1. A simplified Iohexol-based method to measure renal function in sheep models of renal disease
- Author
-
Carolina Mas-Sanmartin, Antonio Gonzalez-Bulnes, Laura Díaz-Martín, Alejandro Jiménez-Sosa, Flavio Gaspari, Ana Rodríguez-Rodríguez, Alberto Ortiz, Trond Jenssen, Paula Martinez-Ros, Esteban Porrini, Anders Åsberg, Sergio Luis-Lima, UCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Producción Científica UCH 2020, Luis-Lima, Sergio, Rodríguez-Rodríguez, Ana Elena, Porrini, Esteban, Ortiz, Alberto, Gaspari, Flavio, Diaz-Martin, Laura, Åsberg, Anders, Jenssen, Trond, Jiménez-Sosa, Alejandro, Martinez-Ros, Paula, and González-Bulnes, A.
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Pruebas de función renal ,Concordance ,Iohexol - Efectos fisiológicos ,030232 urology & nephrology ,Urology ,Coverage probability ,Renal function ,Disease ,iohexol plasma clearance ,Biology ,urologic and male genital diseases ,Measure (mathematics) ,General Biochemistry, Genetics and Molecular Biology ,Article ,Sheep - Urinary organs ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,sheep model ,medicine ,Ovejas - Aparato urinario ,lcsh:QH301-705.5 ,Lohexol plasma clearance ,Kidney ,General Immunology and Microbiology ,urogenital system ,renal function ,Kidney function tests ,Iohexol - Physiological effect ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,Iohexol ,General Agricultural and Biological Sciences ,medicine.drug - Abstract
Sheep are highly adequate models for human renal diseases because of their many similarities in the histology and physiology of kidney and pathogenesis of kidney diseases. However, the lack of a simple method to measure glomerular filtration rate (GFR) limits its use as a model of renal diseases. Hence, we aimed to develop a simple method to measure GFR based on the plasma clearance of iohexol by assessing different pharmacokinetic models: (a) CL2: two-compartment (samples from 15 to 420 min, reference method), (b) CL1: one-compartment (samples from 60 to 420 min), (c) CLlf: CL1 adjusted by a correction formula and (d) SM: simplified CL2 (15 to 300 min). Specific statistics of agreement were used to test the models against CL2. The agreement between CL1 and CL2 was low, but both CL1f and SM showed excellent agreement with CL2, as indicated by a total deviation index of ~5&ndash, 6%, a concordance correlation of 0.98&ndash, 0.99% and a coverage probability of 99&ndash, 100%, respectively. Hence, the SM approach is preferable due to a reduced number of samples and shorter duration of the procedure, two points that improve animal management and welfare.
- Published
- 2020