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Multiple Sclerosis (MS) is a serve autoimmune neurodegenerative disease. Development of innovative approaches of MS treatment is of a high priority in the modern immunology and pharmacy. In the present study we showed high therapeutic efficiency of immunodominant peptides of myelin basic protein (MBP) incorporated into the monolayer mannosylated liposomes on the development of experimental autoimmune encephalomyelitis (EAE) in DA rats. MBP is a component ofoligodendrocytes' membrane, which form axonal sheath, and is one of the major autoantigens in MS. We analyzed binding pattern ofanti-MBP autoantibodies from MS patients using previously designed MBP epitope library. Utilizing the same approach we investigated pool of anti-MBP antibodies from SJL/J and C57/BL6 mice and DA rats with induced EAE. The most relevant rodent model to MS was EAE in DA rats according to the autoantibodies' binding pattern. We selected three immunodominant MBP fragments encapsulated in monolayer mannosylated liposomes for the following treatment of verified DA rodent model. MBP fragment 46-62 was the most effective in reducing of the first EAE attack, whereas MBP 124-139 and 147-160 inhibited development of pathology during remission stage. Simultaneous administration of these peptides in liposomes significantly decreased level of anti-MBP antibodies. Synergetic therapeutic effect of MBP fragments reduced integral disease score by inhibiting first EAE wave and subsequent remission, thus, our findings disclosure novel approaches for efficient treatment of Multiple Sclerosis.

Data on the composition of major and minor molecular forms of triacylglycerols from edible vegetable oils are reviewed. To estimate the food and biological value of vegetable oils, an attempt was made to classify them according to their triacylglycerol composition.

Three criteria have been suggested for the identification of food vegetable oils: the ratio of the summary area of triglyceride peaks and the summary area of the reference peaks; a set of peaks of critical pairs of oil triglycerides and their relationship expressed as the percent of the maximum peak. To prove the correctness of the identification a previously known oil is analyzed and its characteristics are compared to those of the oil under study. A mixture of saturated triglycerides with equivalent carbon numbers 30-56 is used as a reference one. A total of 16 vegetable oils have been identified with the use of the method suggested.

A spectrofluorometric method of analyzing the carcinogenic N-nitrosoamines in the form of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole derivatives is proposed. The content of nitroso-amines in a number of products of the vegetative origin was determined. In some samples of beetroots, radish and apples dimethyl-nitroso-amine in concentrations of 0.7--1.5 gamma/kg was detected. A conclusion is drawn on a relatively low content of carcinogenic N-nitroso-amines in the products of the vegetable origin.

The content of carcinogenous N-nitrosoamines (N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosopiperidine) was determined fluorimetrically (as NBD-amines) in preserved products, food concentrates, dried fruit and mushrooms. No N-nitrosoamines were found in the concentrates and dried products. The content of N-nitrosamines did not exceed 3 g/kg. In some samples its content reached 19 microgram/kg.

Samples of meat and meat products for the content therein of carcinogenic N-nitrose-amines were subjected to a fluorometric analysis. In case of positive results the presence of NA was confirmed by cromate-mass-spectrometric and/or mass-spectrometric methods. The mean value for the concentrations of dimethyl-nitrose-amine, diethyl-nitrose-amine and nitrose-piperidine in meat products is within the ranges of 1.5-5.4, 1.0-6.1, 0.9-23.4 gamma/kg, respectively. NA was not found in meat.