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Introduction. The rapid spread of coronavirus infection COVID-19 among the population of many countries around the world has contributed to the emergence of many genetic variants of SARS-CoV-2. Compared to previous coronavirus variants, the new Omicron variants have shown a noticeable degree of mutation. Virus inactivation is one of the most important steps in the development of inactivated vaccines. The chemical inactivation agents currently used are β-propiolactone and formaldehyde, but there is no uniform standard for designing and specifying the inactivation process. Objective. Evaluation and comparison of the effectiveness of chemical inactivation of two agents, formaldehyde and β-propiolactone against immunogenicity of the Omicron variant of the SARS-CoV-2 virus. Materials and methods. Nasopharyngeal swabs were used to obtain the SARS-CoV-2 Omicron variant virus. Vero cell culture was used to isolate, reproduce, titrate the virus, and perform a neutralization reaction. The kinetics of studying the inactivation of the virus by chemical agents such as formaldehyde and β-propiolactone was carried out. Results. Studies have been conducted to comparatively evaluate the effectiveness of chemical agents used to inactivate the SARS-CoV-2 virus of the Omicron variant, planned for use in the production of an inactivated whole-virion vaccine. Formaldehyde and β-propiolactone were used as inactivation agents in concentrations of 0.05, 0.1, 0.5% of the total volume of the virus suspension. It has been established that complete inactivation of the virus by formaldehyde in the concentrations used at a temperature of 37 °C occurs within up to 2 hours, and when using beta-propiolactone, within up to 12 hours. Conclusion. Inactivated virus samples have different antigenic activity depending on the concentration of the inactivation agents used. The most pronounced antigenic activity is manifested in samples of the pathogen that were treated with an inactivation agent at a mild concentration of 0.05%. Increasing the concentration of inactivation agent by 5 or more times leads to a significant decrease in the antigenicity of the SARS-CoV-2 virus. With the inactivation modes used, the loss of biological activity of the virus occurs faster and antigenicity is retained largely when treated with formaldehyde.

Introduction. The lack of data on the whole-genome sequences of African swine fever virus (ASFV) variants circulating on the territory of the left bank of the Dnieper River complicates the understanding of the molecular evolution of the virus and the character of the epidemic process development in Russia and Ukraine. Understanding the genetic divergence and phylogenetic relatedness of isolates can largely adjust the strategy of general and specific prevention of the disease. The aim of the study – search and description of unique mutations (deletions/insertions/substitutions) in isolates collected from domestic pigs in Donetsk, Luhansk and Zaporozhye regions in 2023; determination of relatedness and level of homology with reference strains of ASFV genotype II; sub-genotyping and clustering of isolates based on whole-genome analysis. Materials and methods. The samples used were a culture suspension of porcine bone marrow (PBM) cells containing ASFV isolates obtained from pathologic material from domestic pig carcasses. Genomic DNA was prepared by purification and concentration of virus followed by phenol-chloroform extraction of total nucleic acid. The high-throughput sequencing process was performed using MGI technology. Consensus sequences were assembled by mapping reads to the reference genome of strain Georgia 2007/1. Results. All isolates are assigned to genotype II, have a monophyletic origin, are phylogenetically close to the clusters «Europe» (4/5) and «Bryansk 2021» (1/5), and are divergent from the original parental genetic variants that make up the enlarged clades. In addition, numerous substitutions in the loci of the multigene family MGF 110, 505, and 360, encoding virulence proteins, were detected in 4 isolates from Donetsk and Zaporozhye regions. Conclusion. The phylogeny of the genotype II ASFV, which originated from the reference strain Georgia 2007/1, is shown to be sufficient for isolate differentiation. The presented data are of theoretical and practical importance for domestic and international ASFV surveillance.

INTRODUCTION. The degree of processing (manipulation) of cells included in a cell product and the functions performed after administration (homologous/non-homologous use) determine the classification of the cell product as a transplant or an advanced therapy medicinal product (ATMP) and, hence, the regulatory aspects of the product’s life cycle. Currently, the legislation of the Eurasian Economic Union (EAEU) and the Russian Federation does not sufficiently explain the terms ‘minimal manipulation’ and ‘homologous/non-homologous use’, which may lead to the use of cell products with unproven safety and efficacy in humans.AIM. This study aimed to compare Russian and international approaches to the interpretation of the terms ‘minimal manipulation’ and ‘homologous/non-homologous use’ for classifying cell products and determining their regulatory pathways, with stromal vascular fraction (SVF) products used as an example.DISCUSSION. This article reviews and summarises the regulatory approaches of the Russian Federation, the EAEU, the United States (US), and the European Union (EU) that are based on the classification of cell products according to the degree of cell manipulation and the functions performed after administration. The authors have analysed and compared the regulatory acts and approaches of the countries under consideration, with SVF products as a case study. The article highlights general aspects of interpreting the terms ‘minimal manipulation’ and ‘homologous/ non-homologous use’ and demonstrates the difference in regulatory approaches across several countries, which lies in the classification of enzymatic processing and selective collection of cells as substantial or minimal manipulation.CONCLUSIONS. The mechanism for regulating cell products depends on the degree of cell manipulation (substantial or minimal) and the intended use (homologous or non-homologous). A common principle adopted by regulatory agencies in the US, EU, EAEU, and Russia is to classify manipulation as minimal if the manipulated cells preserve their biological characteristics and physiological function. A defining characteristic of the homologous use of cells or tissues is their administration to perform their inherent functions in the body. In Russia, the regulatory acts for ATMPs and for transplants list the procedures classified as minimal manipulation. According to international standards, preparations based on minimally manipulated SVF cells are classified as ATMPs when used non-homologously. The lack of comprehensive and clear explanations of the terms ‘minimal manipulation’ and ‘homologous/non-homologous use’ in the legislation of the EAEU and the Russian Federation necessitates the development of relevant guidelines providing specific examples.

Recent investigations have demonstrated the involvement of neutrophils and neutrophil extracellular traps (NETs) in the initiation and progression of autoimmune inflammation in rheumatoid arthritis (RA) patients. Simultaneously, macrophages/monocytes, which are the main antigen-presenting cells, play a key role in the immune response as a result of disruption of antigen processing and presentation. Studying the role of monocytes and monocyte extracellular traps (METs) in RA pathogenesis is promising and is of particular interest. The aim of the research was to study the features of the main parameters of monocyte extracellular generation traps in RA patients depending on the stage of autoimmune inflammation. Material and methods. 37 RA patients and 30 healthy individuals were enrolled in study. Circulating monocytes were purified with centrifugation procedure using iohexol with a 1068 kg/m3 density gradient. Cells were identified histochemically, and the extent of monocyte activation was assessed using common nitroblue tetrazolium test. The generation of METs was stimulated by phorbol-12-myristate-13-acetate (PMA), and visualized using fluorescence microscopy with SYBR green. Results and discussion. In patients with inactive RA, the number of monocytes in a state of generating both spontaneous and PMA-induced METs is higher compared to the reference group. The transformation of autoimmune inflammation from remission to the active phase was accompanied by a further increase in the proportion of monocytes that form METs spontaneously and in the presence of PMA (by 114.7 % and 44.2 %, respectively, relative to the comparison group). The growth rate of spontaneous METs formation is 2.6 times higher than induced METs; RA activation is evidenced by an increase in the number of monocytes in the process of spontaneous formation of METs by more than 17.8 %. Conclusions. The obtained data suggest METs participation in the initiation and maintenance of RA autoimmune inflammation along with NETs presumably through exhibition their citrulline neoepitopes. METs could probably be considered as a new potential diagnostic biomarker.

