1. [Characteristics of migration of adipose tissue derived mesenchymal stromal cells after co-cultivation with activated monocytes in vitro].
- Author
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Grigor'eva OA, Korovina IV, Gogia BSh, and Sysoeva VIu
- Subjects
- Adipose Tissue cytology, Adipose Tissue metabolism, Adult, Becaplermin, Cell Differentiation, Cell Line, Cells, Cultured, Coculture Techniques, Extracellular Matrix metabolism, Gene Expression, Humans, Integrin alpha Chains genetics, Integrin alpha Chains metabolism, Integrin beta Chains genetics, Integrin beta Chains metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Middle Aged, Monocytes cytology, Monocytes metabolism, Protein Binding, Receptor, Platelet-Derived Growth Factor beta agonists, Receptor, Platelet-Derived Growth Factor beta metabolism, Signal Transduction, Tetradecanoylphorbol Acetate pharmacology, Chemotaxis drug effects, Extracellular Matrix genetics, Mesenchymal Stem Cells drug effects, Monocytes drug effects, Proto-Oncogene Proteins c-sis pharmacology, Receptor, Platelet-Derived Growth Factor beta genetics
- Abstract
Mesenchymal stromal cells (MSC) are considered to be promising tool of regenerative medicine. Migration of MSC toward damaged inflammatory site is essential for physiological tissue reparation. Therefore we studied modifications of migratory features of adipose tissue derived MSC (AT-MSC) after co-cultivation with activated monocytes derived from THP-1 cell line. As a result, we have observed an increased migration rate of AT-MSC in vitro in the absence of chemoattractant gradient as well as toward the gradient of PDGF BB (platelet-derived growth factor BB), which is well known chemoattractant for the cells of mesenchymal origin. Furthermore, the rate of directional AT-MSC migration through fibronectin was also increased. We have established that signaling from PDGFRβ which is activated through binding of integrin receptors with extracellular matrix may be possible way to stimulate cellular migration under simulated inflammatory conditions.
- Published
- 2014