Analysis and identification of 90 specimens of genus Acentrogobius bleeker, 1874 collected from estuary and coastal of Nghe An province, Vietnam. We have classified five species: Acentrogobius caninus (Valenciennes, 1837); Acentrogobius chlorostigmatoides (Bleeker, 1849); Acentrogobius moloanus (Herre, 1927); Acentrogobius cf. viridipunctatus (Valenciennes, 1837) and Acentrogobius cf. viridipunctatus (Valenciennes, 1837).The species Acentrogobius moloanus (Herre, 1927) and Acentrogobius cf. viridipunctatus (Valenciennes, 1837) was first discovered in the study area and the north center region of Vietnam