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In this study, we studied the use of physiological saline solution (NaCl 0,9%) or dulbecco’s phosphatebuffered saline (D-PBS) for mature sperms short-term preservation. After being collected from epididymides, sperms were adjusted to desired concentration (2x106 sperms/ml) with NaCl 0.9% solution or D-PBS solution (the dishes containing sperms were covered by mineral oil) and stored at 4oC, 10oC and room temperature (RT/26oC). The survival rate of sperms were evaluated by morphological observation and staining methods (single staining and double staining) every 8 hours. The results showed that the mouse sperms can be stored in a short-term at 4oC or 10oC without cryoprotectant agents, the survival rate in D-PBS solution are higher than those in NaCl 0,9% solution. The lethal concentration and time (LCt50) to mouse sperm in short-term preservation is 10 hours in D-PBS or NaCl 0,9% solution without cryoprotectant agents.