearance. This is the first study to prove that mangiferin by itself shows a higher correlation in antioxidant capacity compared to other phenolic compounds in mango peel, and this suggest that phenolic compounds may have an important role in the postharvest antioxidant metabolism in Manila mango fruit. On the other hand, the results show that the peel compared to the pulp contains higher amounts of total phenols, flavonoids, gallic acid, mangiferin and antioxidant capacity, so its use as an ingredient in the preparation of functional food products is recommended. More studies are needed to go in-depth in the changes of the content of phytochemicals during the ripening process in the peel and pulp mango, which ones could be caused by the hormones responsible for ripening in the fruit, such as ethylene, and bioavailability of these compounds at different stages of maturation.

Seaweed extracts are widely used in agriculture as plant growth biostimulants and constitute an ecological alternative to the excessive consumption of synthetic agrochemicals. These bioproducts are complex mixtures of several bioactive compounds such as plant growth regulators, polysaccharides, phenols, amino acids, sterols, betaines, vitamins, macro and microminerals. Its application in the production of different crops generates a wide spectrum of positive responses in the soil-plant system. However, the biostimulatory potential of these formulations has not been fully exploited due to the limited knowledge about their mechanisms of action in plants. This review aims to analyze the perspectives of seaweed as a source of bioactive extracts for the promotion of plant growth and crops protection. The main compounds with biological activity in seaweed extracts and the methods for their extraction are described. In addition, the hormetic effects of these bioproducts on seed germination, root-system development, rhizospheric microbiome, crop yield and quality, plant nutrient uptake and assimilation, plant metabolism and physiology are analyzed; as well as in the mitigation of abiotic stress and the management of pests and diseases. Finally, the predominant metabolites in Cuban seaweed extracts for the development of new bioproducts for agricultural purposes are described.

Background: The genus Randia L. (Rubiaceae) is native to Americas and highly distributed in tropical areas. Some Randia species are used in traditional medicine in some countries to treat diverse illnesses/symptoms of kidney, circulatory system, lungs, diabetes, cancer, inflammation, and against the bites/stings of snakes and other poisonous animals. Questions: What are the phytochemical compounds previously identified in Randia? What biological activities do they present? Data description: Twenty-eight studies on chemical composition and biological activities of Randia were reviewed. Species names were corroborated in Plants of the World Online and World Flora Online. The site and studied years: Studies of Randia of Americas from 1991 to 2021. Methods: Database reviewed were PubMed, Scopus, Scielo, BVS, DAOJ, Science Direct, Springer Link, Web of Science, and Google Scholar, employing the keywords Randia and its synonym Basanacantha. Results: Six species are the most studied (R. aculeata, R. echinocarpa, R. ferox, R. hebecarpa, R. matudae, and R. monantha). Ethnopharmacology information of 12 species was recovered. One hundred compounds in Randia have been identified (phenolic acids, terpenes, sterols, and others), and diverse biological activities reported in 24 studies (e.g., antimutagenic, antioxidant, and antivenom) have demonstrated for nine species. Conclusions: Biological activities found in some species of Randia support their traditional uses, but only the antivenom effect of Randia aculeata has been demonstrated. Randia species could be a source of bioactive compounds; however, knowledge must be expanded to demonstrate their traditional uses and contribute to the development of strategies for their preservation and rational use.

The ultrasound-assisted method was used to extract oil from the red mombin seed, mainly aiming to analyze yield. A multivariate analysis served to define optimized parameters (6.46 minutes and S/S ratio of 1:23.10 mass:volume) for ultrasound-assisted extraction (UAE) with the objective of maximizing yield, using the response surface methodology (RSM) and desirability graph with central variables and axial points determined by the central composite rotatable design (CCRD). In addition to the optimization of oil extraction, oil was chemically characterized in terms of antioxidant capacity and nutritional aspects to test the quality and chemical characteristics of red mombin seed oil extraction residue (cake). Analyses showed 32% unsaturated fatty acids, such as palmitoleic acid, linolelaidic acid, and α-linolenic acid, and the presence of phenolic compounds, especially catechin. High dietary fiber content and the presence of phenolic compounds, such as chlorogenic acid, vanillin, and gallic acid, were found in the cake, which allows the possibility of incorporating this material into food products.