A mucocele of the appendix is a cystic formation of the appendix, the lumen of which is filled with mucin of varying consistency. There are benign and malignant forms of the disease. Its pathogenesis varies depending on the etiology and has not yet been fully studied. The clinical picture of this disease is nonspecific and there are difficulties in preoperative diagnosis. The purpose of the study is to present a rare clinical case of mucocele of the appendix, as well as literature data on this pathology. Successful treatment of a patient with appendix mucocele, an atypical location of the tumor was demonstrated. Based on the results of intraoperative express biopsy, as well as postoperative pathomorphological examination, surgical intervention was carried out to the required extent and with a favorable prognosis for the patient’s future life. Choosing the right method of surgical treatment allows you to avoid dangerous complications, such as the development of mucinous cystadenocarcinoma and pseudomyxoma peritonei. When performing surgery, it is necessary to be guided by the principles of safety from the patient’s point of view and not be afraid of performing access conversion.

The liver size is important to take into account when it is necessary to assess the course and severity of various diseases, including infectious, malignant and systemic diseases. The liver linear dimensions of the same patient depend on the method of examination (palpation, percussion, sonography, computed tomography, magnetic resonance imaging). The objective of this investigation was to compare the linear diameters of the liver according to ultrasound data of cadavers before autopsy and liver linear diameters after extraction of it from the abdominal cavity. Material and methods. All data were obtained in the pathology department of the Regional Clinical Hospital of the Kaliningrad Region. We examined 25 cadavers (12 females and 13 males). Before the autopsy we performed the ultrasound examination with measuring of the linear diameters of the functional left and right lobes with a convex transducer of a portable S6 ultrasound system (SonoScape, China). After the autopsy we did 3 sagitally oriented incisions for maximum linear measurements similar to previous ultrasound ones. Results and discussion. After extraction of the liver from the abdominal cavity the anteroposterior diameters of both lobes decreased on average by half after autopsy (p < 0.001), the craniocaudal diameters of the left and right lobes, on the contrary, increased on average 2 times and 1.25–1.35 times respectively (p < 0.001). The oblique craniocaudal maximum diameter of the right lobe increased (“stretches”) by an average of 1.11–1.15 times (p = 0.002). Conclusions. After extraction of the liver from the abdominal cavity all the linear diameters changed. The oblique craniocaudal maximum diameter of the right lobe is the most “stable”, due to which it possible to imagine the intravital diameter of the liver.

Natural killer cells (NK cells) are a population of innate immune lymphocytes capable of cytolysis of infected or transformed cells without prior sensitization. Natural killers are detected in various organs and tissues and may differ in phenotypic and functional characteristics depending on localization. For example, NK cells are the dominant population (up to 70%) of decidual lymphocytes in early pregnancy. NK cells are able to contact with trophoblast cells, exert cytotoxicity towards them, as well as regulate their invasion, contributing to spiral arteries remodeling and establishment of physiological blood flow between mother and fetus. The contribution of impaired NK cell functional activity to immune mechanisms of the reproductive disorders is widely discussed. Various drugs are used to treat infertility, including intravenous immunoglobulins (IVIG) and recombinant granulocyte colony stimulating factor (G-CSF). Increased rates of embryo implantation and higher frequency of successful gestation have been shown after treatment with these drugs. The effect of these drugs on NK cells phenotype and functional activity is assumed, thus requiring further studies on the effects of IVIG and G-CSF on the receptor profile of NK cells. The aim of this work was to evaluate expression of cytotoxic receptors on the NK-92 cells in presence of IVIG and recombinant G-CSF preparations. NK-92 cells were used as effectors, and trophoblast-derived JEG-3 line served as target population. The cells were co-cultured in presence of drugs, as well as without them. Expression of CD45, CD56, CD215, KIR2DL3, KIR2DS4, NKG2D, NKp44, NKp30 receptors by NK-92 cells was evaluated by flow cytometry. The number of NK-92 cells expressing NKG2D, NKp30, KIR2DL3 receptors and the expression intensity of NKG2D and NKp30 receptors were reduced in presence of IVIG preparations. The numbers of KIR2DL3+ and NKp44+ NK cells were reduced when supplied with G-CSF and trophoblast cells. The obtained results may be associated with both direct and indirect effects of the studied drugs on the NK cell phenotype.

Dysembryoplastic neuroepithelial tumor (DNET) is a benign glioneuronal neoplasm, usually found in children and adolescents, in the vast majority of cases associated with drug-resistant epilepsy. Typically, epileptic seizures are the main, and in most cases, their only clinical manifestation. Although DNET is a benign, biologically stable tumor with few reports of malignancy, it is one of the most common reasons for epileptic surgery. The epileptogenic potential of this tumor is so high that DNET s, along with ganglioglioma, have received the informal term “epileptomas” and are by far the leaders in the group of low-grade tumors associated with long-term epilepsy-associated tumors (LEAT). It is believed that this epileptogenicity is due to localization in the neocortex and frequent association with focal cortical dysplasias (FCD). In the world literature, there are only a few mentions of DNET s not associated with epilepsy. The article presents the experience of complex, interdisciplinary diagnosis of DNET in a child without epilepsy who complained of frequent headaches. During a comprehensive MRI examination, a cortical-subcortical pathological substrate was discovered in the left temporal lobe with radiological signs of DNET. During video-EEG monitoring of night sleep, no epileptiform signs were recorded. There was no history of epileptic seizures or other paroxysms. A control MRI revealed a slight increase in the size of the pathological substrate, which was the reason for surgical treatment. Pathological examination revealed microscopic features of DNET. This case of absence of epilepsy in a child with cortical DNET in the temporal lobe cortex suggests that the spectrum of its clinical manifestations and biological behavior is not fully understood and requires further comprehensive study.

Introduction. Mutations in a GBA1 gene, which encodes a lysosomal enzyme called glucocerebrosidase (GCase), are the most common genetic risk factor for Parkinson's disease (PD). The pathogenesis of PD results from the death of dopaminergic neurons in the substantia nigra of the brain, which is associated with the aggregation of α-synuclein protein. However, not all GBA1 mutation carriers develop PD during their lifetime. The aim of this study was to evaluate GCase activity and α-synuclein levels in CD45+ blood cells of patients with PD associated with GBA1 mutations (GBА1-PD), asymptomatic carriers of GBA1 mutations (GBА1-carriers), and patients with sporadic PD (sPD), as well as correlation between the study parameters in the study groups. Materials and methods. The study included patients with GBА1-PD (n = 25) and sPD (n = 147), and GBА1-carriers (n = 16). A control group included healthy volunteers (n = 154). The level of α-synuclein in CD45+ cells was measured by enzyme-linked immunosorbent assay, and GCase activity in dried blood spots was detected by high-performance liquid chromatography with tandem mass spectrometry. Results. Increased level of α-synuclein protein was detected in CD45+ blood cells of patients with GBA1-PD, sPD, and GBA1-carriers compared to controls (p = 0.0043; p = 0.0002; p = 0.032, respectively). Decreased GCase activity was reported in GBA1-PD patients and GBA1-carriers compared to sPD patients (p = 0.0003; p = 0.003, respectively) and controls (p 0.0001; p 0.0001, respectively). However, negative correlation between α-synuclein levels and GCase activity was observed only in GBA1-PD patients, but not in GBA1-carriers. Conclusion. Our data suggest a possible functional relationship between the activity of GCase and the metabolism of α-synuclein in PD associated with GBA1 mutations.

In the presented work, the issues of measuring the thrust force of spacecraft thrusters are considered. A program for determining the thrust force based on machine vision has been developed. A mathematical model of the mechanical part of the stand has been compiled. Numerical modeling of the compiled system of equations is carried out. The simulation results are processed using the developed program. The relative error of misalignment is no more than 5 %. Field experiments are carried out and the thrust levels of the ion thruster are measured. A comparative analysis of the experimental results obtained with a mathematical model is shown. Experimental studies have been conducted to determine the thrust of a prototype arcjet thruster using a stand based on machine vision and using a stand based on a displacement sensor. The discrepancy in the results of the thrust force is  5,6 %.

