1. Remote Ischemic Preconditioning Contributes to the Expression of Long Non-Coding RNA H19 and Hypoxia-Inducible Factor-1α during Isolated Coronary Artery Bypass Graft Surgery in Patients with Ischemic Heart Disease
- Author
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M. Khetsuriani, N. Ioffe, M. Rudenko, T. Drevytska, and V. Dosenko
- Subjects
long non-coding rna ,ischemic preconditioning ,h19 ,hif-1α ,ischemic heart disease ,coronary artery bypass grafting ,Surgery ,RD1-811 - Abstract
The aim. The aim of our study was to establish the expression levels of long non-coding RNA H19 and hypoxiainducible factor-1α (HIF-1α) in the myocardium and leukocyte fraction as a possible mechanism of adaptation to remote ischemic preconditioning (RIPC) in patients with ischemic heart disease during off-pump isolated coronary artery bypass grafting. Methods. To assess hemodynamic parameters, data from 31 patients (14 in the RIPC group, 17 in control group) were analyzed. The RIPC procedure was performed in patients before surgery by applying a blood pressure cuff to the right forearm. The cuff was inflated to a pressure of 200 mmHg and left for 5 minutes. This was followed by a reperfusion step which lasted 5 minutes. Periods of ischemia and reperfusion lasting 5 minutes were repeated three times. The expression level of long non-coding RNA H19 and HIF-1α was determined in the myocardium and leukocyte fraction by real-time polymerase chain reaction. Results. At the stage of formation of distal anastomoses in patients with RIPC cardiac index (CI) was 24% higher, and stroke volume index (SVI) was 18% higher. Systemic vascular resistance index (SVRI) was significantly lower in patients of the RIPC group (p 0.05). Conclusions. Based on hemodynamic parameters, it can be concluded that patients with RIPC were more hemodynamically stable. Significant changes in the expression of long non-coding RNA H19 and hypoxia-inducible factor-1α demonstrate the importance of these molecules in adaptation to ischemic preconditioning. However, the mechanisms of RIPC involving H19 and HIF-1α need further study.
- Published
- 2020
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