1. [Comparison of protective properties of the smallpox DNA-vaccine based on the variola virus A30L gene and its variant with modified codon usage].
- Author
-
Maksiutov RA and Shchelkunov SN
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Viral immunology, Base Sequence, Chlorocebus aethiops, Codon genetics, Female, Gene Expression genetics, Genes, Synthetic genetics, Genes, Synthetic immunology, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Smallpox Vaccine administration & dosage, Smallpox Vaccine genetics, Vaccination, Vaccines, DNA administration & dosage, Vaccines, DNA genetics, Variola virus genetics, Vero Cells, Viral Proteins genetics, Smallpox prevention & control, Smallpox Vaccine immunology, Vaccines, DNA immunology, Variola virus immunology, Viral Proteins immunology
- Abstract
Efficacy of candidate DNA-vaccines based on the variola virus natural gene A30L and artificial gene A30Lopt with modified codon usage, optimized for expression in mammalian cells, was tested. The groups of mice were intracutaneously immunized three times with three-week intervals with candidate DNA-vaccines: pcDNA_A30L or pcDNA_A30Lopt, and in three weeks after the last immunization all mice in the groups were intraperitoneally infected by the ectromelia virus K1 strain in 10 LD50 dose for the estimation of protection. It was shown that the DNA-vaccines based on natural gene A30L and codon-optimized gene A30Lopt elicited virus, thereby neutralizing the antibody response and protected mice from lethal intraperitoneal challenge with the ectromelia virus with lack of statistically significant difference.
- Published
- 2011