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Aim: To obtain the data on the spatial relationships between catecholamine (TH-positive) and nitroxidergic (nNOS-positive) neurons in vasomotor nuclei of the medulla in different periods of hypertension development., Material and Methods: The experiment was performed on male Wistar rats (n=45) with induced renovascular hypertension (RVH). TH and nNOS in neurons of solitary tract nuclei, reticular small-and giant cell nuclei were detected using immunohistochemical methods., Results and Conclusion: The most early and severe changes in the intensity of reaction and amount of nNOS-positive neurons were noted in the solitary tract nucleus. Significant changes in the quantitative parameters of TH-positive neurons in RVH were identified only in the reticular giant cell nucleus but they appeared later and were less expressed compared to nNOS-positive cells. This resulted in the changes of spatial relationships between two types of neurons and remodeling of the bulbar region of the cardiovascular center.

It was shown that GSH concentration and glutathione peroxidase activity in hypothalamus (H) and sensomotor cortex (SC) were twice more than in ME. After single or double injection of diethylmaleate (DEM) two-phase change of the GSH level was observed in all the investigated structures. In the first 1-3 h phase, the pool of free GSH decreases by more than 50% as a result of conjugative effect of DEM. In the second phase which is about 3 days long the level of GSH is gradually restored mainly due to the activation of its biosynthesis. In various brain structures conjugation and reduction processes has their own peculiarities: conjugation processes predominate in H and SC while the reduction processes predominate in ME. These processes were almost two times intensified in all the investigated structures under double injection of DEM.