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For a successful seed production, it is necessary to know the size, i.e. 1000-seed weight, since it affects the sowing rate, plant density over the vegetative period as well as the seed yield per unit area. The study was conducted in field conditions, on plots where seed production of sunflower hybrids parental components was organized. Seed production was based at three different localities. Observation was conducted during the course of three years. The study was performed on 18 different genotypes. Considering the total number of the observed genotypes, 10 genotypes represented lines based on CMS, while the remaining 8 genotypes represented restorer lines. The study showed that 1000-seed weight was higher with CMS based lines than with restorer lines, which was expected considering the branching of the restorer lines. Sunflower 1000-seed weight depended on the year of observation and the observed genotype. Year had a significant effect on 1000-seed weight.