The article is devoted to the study, restoration, and reconstruction of a poplar box originating from kurgan No. 13 of the Urysay-2 burial site. This box contained a set of ritual objects associated with a female “priestess”. The box was positioned behind the head of the deceased and was a low, square container with straight sides and a separate flat lid. The specific set of objects found in the box included a mirror, a cedar block, a “spoon” with traces of paint, a small organic vessel (leather? felt?), and silk fabric, supplemented by a small stone altar and a set of tools found nearby. During fieldwork, the box was removed in one block. Subsequently, an MRI scan was performed; a comprehensive set of procedures was conducted to identify the material of the finds, their physicochemical composition, conservation, and restoration; a 3D reconstruction of the box and the items was carried out.

Influenza and acute respiratory viral infections (ARVI) impose a substantial damage to the population health in the Russian Federation due to their seasonal circulation and predominantly affect young children. A very few data on the cytokine profile, the nonspecific LPO — AOD system and their relationships with clinical characteristics in such diseases in preschool children are available. The aim of this study was to assess the cytokine profile, LPO — AOD system parameters and their relationship with the clinical and laboratory characteristics of diseases in preschool children with influenza and other ARVI. 86 preschool children (3–6 years old) were examined: with an established diagnosis of influenza (n = 31), non-influenza ARVI (n = 28), apparently healthy children (control group (n = 27). All pediatric samples were analyzed by enzyme-linked immunosorbent assay assessing blood serum concentrations of C-reactive protein and cytokines IL-1β, IL-4, IL-6, IL-8, TNFα, IFNα, IFNγ. Spectrophotometric, fluorometric and enzyme immunoassay methods to assess the state of the “LPO — AOD” system were used. In the group of children with influenza vs other ARVI, a higher incidence of intoxication syndrome was revealed. Cytokine profile in children from both clinical groups compared with control cohort was featured with higher indicators of both pro-inflammatory and anti-inflammatory origin. Children with non-influenza ARVI, had increased magnitude of LPO final products in the nonspecific LPO — AOD system along with lowered concentration of fat-soluble vitamins, general antioxidant activity, GSH level, and SOD activity. In the group with influenza, the level of primary and final lipid peroxidation products was increased, whereas that of for retinol,α-tocopherol, and total antioxidant activity was decreased paralleled with higher GSSG and SOD levels. Numerous correlations were noted in the group of children with ARVI: IL-1β/ketones, IL-6/ketones, IL-8/ketones, TNFα/ketones, IL-4/ketones, IFNg/shortness of breath, IFNα/cough, double bonds/fever, double bonds/AST, SO/intoxication, retinol/fever, GSSG/cough. The influenza group differed in the following relationships: IL-4/ketones, IL-4/fever, IFNα/ketones, CDs/AST. It can be concluded that in preschool children with ARVI and influenza, changes in the cytokine profile are accompanied by increased pro- and anti-inflammatory cytokine levels, increased intensity of lipid peroxidation reactions along with reduced magnitude of antioxidant factors. In the group with ARVI, there was a relationship between the final toxic products of lipid peroxidation — Schiff bases — and the intoxication index, as well as the presence of protective mechanisms in the form of connections between interferons and disease clinical manifestations. The group with influenza was distinguished by the presence of protective relations, which may have a beneficial effect in the context of developing pathological process. The data obtained will help expand the understanding of the pathogenetic mechanisms related to immune reactivity and nonspecific lipid peroxidation reactions in preschool patients and formulate appropriate measures for correction.

Human papillomavirus (HPV) represents one of the most serious global public health problems. Malignant female and male diseases mainly result from persistent HPV infection. Cancer belongs to a high mortality rate disease. It has been established that HPV infection causes about 70% vaginal cancer, 50% male genital cancer, 90% anal cancer and 60% head-and-neck cancer. Annually, a large number of people develop various HPV-caused cancer types, dominated by cervical cancer, one of the most common and aggressive types of cancer that threatens health holding the fourth place among most female common cancer worldwide. According to the World Health Organization (WHO), in 2020, about 600 cases of cervical cancer are recorded daily in different countries. Emergence of cervical cancer is closely related to factors such as long-term (persistent) HPV infection and somatic mutations of the host genome. Although HPV infection can be detected and cured early with highly effective screening methods and surgical procedures, the carcinogenic risk of HPV related diseases constantly increases, which elimination faces certain difficulties, especially in low- and mid-developed countries. The most acceptable solution to this is development and implementation of therapeutic vaccines for prevention and treatment of HPV related diseases. Three licensed HPV vaccines based on L1 type virus-like particles (L1-VLPs) technology are available globally: bivalent (HPV-2), quadrivalent (HPV-4) and nonavalent (HPV-9) vaccines. These vaccines demonstrated effectiveness in reducing HPV-related cervical cancer rate by up to 90% worldwide. However, the therapeutic effect of these vaccines on persistent HPV infection and lesions has not been observed. Therapeutic HPV vaccines candidates targeted Ye6/Ye7 cancer proteins activate cellular immunity that eliminates existing HPV infection. Here we review types, mechanisms of action and clinical effects of therapeutic HPV vaccines, as well as current and future developments in the field for prevention and treatment of HPV related diseases.

Background. Increasing the production of native and modified starch from maize requires raw materials with high starch content in grain. Materials and methods. An experimental panel of 780 simple high-starch maize hybrids produced with CMS-S and CMS-C lines underwent two-year testing. Starch content in the grain of the lines and their hybrids was assessed with IR spectrometry. Native starch content in the grain of hybrids with highest yields was measured at the All-Russian Research Institute of Starch and Starch-Containing Raw Materials Processing using the method proposed by L. P. Nosovskaya with coauthors. Responses to CMS were scored according to G. S. Galeev’s scale. Results. Grain starch content was found to vary from 58% to 72% DMB throughout the tested panel. IR spectrometry helped to identify 22 hybrids with high (72.03–72.67%) starch content, and 5 hybrids promising for deep grain processing, combining high protein (10.3–13.53%) and oil (3.77–5.03%) levels with high starch content (69.02–70.4%) in their grain. Native starch extraction using L. P. Nosovskaya’s method showed that grain starch content in the best 68 hybrids ranged from 70.03 to 71.95% DMB. The collection was ranked according to the main heterotic groups: 57 lines of Iowa Dent, 26 lines of Stiff Stalk Synthetic, and 28 lines of Lancaster. For CMS-S and CMS-C types, 33 and 6 maintainers, and 9 and 8 restorers were selected, respectively. The hybrids were distributed across the following FAO maturity groups for maize: FAO 200–299 (14 hybrids), FAO 300–399 (7), FAO 400–449 (21), and FAO 450–500 (29). Conclusion. Assessing agronomic and breeding prospects of the best 68 hybrids between high-starch maize lines and sterile testers proved their potential for producing native starch to at least 70–72% DMB. Five hybrids were identified as promising for yielding native starch (69.02–70.4% DMB), as well as protein (10.3–13.5% DMB) and oil (3.77–5.03% DMB) by-products during deep grain processing.