Context: Marine microorganisms represent a promising source of bioactive molecules for biomedical applications. Increasing scientific literature is describing novel metabolites isolated from marine microbes with attractive pharmacological properties, such as anti-inflammatory, immunomodulatory, and anticancer. Aims: To reveal a background of the main marine microbial-derived products that have been isolated and characterized, including recent examples. The main mechanisms of action of these compounds in different models are also discussed. Methods: This research was structured based on a four phases design. 1) the identification of research questions, 2) selection of relevant studies, 3) filtering of studies based on inclusion and exclusion criteria, and 4) collection and organization of the data. For the web search, were used PubMed, Web of Science, Science Direct and ProQuest. For the selection and classification of the papers was used PRISMA software. Results: A wide variety of marine microbial metabolites with important pharmacological properties have been discovered and characterized so far. The main sources of these compounds are marine actinomycetes, bacilli, fungi from Aspergillus and Penicillium genus, microalgae, and some marine symbiotic bacteria and fungi. Most of these metabolites exhibit cytotoxic, pro-apoptotic, anticancer, anti-inflammatory, and immunomodulatory activities. Complex structural moieties, such as multiple aromatic rings and heteroatoms, seem to be related to these properties. The mechanisms of action of most of these molecules target apoptosis-related proteins, enzymes, transcription factors, DNA binding proteins and some cell surface receptors. Conclusions: The marine environment offers an efficient and attractive way to obtain novel natural products. Marine microorganisms are a prolific source of new molecules and extracts with therapeutic potential in the treatment of chronic inflammatory diseases. They represent and ecofriendly and feasible option to obtain drug candidates with multiple mechanisms of action and important biomedical applications.

The tomato processing industry generates a large amount of waste, usually composed of peels and seeds. It is known that the tomato is rich in bioactive compounds, mainly carotenoids (lycopene and β-carotene), however, studies on the functionality of its residues are scarce. Thus, these residues are discarded without the knowledge of possible antioxidant potential. The objective of this work was to determine the stability of bioactive in soybean oil added to the extract of agroindustrial tomato waste under heating. The extract obtained with ethyl alcohol at the ratio of 1:5 (w/v) was initially analyzed for total phenolic compounds, lycopene, β-carotene, and antioxidant activity using the DPPH and β- carotene / linoleic acid. Subsequently, the extract and the synthetic antioxidants, butylhydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ) were applied in soybean oil, and the treatments subjected to heating in Rancimat (180ºC/5 h). The thermoxidated samples were analyzed for the composition of tocopherols by high-performance liquid chromatography (HPLC), phytosterols, and fatty acid profile by gas chromatography (GC). The extract of agro-industrial tomato residues showed moderate results concerning its antioxidant activity, 68% by the DPPH method, and 26.2% of auto-oxidation of the β-carotene / linoleic acid system. The synthetic antioxidant TBHQ had a greater protective effect on soybean oil when compared to the control, providing greater retention of phytosterols (85%) and tocopherols (93%). Tomato extract can be used as a natural antioxidant in industrialized oils, but with less effectiveness than the antioxidant TBHQ.

In this work, the peels of ripe banana (Musa sp.), variety “Prata”, were used to prepare a flour, which was evaluated for its physicochemical characteristics and antioxidant activity. The characterization of its chemical profile was performed by a fast and simple method: paper spray-mass spectrometry. This flour had high carbohydrate content and total dietary fiber, and significant amount of total phenolic compounds with antioxidant activity. In addition, this flour exhibited higher water-holding, oil-holding and swelling capacities than those of fruit by-products reported in the literature. Fourier-transform infrared spectroscopy confirmed the presence of functional groups of cellulose, hemicellulose, and lignin, constituents of insoluble dietary fiber. By the paper spray-mass spectrometry technique, it was possible to suggest the presence of 26 substances such as organic acids, phenolic acids, flavonoids, sugars, quinones, and terpenoids. Thus, this study demonstrated that the ripe banana peel flour has potential application as an ingredient for functional foods.

