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Objective: to analyze the 3D micro- and nanostructure and quantitative morphological parameters of rat lung tissue. Materials and methods. Wistar rat lung tissue samples were obtained for the study. The 3D structure of the lung tissue was studied via scanning probe nanotomography using an experimental setup combining an ultramicrotome and a scanning probe microscope. Results. Nanoscale images and 3D nanotomographic reconstructions of the interalveolar septal sections of the rat lung were obtained. Morphological parameters (average roughness and specific effective area) of the interalveolar septal surface were determined. It was found that the average roughness of the reconstructed septal surface was 345.4 ± 24.5 nm, and the specific effective area was 2.7 ± 0.2 units. Conclusions. Results obtained demonstrate that scanning probe nanotomography allows to quantify lung morphology. The use of scanning probe nanotomography for 3D analysis of the structure and characteristics of lung tissue will increase the efficiency of future developments on creation of new criteria for diagnosing pathological conditions.

Background. Despite the large number of studies devoted to the study of the functional state of the cardiovascular and respiratory systems after operations on the lungs, there are still many issues regarding the mechanisms of hemodynamic disorders in the postoperative period. It stipulates considerable difficulties in the treatment and prevention of functional disorders of respiration and circulation. Objective. The aim of this study is to determine the central hemodynamics parameters depending on the condition of ventilate lung function in thoracic oncology patients during the postoperative period and to determine the morphological features of the lungs that developed under the influence of intraoperative administration of refortan and perftoran. Methods. The condition of the central heamodynamic and morphological features of the lungs at patients with lung cancers with different level of the ventilating respiratory insufficiency in the early postoperative period after application of 3 and 1,5 ml/kg of perftoran and after traditional refortan (5 ml/kg) infusion-transfusion therapy were determined. Results and conclusion. It was proved that perftoran is much more effective than pefortan. The restoration of the normodynamic type of heamodynamic after infusion of perftoran (1,5 ml/kg) to patients with the an initial level of the ventilating respiratory insufficiency of I and II degree is observed during 1st postoperative week. The restoration of the normodynamic type of heamodynamic at to patients with the initial level of the ventilating respiratory insufficiency of III degree in postoperative period is reached by using perftoran in a dose 3 ml/kg. In the dead after pneumonectomy with intraoperative administration of perftoran there is increasing of synthetic activity of alveolocyte type II along with compensatory microcirculatory changes. Citation: Tverdokhlib IV, Novikov SP. [Сentral heamodynamic сondition and morphological characteristics of lungs at patients with lung cancer depending on components of the infusion-transfusion therapy]. Morphologia. 2015;9(4):76-84. Ukrainian.

Органы дыхания напрямую взаимодействуют с атмосферным воздухом и это ведет к проникновению таких вредных субстанций, как газ, туман, дым, пыль и аэрозоли в легкие. Пыль является наиболее важным фактором загрязнения воздуха на современном индустриальном предприятии и развитии последующих дисфункций легких. Морфологический метод исследования бронхиально-легочной системы является критерием оценки патогенных свойств вышеперечисленных вредных веществ. Этот метод применяется при гигиеническом нормировании промышленных аэрозолей. В последние годы отмечается рост профессиональных заболеваний легких, обусловленных пылью. Пыль хризотила в сочетании с действием других факторов приобретает более патогенные свойства, чем при изолированном действии. В настоящее время проблема использования хризотила – весьма острая мировая проблема., The respiratory organs directly interact with atmospheric air and this fact leads to the penetration of such harmful substances as gaseous fluid, fog, smoke, dust and aerosols into the lungs. And dust is the most harmful factor of the air pollution in the contemporary industrial production and the development of subsequent of the pulmonary dysfunction. The morphological method of studying of the bronchial - pulmonary system is one of the basic criteria of evaluation of pathogenic properties of above-mentioned harmful substances. The method out is applied during the hygienic rate-setting of the industrial aerosols. In recent years are marked by increase in occupational pulmonary diseases related to dust. Сhrysotile dust associated with other factors induces more hazardous effects, than if acting solely. At present, the use of chrysotile is a very acute global problem., Тыныс алу мүшелері атмосфералық ауамен тығыз қатынаста болуы әсерінен өкпеге әр түрлі(газ, тұман, түтін, шаң және басқа да аэрозольдар)зиянды заттардың түсуіне ықпал етеді. Қазіргі таңда өндірісте осы факторлардың ішінде ең негізгісі шаң болып табылады және өкпе дисфункциясына әкеледі. Бронх - өкпе жүйесін морфологиялық зерттеу әдісі зиянды заттардыңпатогенді қасиетін бағалаудың критериі болып саналады. Бұл әдіс өндірістік шаңдарды гигиеналық нормалауда қолданылады. Соңғы жылдарда шаң әсерінен пайда болатын кәсіби аурулардың санының өсуі байқалуда. Хризотил шаңының жекелей әсерімен салыстырғанда, басқа факторлармен бірігіп әсер еткенінде оның патогенді қасиеті біршама жоғарылай түседі. Қазіргітаңда хризотилді пайдалану – аса өткір әлемдік мәселе., Наука и здравоохранение, Выпуск 2 (17) 2015, Pages 30-38