1. [Influence of regulatory T cells on the functioning of natural killer cells during cancer immunotherapy].
- Author
-
Chikileva IO, Shubina IZh, and Kiselevskiĭ MV
- Subjects
- CD4-Positive T-Lymphocytes metabolism, Cells, Cultured, Cytotoxicity, Immunologic, Forkhead Transcription Factors metabolism, Humans, Immunotherapy, Interleukin-2 immunology, Interleukin-2 pharmacology, Interleukin-2 Receptor alpha Subunit metabolism, Killer Cells, Natural immunology, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear immunology, Neoplasms therapy, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology
- Abstract
One of the common arguments against cancer immunotherapy based on natural killer (NK) cells activated in the presence of interleukin-2 (IL-2) is the probability of the activation of regulatory T cells (Tregs) by IL-2 besides NK cells. Thus, we have monitored numbers of FoxP3+CD4+CD25+ T cells in the samples of healthy volunteers' peripheral blood mononuclear cells (PBMCs) cultured with or without IL-2. We observed marked increase in the percentages of the CD4+CD25+ T cells in the presence of IL-2. Proportions of Foxp3+CD4+CD25+ T cells feebly increased, remained on the same level or even decreased compared to PBMCs cultured without exogenous IL-2. Based on the absence of FoxP3 expression, most of the CD4+CD25+ T cells purified from IL-2 activated PBMCs were not Tregs, but activated Th cells. Moreover, the addition of the purified supposed Tregs to samples of activated NK cells never inhibited their cytotoxic reactions.
- Published
- 2012