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To investigate the complexation of L-tyrosine (Tyr) with hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-α-L- arabinopyranoside (α-hederin) and its 28-O-α-L-rhamnopyranosyl-(1 → 4)-O-β-D-glucopyranosyl-( 1→6)-O-β-D-glucopyranosyl ester (hederasaponin C); to study the effect of glycosides, Tyr, and their mixtures on Avena sativa L. seed germination. Methods. Electrospray ionization mass spectrometry. Results. Mass spectra of mixes of glycosides with Tyr have been received and interpreted. The comparative analysis of biological activity of individual glycosides and their complexes with Tyr and others aromatic amino acids has been performed. Conclusions. For the first time a composition of the complexes is established by mass spectrometry. The complex of β-hederin with Tyr has appeared most toxic. [ABSTRACT FROM AUTHOR]

Aim: Study the composition of plant extracts using high-performance liquid chromatography. (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor., Materials and Methods: Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method., Results: Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V. cholerae El Tor, were determined., Conclusion: The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in xtracts, possessing antimicrobial effect against V. cholera El Tor strains.

The molecular complexation of adenine and guanine with hederagenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-alpha-L-arabinopyranoside (alpha-hederin) and its 28-O-alpha-L-rhamnopyranosyl-(1-->4)-O-beta-D-glucopyranosyl-(1-->6)-O-beta-D-glucopyranosyl ester (hederasaponin C) was investigated for the first time using the method of electrospray ionization mass spectrometry. Guanine forms complexes more diverse in composition than adenine.

Our studies demonstrated that chronic opisthorchosis with a skin syndrome is accompanied by a severer clinical course. The course of rehabilitation measures with the use of resort recreation (mud-bad cure) combined with traditional treatment stages makes it possible to considerably enhance the efficiency of treatment of both opisthorchosis and its skin manifestations, and should be applied more often in practice.

Formation of a healthy life style is a complex socioeconomic task which is to be solved at the level of the state, public health care agencies as well as at the individual level. Attainment of this objective will require a complex approach to each individual taken separately.

The immune and fibrinolytic systems were studied in 55 patients with postpneumonic lung abscess; 17 of them were operated on. Revealed were reductions of the number and functional activity of the T-cell immunity and a systemic-regional fibroanalysis data (high fibrinolytic activity in the systemic circulation and bronchial lumen; high activity in the blood of the resected lung portion). High activator activity of the sputum and its absence in the pus of the abscess cavity evidence that the source of the plasminogen activator is the bronchial wall not the destructed pulmonary tissue.

Women suffering of infectious-dependent bronchial asthma show at the second half of pregnancy a sharp reduction of the fibrinolytic potential that is manifested in a decrease of plasminogen activator and urokinase activity of the urine, in an elevation of the antiactivator and antiplasmin activity, growth of the concentration of alpha2-macroglobulins. At the same time high levels of fibrinogen are noted and low levels of fibrin degradation.

The leading role of allergic components was established in some rare patterns of prostatitis. To develop the methods applicable for differential diagnosis of allergic prostatitis, the authors followed up 60 patients aged from 15 to 53 years who suffered from chronic prostatitis and 10 normal controls. The patients with bacterial disease, abacterial prostatitis with concurrent allergic components or subjects with allergic prostatitis were enrolled in the study. Immunoassay was an essential part in the diagnosis of allergic prostatitis. The study revealed a dramatic inhibition of ejaculate gamma-glutamine transferase in those who suffered from an abacterial pattern with allergic component and almost three-fold in those with allergic prostatitis. Certain though insignificant decrease in the ejaculate gamma-glutamine transferase was documented in patients with bacterial prostatitis. Investigations of ejaculate IgE revealed quite an opposite picture: 10-fold increase in IgE levels in the ejaculate of patients with allergic prostatitis as compared with other groups, controls in their number. Besides, patients with allergic prostatitis had three-fold higher IgE in the ejaculate than in the blood serum. The aforementioned phenomenon failed to be observed in patients with other patterns of prostatitis. Decreased ejaculate gamma-glutamine transferase and increased IgE can be regarded as criteria for differential diagnosis of allergic prostatitis.

Design improvement of machines and devices for seed decontamination with account of the proposed hygienic recommendations promoted sanitation of operators' working conditions. Introduction of progressive technology (damping, inlaid work) enables one to reduce pesticide application and to decrease ecologic hazard of seed chemical protection.

Pesticide spraying machines for agricultural crops were hygienically assessed, the analysis covered the machines of three generations. The prospects of quick introduction of scientific hygienic achievements into the production of machines intended for chemization of agriculture lie in creative cooperation between engineers and hygienists.