Search

Aim: To assess influence of toll-like receptors (TLR) ligands on the production of proinflammatory cytokine (TNFalpha) by peripheral blood mononuclear cells in patients with acute myocardial infarction (AMI)., Materials and Methods: Peripheral blood mononuclear cells (PBMC) obtained from 13 patients with AMI on 1st and 14th day and from 17 healthy donors were stimulated by peptidoglycan, poly(I:C), lypopolysacchide, zimozan, flagellin and CpG oligodeoxynucleotides, which are ligands of TLR1/2, TLR3, TLR4, TLR2/6, TLR5 and TLR9 respectively. Spontaneous and induced by ligands production of TNFalpha was evaluated in supernatants of PBMC., Results: Increased spontaneous production of TNFalpha by PBMC in patients with AIM was revealed on 1st day of the disease. Ligands of TLR2/6 and TLR4 demonstrated marked stimulatory effect on the production of TNFalpha by PBMC in patients with AMI compared with group of healthy subjects., Conclusion: Increased production of TNFalpha by PBMC in patients with AMI indicates the activation of TLR2 and TLR4 on PBMC. Hyperactivation of TLRs during acute pathologic conditions results in excessive production of proinflammatory cytokines, specifically TNFalpha, and can induce damage of cells and tissues involved in acute pathologic process.

The study was concerned with the effect of human ceruloplasmin on blastogenic reaction of lymphocytes (BRL) and cyclic nucleotide level in murine organs in the course of Lewis lung carcinoma development. Both ceruloplasmin and an immunomodulator--zymosan--were found to raise the level of cAMP and to lower that of cGMP in the immunocompetent organs (thymus and spleen) of tumor-bearing mice at all stages of the investigation. The results point to an immunomodulating action of human ceruloplasmin involved in tumor growth and the role played by cyclic nucleotides in the process.

The relationship between regeneration of the liver of (CBA X C57BL)F1 mice and the degree of its mononuclear infiltration was studied. Resection of the 2/3 of the liver mass was performed 1 and 5 days and 2 months after zymosan-induced mononuclear infiltration. The synthesis of DNA and mitotic activity of hepatocytes in the liver with infiltrations was higher as compared with control. The rate of liver regeneration was dependent on the scope of the mononuclear infiltration, reaching a maximum provided liver resection was made 5 days after injection of zymosan granules.