1. [Treatment with anti-cancer agents results in profound changes in lncRNA expression in colon cancer cells].
- Author
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Zinovieva OL, Grineva EN, Prokofjeva MM, Karpov DS, Krasnov GS, Prassolov VS, Mashkova TD, and Lisitsyn NA
- Subjects
- Cell Line, Tumor, Colonic Neoplasms drug therapy, Colonic Neoplasms genetics, Colonic Neoplasms pathology, Humans, RNA, Long Noncoding genetics, RNA, Neoplasm genetics, Antineoplastic Agents pharmacology, Colonic Neoplasms metabolism, Gene Expression Regulation, Neoplastic drug effects, RNA, Long Noncoding biosynthesis, RNA, Neoplasm biosynthesis
- Abstract
Using real-time RT-PCR in combination with bioinformatics, we have shown for the first time that the treatment of HCT-116 and HT-29 colon cancer cells with two anti-cancer agents (doxycycline or 3,3'-diindolylmethane) results in profound changes in the intracellular content of several lncRNAs (by up to 100 times). Since many of these RNAs are secreted by tumors into the bloodstream, the obtained results provide a basis for developing more sensitive protocols for serological monitoring of tumor relapse and metastasis, as well as for search of new anti-cancer drugs.
- Published
- 2017
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