1. Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino
- Author
-
Jaime López M, Juan Carlos Roa S, Pablo Guzmán G, Carmen Gloria Ili G, José Manuel Ojeda F., Italo Capurro V, Patricia García M, Priscilla Brebi M, and Eugenio Suárez P
- Subjects
Hpv genotypes ,Gynecology ,medicine.medical_specialty ,Pathology ,Uterine cervical cancer ,Formalin fixed paraffin embedded ,biology ,Genotype ,business.industry ,Alpha papilloma virus ,General Medicine ,medicine.disease ,biology.organism_classification ,Squamous intraepithelial lesion ,Risk area ,Medicine ,Papillomaviridae ,business ,Viral genotype ,Uterine Cervical neoplasms - Abstract
The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.
- Published
- 2010