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Your search keyword '"Gram-Negative Chemolithotrophic Bacteria genetics"' showing total 2 results
Start Over Descriptor "Gram-Negative Chemolithotrophic Bacteria genetics" Language russian
2 results on '"Gram-Negative Chemolithotrophic Bacteria genetics"'

1. [Strain polymorphism of the plasmid profiles in Acidithiobacillus ferrooxidans].

Author

Kondrat'eva TF, Ageeva SN, Muntian LN, Pivovarova TA, and Karavaĭko GI

Subjects
Drug Resistance, Microbial, Environmental Microbiology, Gram-Negative Chemolithotrophic Bacteria drug effects, Gram-Negative Chemolithotrophic Bacteria metabolism, Metals, Heavy pharmacology, Oxidation-Reduction, Plasmids chemistry, Sulfites, Gram-Negative Chemolithotrophic Bacteria genetics, Plasmids analysis
Abstract

Plasmid profiles were studied in 27 Acidithiobacillus ferrooxidans strains isolated from different geographic zones and substrates differing in the composition of the main sulfide minerals, and also in experimentally obtained strains with acquired enhanced resistance to the ions of heavy metals (Fe, Ni, Cu, Zn, As). In 16 out of 20 strains isolated from different substrates, one to four 2- to 20-kb and larger plasmids were revealed. Plasmids were found in all five strains isolated from gold-containing pyrite-arsenopyrite ores and concentrates, in nine of 11 strains isolated from the ores and concentrates containing nonferrous metals, and in two of four strains isolated from the oxidation substrates of simple composition (mine waters, pyritized coals, active sludge). Changes in the plasmid profiles in some A. ferrooxidans strains (TFZ, TFI-Fe, TFV-1-Cu) with experimentally enhanced resistance to Zn2+, Fe3+, and Cu2+, respectively, were noted as compared with the initial strains. After 30 passages on S0-containing medium, strain TFBk showed changes in the copy number of plasmids. The role of plasmids in the processes of oxidation of energy substrates and in the acquired enhanced resistance to the heavy metal ions is discussed.

Published
2002

2. [Determination of the phylogenetic position of Sulfobacillus thermosulfidooxidans on the basis of analysis of the 5S and 16S ribosomal RNA].

Author

Turova TP, Poltoraus AB, Lebedeva IA, Bulygina ES, Tsaplina IA, Bogdanova TI, and Karavaĭko GI

Subjects
Base Sequence, Clostridium genetics, Clostridium metabolism, Energy Metabolism physiology, Gram-Negative Chemolithotrophic Bacteria metabolism, Iron metabolism, Molecular Sequence Data, Gram-Negative Chemolithotrophic Bacteria genetics, Phylogeny, RNA, Ribosomal, 16S analysis, RNA, Ribosomal, 5S analysis, Sulfur metabolism
Abstract

The nucleotide sequence of the 16S rRNA gene of the type strain Sulfobacillus thermosulfidooxidans VKM-1269 was determined. This microorganism belongs to the group of thermophilic acidophilic chemolithotrophic bacteria capable of utilizing elemental sulfur and iron ions as the sole energy sources. The phylogenetic position of the bacterium studied among Gram-positive bacteria was estimated by a comparative analysis of 16S rRNA nucleotide sequences. It was shown that Sulfobacillus forms a coherent group with species of a new genus Alicyclobacillus. The Sulfobacillus-Alicyclobacillus group is suggested to represent a separate evolutionary branch within the clostridial subdivision of Gram-positive bacteria.

Published
1995

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