This study was conducted to analyze bioactive compounds and the in vitro antioxidant capacity of tea infusions from whole and ground medicinal herbs, including cardamom, cinnamon, clove, nigella, and ginger. Samples of whole medicinal herbs were ground to pass through a 40 mesh (425 µm) sieve, and hot water (95 °C) infusions of whole and ground herb parts were examined. Higher bioactive compounds such as phenolic compounds and flavonoids were found in the ground tea infusions. Among the medicinal herb samples, clove tea infusion showed the highest total phenol content, total flavonoid content, ABTS, and DPPH radical scavenging capacity in an average of whole and ground tea infusions. Higher β-carotene and anthocyanin content were also found in the ground nigella and cinnamon medicinal herb tea infusion than whole infusions, respectively. Therefore, ground tea infusions may be more effective in maximizing bioactive compounds and antioxidant capacity than whole infusions.

Tannat skin from grape pomace is a sustainable source of bioactive compounds and dietary fiber. In previous studies we have seen that it has antioxidant, antidiabetic, anti-obesity and anti-inflammatory activity, with the potential to prevent the development of chronic diseases. In this work, the encapsulation of bioactive compounds of an ethanolic extract derived from Tannat grape skin by microparticles of whey protein isolate (without and with enzymatic hydrolysis) and inulin (3:1) is proposed for its application in yogurt as a potential functional food. Spray drying encapsulation was carried out using a tabletop spray dryer (inlet air temperature: 100-140°C, Flow: 600 L/h). The encapsulation efficiency was higher for the encapsulant without hydrolysis (29.7%). Total polyphenol content was determined by Folin-Ciocalteau, and the antioxidant capacity by ABTS and ORAC-FL, obtaining a polyphenols content and antioxidant capacity increase with the addition of extract:encapsulant (1:1) (p

The present study evaluates the contents in bioactive compounds, antioxidant activity, oil content and fatty acid composition of Capparis spinosa seeds. Samples were collected from 5 different habitats (AH: Ahar; KU: Kurdistan; U1, U2 and U3: Urmia) in Iran. The oil content in the seeds ranged from 16 to 27%. The predominant fatty acid was linoleic acid (45-50%) followed by oleic acid (30-39%), palmitic acid (2-8%) and stearic acid (2-3%). Total phenolic content (TPC) varied from 16.3 to 24.2 mg GAE/ g DW; total flavonoid content (TFC) ranged from 1.48 to 3.05 mg QE/g DW; and the antioxidant activity (DPPH assay) of the seeds was between 35 and 63%. The compounds obtained from different genotypes of C. spinosa seeds had different compositions, great antioxidant capacity and unsaturated fatty acids, and therefore could be a prospective source of natural bioactive molecules for the food and health industry.

A commercial enzyme preparation consisting of pectolytic, cellulotic and hemicellulotic enzymes was applied to the oil extraction by cold pressing from apricot kernel, flaxseed and grape seed. The effects of enzyme pre-treatment varied depending on the different oil seed used as raw material. Although the increase in free fatty acidity can be considered as a negative effect (from 0.37 to 0.52), the decrease in peroxide number and p-anisidine values, increase in oil yield (22.75%), higher levels of total carotenoids and tocopherols, as well as a remarkable increase in phenolic content (x1.68) and radical scavenging effect (including hydrophilic and lipophilic-induced and total antioxidant capacity) showed that the use of enzyme application in the cold pressing of apricot kernel oil would be beneficial. Many of these positive results could not be achieved in the pressing of flaxseed or grape seed oils under the same conditions. A high negative correlation (r=-92.2) was found between p-anisidine value and δ-tocopherol for grapeseed oil. Hydrophilic and lipophilic antioxidant capacity, total phenolics, and total carotenoids negatively correlated well (r values above 80) with peroxide values for apricot seed oil. Correlation results showed that carotenoids play an important role in the oxidative stability of the oils, where it was much more evident for apricot seed oil (r=-97.5).

ABSTRACT: Brazilian Cerrado fruits have a great diversity of bioactive compounds, with significant relevance for fresh consumption and industrial processing. The uvaia (Eugenia pyriformis Cambess) stands out among these fruits, seeing that this species contains high phenolic compounds and ascorbic acid levels. However, its technological and nutritional potential is still little explored industrially. This research aimed to develop three uvaia nectar formulations and to evaluate its stability during 90 days of storage. The formulations were made with 30%, 40%, and 50% of uvaia pulp, corresponding to F1, F2, and F3 formulations, respectively, which were characterized for pH, total titratable acidity, soluble solids, ascorbic acid, and total phenolics during 0, 30, 60, and 90 days of storage at room temperature. A significant pH reduction was observed after 30 days of storage for all nectars. The acidity and ascorbic acid levels increased in proportion to the addition in the pulp concentration of the formulations. The ascorbic acid content reduced over the storage period and remained higher for the formulations F2 and F3. The concentration of phenolic compounds was higher in the nectar containing relevant levels of uvaia pulp concentration, which increased throughout 30 days of storage, however, with a decrease after this period. The formulations containing 40% and 50% of uvaia pulp showed higher concentration and better stability of bioactive compounds. Uvaia can be considered an effective raw material for the development of a novel beverage with important nutritional and bioactive properties.

