1. [FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA].
- Author
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Ulshina DV, Kovalev DA, Zhirov AM, Zharinova NV, Khudoleev AA, Kogotkova OI, Efremenko VI, Evchenko NI, and Kulichenko AN
- Subjects
- Agar chemistry, Agar pharmacology, Bacterial Proteins biosynthesis, Brucella abortus growth & development, Brucella abortus metabolism, Brucella melitensis growth & development, Brucella melitensis metabolism, Culture Media chemistry, Metabolome drug effects, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Bacterial Proteins isolation & purification, Brucella abortus drug effects, Brucella melitensis drug effects, Culture Media pharmacology
- Abstract
Aim: Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar., Materials and Methods: Vaccine,strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex "Bruker Daltonics" MALDI-TOF mass-spectrometer., Results: A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used., Conclusion: Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
- Published
- 2016