Background. High content of organic compounds that worsen nucleic acid purification in aromatic plants, as well as the use of such toxic substances as phenol and mercaptoethanol in many protocols for plant DNA isolation, make it advisable to take the above disadvantages into account when optimizing the DNA extraction technique for the work with essential oil rose plants. Material and methods. Rose accessions from the Crimean Federal University’s Botanical Garden, and those from the collection held by the Research Institute of Agriculture of Crimea were included in the study. DNA extraction was done according to a modified CTAB protocol. Effectiveness of the technique was assessed using spectrophotometry, horizontal electrophoresis, and ISSR-PCR. Results. DNA preparations extracted with the modified technique were well visualized on the electropherogram and demonstrated high spectrophotometric values. DNA content was twice as high in preparations isolated with an extraction buffer with PVP, compared to a PVP-free buffer. The concentration was also higher in DNA extracts from stems than that from leaves. Purity parameters expressed by the absorption ratios at wavelengths A260/280 and A260/230 were again higher for DNA extracts from stems isolated with an enriched buffer, the A260/230 ratio falling within the normal range only in DNA extracted from stems in the presence of PVP. Besides, DNA extracts were effectively purified from proteins without phenol or mercaptoethanol, due to double rinsing with a chloroform/isoamyl alcohol (24 : 1) mixture. Conclusion. Using rose stem tissues as the research material, adding 2% PVP to the extraction buffer, and twofold rinsing with a chloroform/isoamyl alcohol mixture made it possible to obtain DNA extracts with high concentrations and purity indices within normal ranges suitable for the ISSR analysis of essential oil rose genetic diversity.

Background. Soybean is the main supplier of complete vegetable protein and oil. Prior to the emergence of new early-ripening cultivars, this crop was not cultivated in Irkutsk Province. Its introduction into the area requires a detailed study of agricultural practices, primarily the choice of optimal sowing dates. Materials and methods. Soybean cv. ‘Barguzin’, late-ripening under the conditions of Irkutsk Province, and early-ripening accession 15 served as the research objects. Field studies were carried out in 2019–2021. There were four sowing dates, from the first ten-day period of May through the first ten-day period of June. Results and conclusion. Cv. ‘Barguzin’ appeared highly productive under the climate of Irkutsk Province (its average seed yield in 2019–2021 was 2.7 t/ha), but its productivity was significantly influenced by weather conditions and sowing dates (seed yields varied from 1.4 to 3.6 t/ha). The optimal sowing times for this cultivar are the first and second ten-day periods of May. Early-ripening accession 15 was characterized by more stable yield values in the studied years (average productivity was 2.4 t/ha, minimum 1.9 t/ha, and maximum 3.2 t/ha). The preferred sowing times for this accession are the second and third ten-day periods of May. The case study of two soybean genotypes differing in maturation schedules revealed the need for individual selection of sowing dates. It was shown that the early-ripening soybean genotype imposed less stringent requirements on the choice of sowing dates.

Aim. Evaluation of genes polymorphisms frequencies of angiotensinogen (AGT), angiotensin converting enzyme type 1 (ACE1) and angiotensin II receptors type 1 (AGTR1) and type 2 (AGTR2) in patients admitted with coronavirus disease (COVID-19) and its association the severity of severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2). Materials and methods. The study included 100 patients admitted to the hospital with a laboratory-confirmed diagnosis of COVID-19. All patients were identified with alleles and genotypes of polymorphic markers rs4762 of the AGT gene, rs1799752 of the ACE1 gene, rs5186 of the AGTR1 gene and rs1403543 of the AGTR2 gene. The frequencies of each polymorphisms were compared with population. Statistical processing was performed using the Statistica 8.0 software package. Results. In evaluated cohort there was higher frequency of D-allele ACE1 rs1799752 compared to population. Depending on the availability of criteria for the severity of coronavirus infection, 44 (44%) patients were diagnosed with severe, 56 (56%) with moderate course. The groups did not significantly differ in age, gender, cardiovascular risk factors and comorbid pathology. In the groups with severe and moderate course, the same distribution of genotypes and alleles of AGT rs4762, AGTR2 rs1403543 and ACE1 rs1799752 was revealed. For the I/D alleles of the ACE1 rs1799752 gene, a significant deviation from the papulation was found in both the group of severe and moderate COVID-19. In the group with a severe course of the disease, a higher frequency of the mutant C-allele of the AGTR1 rs5186 gene was detected. In the same group, a deviation in the frequency ratio of A and C of the AGTR1 rs5186 alleles from Hardy–Weinberg Equilibrium was found. When calculating the risk of severe COVID-19 in the presence of the C-allele compared with the A-allele, an odds ratio 2.092 (95% confidence interval 1.066–4.108) was obtained. Conclusion. The data obtained suggest that the genes polymorphisms of the components of renin-angiotensin-aldosterone system, namely D-allele of ACE1 rs1799752 and C-allele of AGTR1 rs5186, may make it possible to identify groups of patients predisposed to the development of more severe COVID-19.

Dilated cardiomyopathy (DCM) is a leading cause of heart failure, sudden cardiac death, and heart transplantation in young patients. The causes of DCM are varied and include genetic factors and metabolic, infectious, toxic and others factors. Today it is known that germline mutations in more than 98 genes can be associated with the occurrence of DCM. However, the penetrance of these genes often depends on a combination of factors, including modifiable ones, i.e. those that change under the influence of the environment. About 20–25% of genetically determined forms of DCM are due to mutations in the titin gene (TTN). Titin is the largest protein in the body, which is an important component of the sarcomer. Although titin is the largest protein in the human body, its role in the physiology of heart and disease is not yet fully understood. However, a mutation in the TTN gene may later represent a potential therapeutic target for genetic and acquired cardiomyopathy. Thus, the analysis of clinical cases of cardiomyopathy in patients with identified mutations in the TTN gene is of great scientific interest. The article presents a clinical case of manifestation of DCM in patient with a revealed pathogenic variant of mutation in the gene TTN and reverse left ventricular remodeling of the against the background of optimal therapy of heart failure in a subsequent outpatient observation.

Problem statement. Today, virtual reality technologies are actively and systematically used only in the field of various entertainments, despite their enormous educational potential, which is insufficiently used in the system of educating students. Experimental study is aimed at filling this gap through the introduction of virtual technologies into the process of professional-personal development of students. The goal of the research is to theoretically substantiate and experimentally prove the effectiveness of introducing virtual reality technologies to increase the level of professional and individual development of students in various areas of undergraduate training. Methodology. In the experiment took part 118 students from psychological-pedagogical, socio-cultural and engineering areas of undergraduate training. All participating in the experiment carried out blended learning: some subjects were taught in a classroom in a full-time format, and some disciplines were taught remotely using video conferencing on the Telegram platform and virtual reality technologies on the Mootup and Vive Sync platforms. Results. Virtual reality technologies are an effective means for the holistic professional and personal development of students. In the context of the activity-behavioral and cognitive-reflexive components, the highest rates of increasing the number of students at high and situational levels of development were recorded. The smallest experimental impact was recorded on the motivational-axiological component of students’ professional and personal development. A more detailed analysis of the obtained comparative data after the implementation of the experimental work showed that the author’s development had a more significant impact on students of engineering areas of professional training, compared to students of other undergraduate profiles. Conclusion. The use of virtual communication has made distance learning more attractive and focused for students, greatly facilitating the exchange of information, knowledge, values and views, and making this process more natural, as in real classes.

Introduction. To enhance the quality of power and ensure a consistent electricity supply, this study proposes the utilization of a unified power quality conditioner (UPQC) system integrated with solar photovoltaic (PV) technology. The innovation involves single DC-link connecting back-to-back voltage-compensating components arranged in series and shunt, forming the PV-UPQC. The shunt compensator utilizes energy from a PV array to address harmonics in the load current. The objective is to mitigate voltage dips and spikes by injecting voltage that is either in phase with or out of phase with the common coupling point through a series compensator. The method combines the benefits of generating renewable energy to enhance electrical quality. The goal of the paper is the power quality enhancement of grid-integrated solar PV system. The novelty of the proposed work consists of enhancement of grid-integrated solar PV system with UPQC. The purpose of integrating a UPQC into a grid-connected solar PV system is to enhance power quality by mitigating issues such as voltage fluctuations, harmonics and reactive power imbalance. Methods. The proposed topology is implemented in MATLAB/Simulink with grid-integrated solar PV system with UPQC. Results. Integrating UPQC with a grid-connected solar PV system yields substantial improvements in power quality. This includes effectively mitigating voltage fluctuations and harmonics, resulting in smoother operation and reduced disturbances on the grid. Practical value. The proposed topology has proven to be extremely useful for grid-integrated solar PV system with UPQC applications. References 15, table 2, figures 9.