Background. Successful immunomodulatory effects of yeast structural and secretory bioactive compounds have been demonstrated on several animal species. Likewise, studies have shown the ability of Debaryomyces hansenii to stimulate both immune and antioxidant responses on fish, crustaceans, mollusks, and recently, goats. Objective. To analyze the immunostimulant activity of lysates derived from five strains of the marine yeast D. hansenii in goat peripheral blood leukocytes using in vitro assays. Methodology. For this purpose, leukocytes from caprine peripheral blood were isolated and incubated (24 h) with lysate-derived D. hansenii strains and Zymosan (positive control). Results. The results revealed that these strains significantly increased cell immune parameters, such as phagocytic capacity, reactive oxygen species (respiratory burst) and nitric oxide production, and myeloperoxidase activity. Antioxidant enzyme assays in leukocytes stimulated with lysate-derived D. hansenii demonstrated significant increases in superoxide dismutase and catalase activities. Implications. This in vitro study using peripheral blood leukocytes suggest that lysates derived from marine yeast D. hansenii strains could be considered immunostimulants for goats through their ability to stimulate the non-specific immune parameters and the antioxidant immune mechanism, without affecting leukocyte viability. Conclusion. In particular, the results indicated that D. hansenii BCS001 was able to modulate the immune parameters in goat immune cells. Therefore, additional in vivo immunostimulatory assays deserve further research, including other species of economic interest in food production.

Abstract Ora-pro-nobis (OPN) (Pereskia aculeata Miller) is a non-conventional edible plant rich in protein, fibres and minerals. The innovation of this work is based on the sustainability production of the aqueous extract (green solvent) containing iron and bioactive compounds as well as employing a native plant from Brazil. A screening of phytochemicals components, phenolic compounds, flavonoids and tannins contents were performed using OPN extract. The antioxidant activity of the OPN aqueous extract was determined by three different assays as following: 2,2-diphenyl-1-picrylhydrazyl (DPPH•); Ferric Reducing Antioxidant Power (FRAP); and Oxygen Radical Absorbance Capacity (ORAC). The OPN dried leaves presented high protein and minerals contents. Indeed, the Fourier-Transform Infrared Spectroscopy (FTIR Spectroscopy) analysis performed in OPN aqueous extract confirmed the appearance of representative functional groups of the bioactive compounds. Overall the results suggested that it is possible to use simple aqueous solvent to produce OPN extract rich in iron, bioactive compounds and within antioxidant activity that could be potentially used as functional food ingredient.

ABSTRACT The present work aimed to evaluate the contents of total phenolic compounds, total flavonoids and antioxidant activity, as well as to identify and quantify phenolic acids before and after simulated gastrointestinal digestion in vitro, in raw and cooked cowpea grains, of biofortified cultivars BRS Aracê and BRS Tumucumaque. The raw grains were analyzed as flour and the cooked grains were analyzed after maceration, before and after cooking and in the stages of the digestive process. The contents of total phenolic compounds were analyzed by the spectrophotometric method, using the reagent Folin-Ciocalteu, and total flavonoids using a spectrophotometric method with quercetin as standard. The antioxidant activity was evaluated using the free radical capture method ABTS (2,2'-azino-bis (3-ethylbenzothiazolino-6-sulfonic acid) and FRAP (Ferric Reducing Antioxidant Power). Eight phenolic acids were investigated, and the identification and quantification was performed by high performance liquid chromatography (HPLC), the content of total phenolic compounds and the antioxidant activity were reduced during cooking, but increased with simulated digestion in vitro, due to the release of bound forms. action of digestive enzymes, there was a difference in the behavior of the raw and cooked cultivars.The phenolic acids suffered degradation under gastrointestinal conditions, but the cultivars analyzed maintained compounds with relevant bioactivity (raw grain - gallic, caffeic and p-cumáric acids; cooked grain - acids and caffeine) and antioxidant activity, which can help protect against chronic non-communicable diseases, demonstrating that cowpea is a common food bioaccessible natural antioxidants.

