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Takayasu arteritis is well-known rare form of large vessel vasculitis. Pulmonary involvement is observed in 50% of cases. However, pulmonary hypertension is a rare complication of pulmonary artery stenosis. In this article we present a rare clinical case of Takayasus arteritis with typical stenosis of subclavian, carotid arteries, and involvement of pulmonary arteries with development of severe pulmonary hypertension. The article also contains discussion of current methods of diagnosis and treatment of pulmonary artery stenosis in Takayasus arteritis with special focus on effectiveness and complications of angioplasty and surgery.

The authors conducted histological, immunohistochemical, and morphometric studies of 47 endomyocardial biopsies and 33 hearts with a high morphological criterion for the diagnosis of arrhythmogenic right ventricular dysplasia after F.I. Marcus et al. (2010), as well as intravital and postmortem cardiac magnetic resonance imaging (MRI). The average residual cardiomyocyte area was 39.7 +/- 12.8%, and the average cardiomyocyte diameter was 10.4 +/- 1.5 microm. Muscle fiber atrophy, lipomatosis, and fibrosis in the right ventricle were detected in all cases, myocarditis in 44%, mitral valve prolapse in 42%, and muscle fiber dyscomplexation in the right ventricular in 33%. The most informative signs for the diagnosis of arrhythmogenic right ventricular dysplasia using MRI are right ventricular contraction dyssynchrony and those using a morphological study are a residual cardiomyocyte area and muscle fiber atrophy in the right ventricle. A decrease in or focal absence of gamma-catenin expression in the muscle fibers was observed only in 60% of the patients.

Biolistic transformation of soybean by construction carrying a mutant alpha-tubulin gene as selective marker gene conferring resistance to dinitroaniline herbicides, and by additional construction pAHTUBI carrying a full-length barley beta-tubulin gene for correct co-expression of exogeneous tubulin in cells of transgenic soybean lines has been realized. It was established that 10 microM trifluralin is the most optimal selective concentration to pick up transformed soybean lines. Transgenic nature of offained regenerants was confirmed by Southern blotting hybridization using specific probe to selective alpha-tubulin gene.

Embryogenic potential of 19 soybean cultivars originated from different eco-geographic regions (Europe, China, America and Far East) has been established and analyzed. Cultivars TSZ-14, Amurskaja-111, Gribovskaja mestnaja were emphasized as highly embryogenic genotypes. Cultivar Mantherova biela vel. revealed the highest embryogenic potential (80%). The comparison of embryogenic potential of the Ukrainian cultivars with given data indicated various parameters which could affect the induction of soybean somatic embryogenesis. It had also been supposed that cultivars from the Far East and Europe could have the potential for further screening of new highly embryogenic genotypes. The data obtained enlarge the data base pool of the soybean somatic embryogenesis research and enable using the most appropriate cultivars for further genetic improvement.

Seven soybean (Glycine max (L.) Merr.) cultivars zoned in forest-steppe and marshy woodlands of Ukraine: "Chernuatka", "Vasylkivska", "Kyivska-91", "Kyivska-27", "Marjana", "Chernobura" and "Altair" were established in vitro for somatic embryogenesis inducing. Cultivars "Marjana" (88%) and "Vasylkivska" (86%) demonstrated the highest embryogenic capacity among all the tested cultivars. During the further plant regeneration cultivars "Marjana" and "Kyivska-91" showed the best capacity to form adult plants despite the fact that embryogenic capability of the cultivar "Kyivska-91" was 71%. According to the obtained data three genotypes were selected for the further investigation of effective methods of biolictic transformation of ukrainian cultivars.