Introduction. Spark-erosion processing of metals and alloys granules in working liquids is the basis of a several technological processes. Efficiency of energy use in them and parameters of the resulting product largely depend on the accuracy of stabilization and regulation of pulse power in each plasma channel between the granules. To achieve this, until now only the voltage and current of the discharge pulses in the entire layer of granules have been controlled. Problem. The measurement methods, which are used, are not effective enough for monitoring the parameters of individual plasma channels and predicting the size distribution of eroded metals particles at the stage of their formation. The aim of the work is to develop a method for determining the volumes of components of plasma channels in layers of metals granules during their spark-erosion treatment to predict the size distribution of eroded metal particles at the stage of their formation, as well as to simplify the method of spectrometric analysis of the elemental composition of substances surrounding plasma channels for the operational prediction of the chemical composition of resulting products. Methodology. A series of experiments were carried out on spark-erosion processing of Al and Ag granules layers in distilled water. Using a digital camera, images of the plasma channels in them were obtained. Based on the theory of pulsed electrical breakdown of liquid dielectrics, an analysis of the components of plasma channels was carried out. Using the specialized ToupView program, the volumes of equivalent ellipsoids of rotation were determined, approximating the halos of colored radiation likely arising from streamers, as well as the spark cores of plasma channels emitting white light. The shades of the resulting radiation were studied for several metals and working liquids. The obtained data were compared with the known results of spectrometric studies for the same elements excited by similar mechanisms. Results. The theory of discharge-pulse systems for spark-erosion processing of granular conductive media has been developed in the direction of new methods for monitoring the parameters of discharge pulses and predicting the chemical composition and size distribution parameters of eroded metal particles at the stage of their production. An optic-metric method has been developed for determining the volumes of halos and cores of plasma channels. A simplified spectral method for determining the chemical composition of erosion particles based on the shade of the resulting radiation was proposed. Originality. The developed new optic-metric method makes it possible to obtain information about almost every plasma channel, which refines predictions of the size distribution of erosion particles. To implement the method, general-purpose hardware and specialized software that is freely available are used. The developed method of simplified spectral analysis of excited atoms makes it possible to make preliminary predictions of the chemical composition of the obtained erosion particles already at the stage of their formation without the use of expensive specialized equipment. Practical significance. The ratio of the volumes of halos and cores of plasma channels between Al and Ag granules in distilled water was measured. An analysis of the emission spectra of plasma channel halos between Al, Ag and Cu granules in distilled water, Fe in ethyl alcohol, Ni-Mn-Ga and Ti-Zr-Ni alloys in liquid nitrogen, and Ti-Zr-Ni in liquid argon was carried out. Based on spectrometry data, the resulting shades of these radiations were substantiated and their description in the RGB system is given. References 56, table 1, figures 4.

The paper presents the results of an experimental study of the possibility of using a narrow-band semiconductor distributed feedback laser diode used as a source and detector of optical radiation to detect the spectral response from a fiber Bragg grating. The DFB laser “LDI-1550-DFB-2.5G-20/70” from the company “Laserscom”, mass-produced on the Russian market and having standard characteristics, was chosen as the laser diode under study. To sweep the central wavelength of a semiconductor distributed feedback laser diode in the range 1549.5–1552 nm, direct pulse current modulation was used with a frequency of 100 kHz, a duty cycle of 40, and a current value of 1 A per pulse. The radiation reflected from the fiber Bragg grating corresponding to the central Bragg wavelength was recorded as a change in voltage at the anode and cathode of the laser diode due to the photoelectric effect in the laser diode. An experimental assessment of the optoelectronic parameters of a laser diode in photovoltaic and short-circuit modes was carried out: dark current, bandwidth and spectral sensitivity. The evaluation was carried out at a temperature of 25 °C. A measuring circuit has been created to detect the response from a fiber Bragg grating based on direct pulse current modulation and the photovoltaic mode of a semiconductor distributed feedback laser diode. It is shown that the photovoltaic mode of the laser diode is applicable to problems of recording optical radiation. The amplitude-frequency characteristic of a laser diode in the photovoltaic mode was experimentally obtained depending on the forward bias voltage. It is experimentally found that the –3 dB bandwidth is 300 MHz and the maximum sensitivity is 0.1 A/W in short-circuit mode, and the amplitude response is linear in the wavelength range from 1540 to 1560 nm. For the laser diode under study, the reverse branch of the current-voltage characteristic was experimentally obtained and the dark current at zero bias of the laser diode is 12.5 pA. The demonstrated method of FBG interrogation can be used for miniaturization and simplification of optical devices for fiber Bragg grating interrogation. The obtained results may be useful to specialists in fiber optic sensors, system for interrogation and processing signals from fiber optic sensors.

The article describes the possibility of determining the damping coefficient of a support with an elastic ring based on dry friction forces using a special test bench. The forced excitation force is set by the oscillations of a compact speaker installed on top of the oscillating mass through a U-shaped crossbar. The object of the study is a damper with an elastic ring, that is a thin-walled ring with evenly distributed bulges inside and outside, arranged in a checkerboard pattern. The damping coefficient is estimated using a device for simulating a rotor support, an acceleration sensor, an exciter speaker, a controller and a processing station. The amount of damping is estimated according to the width of the peak at the resonant frequency. A comparison is given of the magnitude of the damping coefficient obtained from the analysis of the resonance peak and from the decay rate during the impact experiment.

Precision research and technological equipment, as a rule, is not able to provide its specification characteristics without a high-quality vibration protection system. Active vibration control of an object is provided with the help of an additional source of movement, an actuator. The most promising high accuracy actuators are based on smart materials, such as materials with shape memory, piezoelectric and magnetostrictive materials, electro- and magnetic active fluids and elastomers. Dielectric elastomer is one of the types of electroactive polymers. Actuators based on a dielectric elastomer show high performance in terms of accuracy and speed and operate due to the controllable deformation of the elastomer under the action of a high voltage electric field. The paper provides a comparison of actuators based on sheet and thin film control electrodes. The influence of the quality of the polymer surface and the type of electrodes on the travel range of the actuator and maximum amplitude of vibrations the system can suppress on the basis of a dielectric elastomer is estimated. The formation of the electrode by magnetron sputtering in vacuum makes it possible to create a thin-film layer of copper that covers the elastomer, despite the developed surface. The effect of ion treatment of an elastomer before coating on the quality of the formed electrode is considered. After the ion treatment, the surface of the elastomer acquires a more uniform regular structure. A thin-film electrode layer is formed according to the topology of the elastomer to an accomplished standard.

