Search

Your search keyword '"Sparkes, M."' showing total 1 results
Start Over Author "Sparkes, M." Language russian
1 results on '"Sparkes, M."'

1. [Reactions of decarboxylated and side transamination during interaction of glutamate decarboxylase from Escherichia coli with substrate analogs, modified through C3 and C4 atoms].

Author

Khristoforov RR, Sukhareva BS, Dixon HB, Sparkes MJ, Krasnov VP, Bukrina IM, and Grishakov AN

Subjects
Amination, Carboxylic Acids chemistry, Circular Dichroism, Glutamate Decarboxylase metabolism, Pyridoxal Phosphate chemistry, Pyridoxamine analogs & derivatives, Pyridoxamine chemistry, Stereoisomerism, Substrate Specificity, Carbon chemistry, Escherichia coli enzymology, Glutamate Decarboxylase chemistry
Abstract

The interaction of glutamate decarboxylase with the aspartate and glutamate analogues modified at C3 and C4 was studied. 3-Arsonoalanine, 3-phosphonoalanine, 2-amino-4-arsonobutyric acid, 2-amino-4-phosphonobutyric acid, a mixture of diastereoisomers of 4-(methylthio) glutamic acid and erythro-4-(methylthio) glutamic acid were shown to be poor substrates for the enzyme. Their decarboxylation was accompanied by transamination of the coenzyme (PLP) to pyridoxamine phosphate (PMP) which reversibly inactivated the enzyme. With arsonoalanine only part of PLP was converted into PMP and another part irreversibly formed a complex. 4-(Methylsulfonyl)-L-glutamic and 4-[(phenyl)(hydroxy)phosphoryl]-L-glutamic acids did not react with the glutamate decarboxylase.

Published
1996

Catalog

Books, media, physical & digital resources
See catalog results