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Start Over Search Limiters Available in Library Collection Topic cryptosporidium Language spanish; castilian
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1. Ocorrência de infecção Cryptosporidium spp. em peixe-boi marinho (Trichechus manatus) Occurrence of Cryptosporidium spp. infection in antillean manatee (Trichechus manatus)

Author

João Carlos Gomes Borges, Leucio Câmara Alves, Jociery Einhardt Vergara-Parente, Maria Aparecida da Glória Faustino, and Erilane de Castro Lima Machado

Subjects
Cryptosporidium, região Nordeste, mamíferos aquáticos, sirênios, Northeastern Region, aquatic mammals, sirenians, Animal culture, SF1-1100
Abstract

A criptosporidiose constitui-se como uma zoonose que pode afetar o homem e uma ampla variedade de animais domésticos e silvestres, principalmente indivíduos imunodeficientes. O objetivo desse trabalho foi registrar a ocorrência de infecção por Cryptosporidium em peixe-boi marinho. Após ser constatada a mudança de comportamento de um peixe-boi marinho mantido nos oceanários do Centro Mamíferos Aquáticos, ICMBio - FMA, animal foi submetido à exame clínico e, posteriormente, à coleta de amostra fecal. As amostras fecais foram analisadas pela técnica de Kinyoun, teste de imunofluorescência direta e pelo corante 4'.6'-Diamidino-2-Phenilindole (DAPI). No exame clínico, o animal apresentou sinais de desconforto abdominal. Os resultados obtidos nas análises de microscopia de luz e fluorescente revelaram a presença de oocistos de Cryptosporidium nas fezes desse peixe-boi.Cryptosporidiosis is a zoonosis which can affect man and a wide range of domestic and wild animals, mainly immunodeficient individuals. The objective of this paper was reported the occurrence of a Cryptosporidium infection in Antillean manatee. After an unusual behavior of an Antillean manatee kept in captivity at the Centro Mamíferos Aquáticos, ICMBio - FMA, clinical examination and posterior fecal sampling was performed. Fecal samples were examined by the Kinyoun technique, Direct Immunofluorescence Test and also examined by 4'.6'-Diamidino-2-Phenylindole (DAPI) staining. At the clinical examination, the animal showed signs of abdominal pain. The results obtained by light and fluorescence microscopy analysis showed the presence of Cryptosporidium spp. oocyst in feces of this manatee.

Published
2009
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2. Cyclospora cayetanensis: Presentación de 20 casos

Author

Isabel Martínez Silva, Lucía Ayllón Valdés, and Xiomara Benítez Padrón

Subjects
CRYPTOSPORIDIUM, PROTOZOAN INFECTIONS, lcsh:RJ1-570, lcsh:Pediatrics, CRYPTOSPORIDIUM, PROTOZOAN INFECTIONS, INFECCIONES POR PROTOZOARIOS, DIARREA INFANTIL, DIARRHEA, INFANTILE
Abstract

Se presentan 20 pacientes (0,2 %) de un total de 7 956 que acudieron de enero de 2000 a julio de 2001 a la Consulta de Parasitología del Hospital Pediátrico Universitario "William Soler" con un cuadro de diarreas acuosas y otras manifestaciones clínicas, y donde se aisló en muestras de heces frescas el protozoario Cyclospora cayetanensis. Las diarreas estuvieron presentes en 17 enfermos (85 %), los vómitos y la anorexia en 9 pacientes (45 %), el dolor abdominal en 7 (35 %), el decaimiento y la fiebre se evidenciaron en 3 pacientes (15 %). El estado nutricional de estos niños fue normal. El Cyclospora cayetanensis es un parásito que puede observarse en edades pediátricas y uno más que se tendrá en cuenta en la etiología de las diarreas acuosas.The paper presents a report of 20 patients (0,2%) selected from 7956 patients who went to the Parasitology Department of "William Soler" University Pediatric Hospital with watery diarrheas and other clinical manifestations from January 2000 to July 2001. Protozoon Cyclospora cayetanensis was isolated in samples of fresh feces from the mentioned cases. Diarrheas affected 17 patients (85%), vomits and anorexia were present in 9 patients (45%), abdominal pain in 7 (35%), and fatigue and fever in 3 patients (15%) The nutritional condition of these children was normal. Cyclospora cayetanensis is a parasite that may be observed in pediatric ages and an additional one to be taken into account in the etiology of watery diarrheas.

Published
2002

3. Procesamiento de muestras fecales en el estudio de Cryptosporidium sp. mediante PCR

Author

Pérez-Cordón, Gregorio, Rosales-Lombardo, M. José, and Sánchez-Moreno, Manuel

Subjects
PCR, purification, purificación, Criptosporidiosis, ADN, Cryptosporidium, oocysts, DNA, ooquistes
Abstract

The present paper describes a protocol applied to fecal samples of cattle for the isolation and purification of Cryptosporidium sp. oocysts and the possible use of the same ones for genetic studies by means of PCR., El presente trabajo describe un protocolo aplicado a muestras de heces de ganado bovino para el aislamiento y purificación de ooquistes de Cryptosporidium sp. y el eventual uso de los mismos para estudios genéticos mediante PCR.

Published
2005

4. Procesamiento de muestras fecales en el estudio de Cryptosporidium sp. mediante PCR

Author

Gregorio Pérez-Cordón, M. José Rosales-Lombardo, and Manuel Sánchez-Moreno

Subjects
biology, purification, Criptosporidiosis, ADN, Cryptosporidium, oocysts, DNA, biology.organism_classification, Molecular biology, ooquistes, PCR, lcsh:Biology (General), purificación, lcsh:Q, lcsh:Science, General Agricultural and Biological Sciences, lcsh:QH301-705.5
Abstract

El presente trabajo describe un protocolo aplicado a muestras de heces de ganado bovino para el aislamiento y purificación de ooquistes de Cryptosporidium sp. y el eventual uso de los mismos para estudios genéticos mediante PCR. The present paper describes a protocol applied to fecal samples of cattle for the isolation and purification of Cryptosporidium sp. oocysts and the possible use of the same ones for genetic studies by means of PCR.

Published
2005