1. [Optimal conditions for determing ATP of microbial origin].
- Author
-
Imshenetskiĭ AA, Murzakov BG, Blokhin VA, Lysenko SV, and Lemenshko VV
- Subjects
- Buffers, Firefly Luciferin, Freezing, Luciferases, Methods, Sarcina analysis, Adenosine Triphosphate analysis
- Abstract
Endogenous luminescence of the luciferin-luciferase extract from firely tails was studied in determing low concentrations of ATP. The optimum concentration of the the extract, corresponding to the minumum effect of endogenous luminescence, was found in the reaction medium. The solution of luciferin-luciferase was unstable at room temperature and upon dilution. The activity of luciferin-luciferse solutions did not change after freezing in liquid nitrogen with following thawing. The content of ATP was assayed in the cells of Sarcina flava disintegrated with ultrasound.
- Published
- 1977