1. [DNA methylation profiling of the vascular tissues in the setting of atherosclerosis].
- Author
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Nazarenko MS, Markov AV, Lebedev IN, Sleptsov AA, Frolov AV, Barbash OL, and Puzyrev VP
- Subjects
- Atherosclerosis genetics, Atherosclerosis pathology, Female, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Humans, Male, Mammary Arteries pathology, Middle Aged, Proteins genetics, Proteins metabolism, Saphenous Vein pathology, Atherosclerosis metabolism, CpG Islands, DNA Methylation, Mammary Arteries metabolism, Saphenous Vein metabolism
- Abstract
To date the question of epigenetic mechanisms of gene regulation in the context of cardiovascular diseases is of a considerable interest. Here, for the first time DNA methylation profiles of vascular tissues of atherosclerotic patients have been analyzed with using the microarray Infinium HumanMethylation27 BeadChip ("Illumina", USA). As the result, within 286 genes 314 CpG-sites that varied significantly in the DNA methylation level between the tissue samples of carotid (in the area of atherosclerotic plaques and nearby macroscopically intact tissues of the vascular wall) and mammary arteries as well saphenous veins have been identified. The most pronounced differences in the methylation level were registered for CpG-sites of homeobox genes HOXA2 and HOXD4 as well as imprinted gene MEST. In particular, these genes were found to be hypomethylated in the carotid atherosclerotic plaques compared to their methylation patterns in intact tissues of internal mammary arteries and saphenous veins.
- Published
- 2013
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