The article presents the results of optimization of the work process of a single-stage axial turbine in order to increase its efficiency. During optimization, it was necessary to preserve the construction of the original turbine as much as possible. To solve this problem, a parameterization scheme for turbine blades and path contours was developed, taking into account the design and technological limitations. The turbine nozzle blade had an inside baffle. To control the possibility of placing the baffle, a special program was developed that automatically monitors the spatial position of the sections of the blade of the nozzle set. A post-processing program was developed to control the flow parameters at the turbine outlet in height. The efficiency and the vertical deviation of the angle of the outflow from the turbine from the original one were used as optimization criteria. The limitations were the mass flow rate of the working fluid and the total pressure ratio of the turbine. The problem was solved in several stages with different changing variables. As a result of solving the problem, it was possible to increase the turbine efficiency by 0.9%

The article shows how Russian students assess the role of the university as a socialization institution in strengthening the value of family and in their preparing for marriage, birth and raising children. Based on the results of the sociological study conducted in 2023, the authors consider issues related to the content of the educational process and make some recommendations for its optimization in the context of the state demographic policy. The empirical basis of the study consists of (1) the data of the survey of the Russian student youth from Moscow, Saint Petersburg, Kazan, Irkutsk, Orenburg, Pskov, Krasnodar, Perm and Stavropol (N=719); (2) the results of a series of focus groups with students of the Perm State National Research University (N=36). The article aims at identifying the ideas of Russian students about the influence of the university environment on the formation of family values. According to the students, the university educational environment still focuses on solving educational problems: transferring knowledge and skills required for successful employment of graduates and creating favorable conditions for learning - providing students with dormitories, forming scholarship funds. The authors argue that the measures taken to strengthen family values in universities are still insufficient: the students rate the lowest such conditions as taking into account the marital status of graduates when distributing them to workplaces, the availability of psychological and medical services, and the use of different formats for discussing family and childbearing issues. At the same time, some universities update and strengthen the guidelines for maintaining a balance between educational and upbringing activities related to family values. The authors make a conclusion that it is necessary to further improve educational-methodological, organizational, infrastructural and other conditions for maintaining a family-friendly environment at the university.

Aim. To assess the incidence of chronic myocarditis in patients with atrial fibrillation.Material and methods. The study included 145 patients. The majority of patients were male — 118 (81.4%). The median age was 45 (38; 50) years. Most had paroxysmal AF — 60 (41.4%), slightly fewer had persistent AF — 55 (37.9%), 30 (20.7%) patients had long-term persistent AF. All patients underwent radiofrequency ablation of AF and endomyocardial biopsy (EMB) with subsequent histological and immunohistochemical studies (IHC). Morphological verification of myocarditis was performed in accordance with the Dallas criteria modified by the World Heart Federation.Results. Signs of chronic myocarditis were identified in 64 patients (44.1%). The median age of patients with myocarditis was 44 (36.5;49) years, without myocarditis — 46 (38;51) years. In men, myocarditis was detected in 50 cases (42%), in women — in 14 (51.9%). In paroxysmal AF, myocarditis was detected in 25 patients (41.7%), in persistent AF — in 23 (41.8%), and in long-term persistent AF — in 16 (53.3%). The groups with and without chronic myocarditis were comparable when compared based on the main echocardiographic parameters. Regression analysis did not show a significant influence of these factors on the probability of detecting chronic myocarditis in patients with AF. In patients under 30 years of age, there were no signs of inflammation without the presence of myocardial fibrosis, while the maximum stage of fibrosis occurred in patients from 31 to 40 years of age. The stage of fibrosis did not depend on gender. More often than others, a combination of enterovirus and herpes virus type 6 was detected in biopsy specimens. In patients over 51 years of age, biopsies generally did not express any virus on immunohistochemical studies.Conclusion. Chronic myocarditis in patients with AF was significantly more often detected in younger patients (up to 50 years inclusive), and was more common in women, as well as in the presence of a long-term persistent form of AF, regardless of gender. Detection of myocardial fibrosis signs is more typical for young patients with the maximum stage at the age of 31 to 40 years.

Aim. To investigate the state of the platelet and plasma components of hemostasis in patients with myocardial infarction with non-obstructive coronary arteries (MINOCA).Material and methods. The study included 42 patients with non-ST-segment elevation myocardial infarction (NSTEMI): MINOCA (n=24) and MI-CAD (n=18). Platelet aggregation ability in response to activation was evaluated using Solar AP2110 and LASCA aggregometers. Platelet functional activity and calcium signaling were assessed using flow cytometry methods. The plasma component of hemostasis, in addition to routine coagulation tests was evaluated using the global coagulation test "Thrombodynamics". The control groups for tests consisted of healthy volunteers.Results. When analyzing the ability of platelets to form aggregates by the aggregometry tests, it was found that platelets in the MINOCA group formed aggregates significantly worse upon ADP stimulation at various concentrations compared to the MI-CAD group. However, when platelets were stimulated with collagen, the opposite effect was observed: in the MI-CAD group, there was a noticeable decrease in aggregate formation in terms of light scattering amplitude compared to the MINOCA group. Flow cytometry using the functional platelet activity test protocol revealed that both groups showed a significantly increased platelet size after activation, reduced platelet granularity) both at rest and upon activation, significantly decreased number of procoagulant phosphatidylserine-positive platelets, and reduced dense granule release upon activation compared to healthy volunteers. The calcium signaling test showed a weakened calcium release in response to ADP in the MINOCA group compared to the MI-CAD group. In the study of the plasma component, no significant differences between the groups or deviations were found according to both routine tests and the "Thrombodynamics" test.Conclusion. Platelet activity did not differ significantly between the MINOCA and MI-CAD groups; however, in the MINOCA group, platelet activity was lower in some tests compared to the MI-CAD group. In the study of the plasma hemostasis component, normocoagulation was recorded in both groups.

The article describes a clinical case of BRAHH syndrome in a patient with arterial hypertension and permanent atrial fibrillation (AF). The patient took perindopril 10 mg, indapamide 2.5 mg, amlodipine 10 mg, bisoprolol 2.5-5 mg daily. She was admitted to a hospital complaining of severe weakness, a heart rate decreases to 38 beats per minute against the background of high blood pressure. During the examination, she was diagnosed with complete atrioventricular block against the background of AF, stage 4 chronic kidney disease and severe hyperkalemia (potassium 8.7 mmol/l). The patient was prescribed treatment aimed at eliminating hyperkalemia, and temporary pacing was established. Against this background, her condition improved, and the complete atrioventricular blockade was resolved. This clinical example meets the criteria of BRAHH syndrome, since against the background of taking an atrioventricular node blocker in a small dose, a patient with chronic kidney disease and severe hyperkalemia developed complete atrioventricular block against the background of AF, accompanied by high blood pressure.

Cardiology, like other branches of medicine, is increasingly faced with the need not only to optimize patient treatment, reduce financial costs, but also to improve long-term outcomes. The volume of information required for such tasks is significant, and a doctor’s time is severely limited. Additional software capable of processing large amounts of data in a short period can assist doctors. Clinical decision support system (CDSS) is a type of software that, based on numerous clinical characteristics, provides doctors with information on the most likely diagnosis, patient risk profile, most suitable therapy, and more. Nowadays, CDSS finding more and more applications in cardiology and cardiovascular medicine. The experience of its clinical use has also been accumulated in the Russian Federation. The problem of the correct choice of anticoagulant therapy remains relevant in clinical practice. In addition, despite the proven benefit of prescribing anticoagulants in patients with atrial fibrillation (AF) at a high risk, the frequency of "underprescription" of oral anticoagulants (OACs) remains relatively high. The introduction of a strategy for a personalized approach to the selection of anticoagulants, based on individual patient characteristics, can significantly improve adherence to clinical guidelines and, as a result, reduce the risk of thromboembolic complications. This article discusses the positive and negative aspects of using CDSS in the management of patients with AF, highlighting the main limitations when using them in conditions close to real clinical practice.

The investigations were made on Moringa oleifera, a miraculous plant having an excellent nutritive and pharmaceutical importance, under saline and drought conditions. The effect of salt stress on morphological parameters under 200 mM NaCl concentrations including height of plant, number of leaves, root length along with their biomass were examined with the interval of 30 and 60 days, which significantly resulted in a decreased height of plant, number of leaves and their biomass. Hence, growing Moringa oleifera under highly saline conditions may not be recommended but it can be grown under moderate saline conditions. Growth, total biomass and physical parameters of the investigated plant were also analyzed under drought conditions. Treatment set was irrigated with 20% less than the total field capacity while control was irrigated with 100% according to total field capacity. Some growth parameters were measured after 30 and 60 days including stem height, number of leaves, root length and fresh and dry weights of stem, root and leaves. Drought stress surprisingly gave good results after 60 days of an increased tolerance with growing age, hence it showed that. It is a potential plant species to be planted in arid and semi-arid areas because of its drought loving nature.