Fucoxanthin was the carotenoid studied from the marine microalga Isochrysis galbana for its importance in preventing obesity and diabetes. In this manner, seven solvents were used to fucoxanthin extraction, highlighting methanol and ethanol with 6.282 and 4.187 mg/g, respectively. However, petroleum ether and n-hexane were the worst solvents for fucoxanthin extraction, obtaining approximately 12-folds less content. Extraction time was another relevant parameter in improve fucoxanthin extraction where 10 min was the best time reaching 7.464 mg/g under 100% ethanol. Finally, we propose the use of I. galbana as natural source of fucoxanthin, a bioactive compound useful for food industry.

The study investigated the influence of spray- and freeze-drying on the bio-oil powder quality. The powders were characterized by process efficiency, structure, colour, oil and bioactive compounds contents, fatty acid composition and oxidative stability. Cold-pressed bio-oils of evening primrose, borage and blueweed were homogenized with wall materials and water followed by spray-drying at 130°C inlet air temperature or freeze-drying at −56°C. It was shown that lower encapsulation efficiency of freeze-drying process correlated with higher surface oil content. The content of bioactive compounds was more affected by the processes than the fatty acid composition regardless of the method of drying. The powders produced by freeze-drying had a higher tocopherol content than those produced by spray-drying. Both spray- and freeze-drying significantly contributed to the changes of sterols concentration, but the changes were greater for bio-oil powders obtained by spray-drying.

The addition of red cactus pear peel and its mucilage on color, bioactive compounds and antioxidant capacity (AC) of yogurt was investigated. Peel and its mucilage were dried and used for the formulation of different yogurt blends following a constrained mixture design. Color, total phenolic compounds, total flavonoids, total betalains and AC were evaluated in formulated yogurts. An optimization process was conducted thereafter, and the bioactive compounds and AC of this yogurt were evaluated before and after gastrointestinal simulation. Results indicated that cactus pear peel and its mucilage give a nice magenta color to the yogurts, and also increase the bioactive compounds and AC of them. Optimization procedure indicates that yogurt formulated with 5.5% of cactus pear peel and 7.5% of mucilage displays the highest bioactive compounds and AC; moreover, they were significantly increased (p < 0.05) by the simulated gastrointestinal process.

The aim of this study was to determine the level of antioxidants in the seeds of wheat, lentil, pea, radish, broccoli, alfalfa, sunflower and amaranth germinated for 24, 72 and 120 hours. Following germination, the highest increases in the total phenols corresponded to 5.39, 4.62 and 2.20 times in broccoli, radish and alfalfa seeds respectively. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was higher after germination for 120 hours in all the seed types as compared with the non-germinated seeds. In radish, sunflower and alfalfa seeds, the ability to scavenge ABTS increased by 5.19, 1.34 and 2.90 times respectively, after germination. According to the different changes in the amounts of tocopherol isomers in germinating seeds can be summarized, that the α-tocopherol content increased and that of β-, γ-, δ-tocopherol decreased irrespective of the species, therefore suitable for germination time and seeds variety allows having products with a high nutritional and antioxidant properties.

The objective of this work was to identify and evaluate the bioactive compounds, as well as the antioxidant potential, present in mature fruits of cambuizeiro (Myrcia multiflora) from the Chapada do Araripe, Brazil. In the fruits were evaluated: vitamin C, total anthocyanins, yellow flavonoids, total polyphenols, total antioxidant activity by the FRAP, DPPH and ABTS methods, and the antioxidant superoxide dismutase (SOD) and catalase (CAT) activity. Cambui had an average vitamin C content of 56.40 mg/100 g, 2869.44 mg/100 g total anthocyanins 2858.50 mg/100 g yellow flavonoids and 1007.64 mg EAG/100 g of total polyphenols. While the total antioxidant activity according to FRAP method was 123.94 μM ferrous sulphate/g, DPPH was 1805.66 g/g EC50, and ABTS was 32.62 μM trolox/g. The enzymatic activity of SOD was 846,203 AU/g, while CAT did not read. These results indicate that the mature fruits of cambuizeiro present high antioxidant potential, corroborating to the idea that their consumption may result in benefits to human health.