1. [Method of studying the functional state of pulmonary alveolar macrophages during exposure to atmospheric pollutants].
- Author
-
Koffin D, Gardner D, Voters M, Merkur'eva RV, and Litvinov NN
- Subjects
- Acid Phosphatase metabolism, Animals, Cell Count, Galactosidases metabolism, Glucosidases metabolism, Glucuronidase metabolism, Hexosaminidases metabolism, Macrophages immunology, Muramidase metabolism, Phagocytosis, Rabbits, Rats, Air Pollutants toxicity, Macrophages enzymology, Pulmonary Alveoli cytology
- Abstract
On the basis of experimental research results a method to assess the functional state of pulmonary alveolar macrophages in rabbits and rats has been proposed as a criterion of the biological effect of chemical atmospheric pollutants. The test involves a cytological assay, determination of the viable cells quantity and of the phagocytic competence, and also the biochemical study of alveolar macrophages enzymes activity (acid phosphatase, beta-glucuronidase, lysozyme, beta-galactosidase, beta-glucosidase, N-acetyl-beta-D-glucosaminidase). It has been shown that this method is informative and reliably reproducible, and that it was reasonable to use it in environmental health and other branches of experimental biology and medicine.
- Published
- 1978