The article considers the research on the development of bread with added new ingredient – French willow. French willow or Ivan-tea is widespread throughout the Ural region. It contains all the vitamins and microelements necessary for the body, which help to reduce oxidative processes. The components of Ivan-tea have immunostimulating properties, they affect the processes of hematopoiesis and the activity of vitamins in the body and are of great importance in blood diseases, atherosclerosis, and some types of tumors. The development will expand the range of bread offered to the population in stores. And create new types of bread, including functional ones [1, 2, 3]. The production of bread samples consisted of the following stages: raw material preparation, ingredient addition, dough kneading, dough maturation, kneading, baking, cooling and product inspection. Five bread samples were produced and analyzed. Samples No. 1 and No. 2 were made using live sourdough, while samples No. 3 and No. 4 used baker's yeast. The control bread sample was made in accordance with GOST R 31807-2018, which is valid in the Russian Federation. Bakery products from rye flour and a mixture of rye and wheat flour. General specifications. French williw infusion was added to four samples (No. 1, No. 2, No. 3, and No. 4) in amounts of 100 and 200 ml. The organoleptic properties of all samples were assessed according to GOST 31807-2018. The physicochemical properties included porosity (GOST 5669-96), acidity (GOST 5670-96), and humidity (GOST R 58233-2018). The research showed that the best sample was wheat-rye bread (No. 2), prepared with sourdough and 200 ml of fireweed infusion. The appearance of the product corresponded to the baking form - tin. The surface was without cracks and tears. Color was light brown. Taste and smell were typical for this type of product with notes of fireweed. Porosity was developed, without compaction. Traces of unmixed particles were not found. The humidity of the experimental sample No. 2 was 47%, the acidity index was 0.5° higher than the norm and was - 3.5°. The porosity index was 68.82%. Thus, it can be assumed that the French willow infusion affects the physicochemical properties of bread products (increasing porosity by 0.82%). And it affects the organoleptic properties of the samples, improving the taste and appearance of the product.

Cereals and legumes have a porous structure, being an inert carrier during mass exchange processes; they contain voids in the form of pores and stomata, on the surface of which there are foreign extractable substances in a solid state, in particular, trypsin and urease inhibitor proteins. These substances, due to their harmful effect on the final product, are subject to removal by extraction. One of the methods for inactivating anti-nutritional substances is ultrasonic treatment of soybeans.The article discusses the technology of inactivating inhibitors that worsen food and protein value of vital soybeans. The developed technology was aimed at dissolving a solid substance - soybean inhibitor protein in a liquid, carried out due to molecular diffusion, intensifying the processes of mass transfer and mass return. When creating a suspension by mixing the solid (crushed soybean grain) and liquid (water) phases, water molecules, which are a solvent, penetrate under the action of capillary forces into the pores and stomata of the treated body, begin to penetrate. On this basis, technological directions of research have been established, a sequence has been built and the physical essence of operations has been substantiated, consisting of four main ones - grinding soybean grain, creating a “water-soybean” suspension, ultrasonic (US) treatment of soybeans, drying. As a result of the conducted research, optimal technological parameters have been established for intensifying the process of inactivation of anti-nutritional substances contained in native soybeans: the degree of grinding of soybean grains is 0.5-1.0 mm; the ratio of water-soy suspension (1:1). The efficiency of the developed technological process will be factors affecting the quality of the resulting products, the duration and energy intensity of the process, resource conservation, simplicity of design and low cost of manufacture, the possibility of automation.

Background. The cytoskeletal protein β-tubulin class III (Tubb3) is associated with tumor resistance to taxanes and vinca alkaloids, as well as with the metastatic potential of neoplasm, however, data from immunohistochemical analysis of Tubb3 expression in non-small cell lung cancer (NSCLC) tissue are few and contradictory. Purpose. Characterization of the level and intensity of Tubb3 expression in NSCLC tissue and analysis of the identified parameters correlation with clinically significant characteristics of the disease. Methods. Quantitative assessment of the level and intensity of Tubb3 expression in 120 surgical samples of NSCLC was carried out by immunofluorescence method associated with flow cytometry. Primary rabbit monoclonal antibodies specific to Tubb3 and secondary anti-rabbit antibodies conjugated with fluorescent dye DyLight650 (ab98510, UK) were used. The expression of the marker was assessed by two parameters: the level of expression measured as the percentage of the cells expressing Tubb3 and the intensity of expression in conventional units (CU) represented as the ratio of the geometric mean fluorescence intensity in the experimental and control samples (cells incubated with secondary antibodies only). Results. 1. Tubb3 expression was detected in all NSCLC samples studied. The median level and intensity of Tubb3 expression was 30.5% and 2.0 CU with significant differences (up to 10 times) in the quantitative values of both parameters in different patients. 2. The distribution of the studied tumors in terms of the level and intensity of Tubb3 expression differs from normal (P

Aim. Justification of the optimal dosage regimen regarding the efficacy and safety of Eladis®, film-coated tablets, 10 mg (Valenta Pharm JSC, Russia) in comparison with placebo in patients with non-productive cough due to acute respiratory viral infection (ARVI). Material and methods. A double-blind placebo-controlled randomized multicenter study was conducted in which 141 patients with non-productive cough on the background of acute respiratory viral infection took part. The study participants were randomized into 4 groups. Patients from the first group took 1 tablet of Eladis® 2 times a day (daily dose of 20 mg); patients from the second group took 2 tablets of Eladis® 2 times a day (daily dose of 40 mg); patients from the third group took 4 tablets of Eladis® 2 times a day (daily dose of 80 mg). Patients in the fourth group received placebo tablets in quantities corresponding to the first three groups. For this purpose, 3 subgroups of 12 people were allocated within group 4. The duration of therapy was 7–14 days. The effectiveness of therapy was assessed based on monitoring the frequency of cough attacks, changes in values on the day and night cough scales, a digital rating scale (DRS), and the time to achieve clinical cure of cough and ARVI symptoms was also taken into account. The safety of the studied drug was assessed based on monitoring of vital signs, laboratory parameters, and electrocardiogram (ECG) data. The number and severity of adverse events (AEs) and serious adverse events (SAEs) were assessed. Results. Analysis of data on the primary endpoint (reduction of cough attack frequency by ;)50% by the 5th day relative to the 1st visit) showed the superiority of Eladis® over placebo at a daily dose of 80 mg (P=0.00003). At the same time, the proportion of patients who reached the primary endpoint by day 5 when receiving Eladis® at a dose of 80 mg per day was 71.43%, while in the combined placebo group it was 22.22%. Conclusion. Evaluation of various dosage regimens of Eladis® has shown that a daily dose of 80 mg is optimal for the treatment of non-productive cough in patients with ARVIs, as it provides the greatest effectiveness with a safety profile comparable to other studied doses.

Purpose of the study. To study the effect of prophylactic antibiotic therapy on the course of the postoperative period in patients undergoing endovascular closure of patent foramen ovale (PFO). Material and methods. The study included 276 patients who were submitted to endovascular closure of PFO. The follow-up duration was 12 months. Depending on prophylactic antibiotic therapy the patients were divided into 2 groups - those who did not receive and those who received prophylactic antibiotic therapy (115 and 161 patients, respectively). Results. Cephalosporins were prescribed to patients as prophylactic antibiotic therapy, and in the presence of allergic reactions they were replaced by glycopeptides. In total, patients received 1 to 2 drugs per prophylaxis course. Postoperative complications were present in 37 (22.98 %) patients who received prophylactic antibiotic therapy and 12 (10.43 %) who did not receive prophylaxis (p = 0.007). Access site complications were the most identified, but they were not inflammatory in nature. An elevation of body temperature over 37.1 °C was observed in 19 (11.80 %) patients receiving prophylactic antibiotic therapy compared to 5 (4.35 %) who did not receive prophylaxis (p = 0.03). Meanwhile, marked leukocytosis was absent in both groups. The duration of body temperature elevation did not differ between the groups. They also had a longer duration of hospitalization, 7.00 [6.00; 8.00] bed days, compared to 6.00 [4.00; 7.00]. Discussion. It has been suggested that hyperthermia may be associated with prophylactic administration of antibacterial drugs causing death of persistent microflora. In patients who did not receive prophylactic antibiotic therapy, their concentration was lower, which did not lead to an increase in body temperature. Since there are no indications on the necessity of prophylactic antibiotic therapy before endovascular closure of PFO in clinical recommendations, it is suggested to evaluate its appropriateness for each patient individually.

Purpose: to analyze the effectiveness of transferring open-angle glaucoma patients to therapy with a preservative-free fixed combination of a carbonic anhydrase inhibitor and a prostaglandin analogue. Material and methods. The study involved 58 people (24 men and 34 women), averagely aged 61.2 ± 7.1, diagnosed with stages II–III POAG and degree of compensation a-b (according to the Nesterov-Bunin classification). The patients were divided into three groups. Initally, Group 1 (22 people, 30 eyes) had received monotherapy with a beta-blocker (timolol), Group 2 (20 people, 27 eyes) had received monotherapy with the original prostaglandin analogue drug (latanoprost), and Group 3 (16 person, 19 eyes) had had therapy with the original drug, namely a fixed combination of a beta-blocker (timolol) and a prostaglandin analogue (latanoprost)). All patients were prescribed antihypertensive therapy with a fixed combination of a carbonic anhydrase inhibitor (dorzolamide) and a prostaglandin analogue (latanoprost) — the drug Dorzoprost, which was administered according to the standard regimen. We assessed the dynamics of intraocular pressure (IOP), perimetric indices, morphometric indicators of the optic nerve, tolerability of the drug regarding the effect on the ocular surface and safety regarding potential systemic side effects. Results. After 1 month of therapy, IOP significant decreased by 7.5 mm Hg (32 % of the initial value) in Group 1, by 3.7 mm Hg (19 %) in Group 2, and by 1 mm Hg (5 %) in Group 3. At month 3 and month 6, the hypotensive effect stabilized with respect to the previous control measurements (taken at 1 month) in all three groups. The morphometry of the optic nerve and the dynamics of perimetric indices showed no progression of glaucomatous optic neural opticopathy and no local or systemic undesirable effects of the therapy that could affect the compliance of the treatment. Conclusion. The Dorzoprost drug has demonstrated a high hypotensive efficiency when transferring patients from various basic therapy regimens and can be considered the drug of choice for patients with symptoms of keratoconjunctival xerosis and concomitant organ pathology requiring the use of systemic β-blockers.

Objective. Currently, for the development of Valanginian and other deeplying (compared to the Cenomanian) deposits, low-temperature separation (LTS) units with turboexpander units (TDA) are used, and the booster compressor station (BCS) is one of the key technological facilities necessary to ensure the efficient operation of promising technologies for gas preparation using the NTS method with TDA turboexpander units for transport. The purpose of the study is to substantiate optimal operating modes, as well as to develop measures for the technological development of a booster compressor station based on the use of methods for modeling the operation of centrifugal compressors, gas pumping units and, directly, the booster compressor station. Method. The following methods were used: the method of reduced characteristics, the refined method of reduced characteristics, and the two-dimensional approximation method. The basis of the mathematical model of the research objects is the gas-dynamic characteristics of centrifugal compressors. Result. The results of a comparison of methods for modeling HDC (reduced characteristics method, refined method of reduced characteristics, two-parameter approximation method) of high-pressure pulp and paper mills are presented using the example of a pulp and paper mill with a pressure ratio of up to 1.9...2.1, intended for equipping gas compressor units with a unit power of 16.0 MW as part of field operations. DKS. An analysis and comparison of the obtained modeling results with actual data was carried out. Conclusion. The accuracy of the mathematical model of the GDC PPM as part of the field booster compressor stations that ensure the operation of NTS installations with TDA determines the accuracy of the assessment of energy consumption and the technical condition of the gas pumping units, as well as calculations of future operating modes of gas pumping equipment, which are carried out to solve a number of applied problems, including determining the timing of equipment modernization or reconstruction of the station.

Objective. The article explores a way to improve the security of a corporate network by using the technique of spoofing sender addresses when forwarding packets between computers. Method. Network traffic analysis methods, network protocol level programming and data packet processing algorithms are used. Computers on the network are connected to one server. When sending packets between computers in the network, the sender's address is replaced, and when a packet is received by another computer, the packet is disassembled, and the real sender's address is inside it. Result. The method allows for effective protection of a corporate network from attacks associated with the forgery of addresses of data packet senders. Conclusion. Using the sender address substitution technique when transmitting data in a corporate network is an effective way to increase security and protection from external threats. Further research is aimed at developing complex and reliable methods for protecting networks.

RELEVANCE. We present the results of preclinical testing of an upgraded portable AR­1 gas flow analyzer produced by JSC Krasnogvardeets (Saint Petersburg) in order to create import-substituting technologies and develop practical and scientific spheres of healthcare.THE AIM OF THE STUDY. was to substantiate the possibility of clinical use of the upgraded device, originally intended for testing ventilators and inhalation anesthesia devices, as a monitor of the external respiration apparatus function.MATERIAL AND METHODS. The preclinical study of the upgraded gas flow analyzer was conducted at the V.A. Almazov National Medical Research Center in comparison with devices for conventional (Bellavista 950, Berner Ross Medical, Russia) and high-frequency (IPV-2C, Intrapulmonary Percussive Ventilation, Percussionaire Corporation, USA) mechanical ventilation in volume- and pressure-controlled modes using an artificial lung. The following reflected spirometry parameters were compared: tidal and minute volume under inhalation and exhalation, respiratory rate, maximum and minimum flows, peak and positive end expiratory pressure, fraction of inspired oxygen.RESULTS. A high accuracy of parameter matching shown by the gas flow analyzer and ventilators was demonstrated.CONCLUSION. The upgraded model of the gas flow analyzer can be used clinically as a portable spirometer at the prehospital and hospital stages.

Endovascular thrombectomy (ET) effectively and safely recanalizes the occluded artery and restores the ischemic area in patients with acute ischemic stroke (IS), improving the clinical prognosis of stroke in the anterior and posterior circulation system, expanding the time therapeutic window from no more than 6 to 24 hours, greatly increasing the chances of functional independence and survival. However, some patients develop an unfavorable postoperative outcome, complications and “ineffectiveness” of revascularization. The thrombectomy result depends not only on the patient selection criteria, timing and success of the procedure, but on many other factors as well. Despite the advances in stroke treatment, the issues of neuroimaging and patient selection for ET remain relevant; the pathophysiological mechanisms of the influence of some factors on the effectiveness of the procedure are not completely clear; the causes of “uneffective” revascularization, unfavorable outcome and mortality after ET are unclear. An analysis of global experience in treating ischemic stroke with ET showed the heterogeneity of the patient selection criteria, clinical and neuroimaging variables, prognostic factors and treatment outcomes, which makes it difficult to draw a general conclusion and requires further targeted research. The article discusses the issues of patient selection, pathophysiological mechanisms of the influence of some risk factors on the outcome of ischemic stroke and the causes of unfavorable outcome and death after ET.