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Protective forest strips are the basis of the environmental frame on sparsely wooded areas. To create such highly productive objects, the selection of the corresponding gene pool is required. Plus trees the representatives of this gene pool. The aim of the research is to develop criteria for highlighting plus trees for protective forestry, focused on the creation of plantings, the main parameter of which is the working height. 16 trial areas of cherry oak (Quércus róbur L.), black locust (Robinia Pseudoacia L.), green ash (Fraxinus lanceolata B.), European ash (Fraxinus excelsior L.), thorney locust (Gleditschia triacanthos L.), walnut (Juglans regia L.) have been laid out. A continuous recalculation of heights in 100–142 individuals have been recalculated on each trial area and their statistical indicators have been determined. The height of the trees had a normal or close to it statistical distribution at the studied objects. Data processing was carried out using the Stadia 8.0/Prof licensed program for Windows. The method of selecting plus trees has been proposed, the height of which should exceed the average one by 25% or more. A comparison of the proposed and well -known method has revealed that the proposed one can increase the breeding differential with instrumental selection by 48,25–53,78%, and with eye-instrumental selection by 31,15–41,39%. Criteria for trees of various selection categories have been developed. Due to different conditions, it is recommended to highlight plus trees separately in the extreme and mid-protective forest strips. With breeding inventory, it is also necessary to take into account the sanitary condition of the trees.

Walnut (Juglans regia L.) is a particularly significant plant for humans in terms of its useful properties and in the Russian Federation it can be attributed to the most valuable introducers for forestry and horticulture. It is grown in many countries of the world and the area of its cultivation is constantly expanding. Breeding species for food purposes requires a selection assessment of the quality of its fruits. For this, at different times, appropriate methods were developed and the existing gene pool of the culture was assessed. By now, new varieties and forms have been bred and brought, which also need to be evaluated for rational use for scientific and practical purposes. The descriptions of varieties and forms of walnut often contain insufficient information about all selected traits, which requires the development of new models to assess the variety fund using incomplete data. The purpose of the research is to develop the missing models of selection evaluation using incomplete data and evaluate the world walnut variety fund in terms of fruit quality, to identify varieties and forms of the highest quality category. The well-known methodology and new models have been used for the evaluation. When developing evaluation models for incomplete data, a database of 50 varieties evaluated for all indicators has been used. Using the method of excluding individual indicators, the corresponding multiple regression models have been calculated on its basis, taking into account a different combination of features. The licensed programs Stadia 8.0, Microsoft Excel have been used in the research. The proposed models provide an estimate with an average error of ± 0.48 – 3.72 points at R2 = 0.63 – 0.99. A promising walnut gene pool from 23 countries has been assessed, 69 representatives with fruits of the breeding category of the highest quality identified, and a list of them compiled. Of the 512 ancestors, 19.73% had the selection category of fruits of the highest quality, 62.88% of high-quality fruits, 16.80% of ordinary fruits, and 0.59% of varieties and forms of low-quality fruits.

In order to prevent crop yield losses from the most dangerous and economically important pathogenic organisms, it is necessary not only to monitor the virulence gene pool, but also to study the nature of pathogen variability and determine the potential for the emergence of new genes and races. This requires centralized collections of fungal cultures characterized by a set of stable strains to provide for phytopathological, immunological, breeding, genetic, toxicological, parasitological and other studies. The State Collection of Phytopathogenic Microorganisms of the ARSRIP is the State Depository of Phytopathogenic microorganisms that are non-pathogenic to humans or farmed animals. Currently, it has more than 4,500 accessions of plant pathogenic strains of fungi, oomycetes, bacteria, viruses, phytoplasmas, and the collection is updated annually. For this purpose, the study of the inter- and intraspecific genetic diversity of genus Fusarium was carried out in agricultural systems of the Krasnodar Territory. In 2020, the State Collection of Phytopathogenic Microorganisms was supplemented with 13 strains of Fusarium fungi isolated from tissues of winter wheat plants collected in several locations of the Krasnodar region. The complex of Fusarium fungi revealed on winter wheat usually included Fusarium oxysporum, F. culmorum, F. lolii, F. graminearum, F. fujikuroi, F. sporotrichioides, etc. The effect of the preceding crop on the frequency of Fusarium species isolated from winter wheat was observed. After series cloning of collected isolates, 21 strains of different fungal species characterized by stable morphology traits and known pathogenic and phytotoxic properties were selected for collection replenishment. Significant differences in pathogenic activity were revealed between fungi belonging to either the same or different species; the manifestation of this activity varied from the absence of any effect of spore suspensions on seedling development to a complete inhibition of their growth. The phytotoxic activity towards wheat seedlings varied from medium to high. Species possessing a high intensity of phytotoxic activities are the most dangerous for wheat, since they promote accumulation of dangerous phytotoxins in plant tissues.

Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new varieties and hybrids that are resistant to low temperatures is an urgent problem. Cellular biotechnology is aimed at solving this problem using methods of clonal microreproduction, cell selection, somatic hybridization, etc. For rapid reproduction and obtaining high-quality planting material, biotechnology methods are used, in particular, clonal micro-propagation. However, in this technology there are difficulties associated with poor adaptation of microclones to ex vitro conditions. This fact introduces an additional requirement for the selection of optimal rooting modes in vitro and ex vitro adaptation of microclones.Material and methodology. The aim of the work was to study the influence of cultivation conditions on in vitro rooting and ex vitro adaptation of I. batatas (L.) microclones. The object of the study was sweet potato microgears propagated in vitro. I. batatas micro-gears were cultured in vitro on a Murashige-Skug medium, differing by the type of auxins. The influence of red (R) and far red (FR) light on the shoots rooting in vitro and the adaptation of microclones ex vitro was studied.Results. It has been experimentally established that the cultivation of micro-gears on a medium containing indolyl butyric acid at a concentration of 0.5-1 mg/l and under conditions of illumination by LED lamps of red and far red light in equal amounts leads to the production of microclones with a well-developed root system and vegetative biomass. The use of an aeroponic installation at the last stage of clonal micro-propagation makes it possible to obtain high-quality planting material that can adapt well to open ground conditions.

trichothecenes and their derivatives have recently attracted much attention of researchers with respect of their potential role in medicine, including for the treatment of different types of cancer. The purpose of the study was to investigate the cytotoxic effect of Fusarium trichothecene on human breast cancer cells, human skin fibroblasts and embryonic kidney cells in vitro. Material and methods. Based on the Mtt assay, the cytotoxic effect of trichothecene on cell cultures was determined. Evaluation of morphological changes in cell cultures under the influence of trichothecene was performed by light microscopy. Results. Fusarium trichothecene was found to exhibit a dose-dependent toxic effect on cell lines in the range 1 nM to 1000 nM. the most pronounced cytotoxic effect of trichothecene was observed in human breast cancer cell line (IС50 94.72 ± 4.1 нМ). Lower doses of trichothecene led to a change in the size, shape of human breast cancer cells, human skin fibroblasts and embryonic kidney cells, and loss of contact between them and their isolation. the degradation of cell membranes, formation of unformed cell aggregates and fragments were observed in higher doses of trichothecene. Conclusion. Fusarium trichothecen is a biologically active compound and is less toxic to the normal than to the cancer cell lines, therefore, further studies of this agent are needed.

Aim. Taking into consideration one of the most important environmental problems of agriculture, contamination of the soil with residues of pesticides, in particular herbicides, we have investigated the technology of developing complex substances and including them in the composition of antidotes that protect crop plant from the residues of herbicides.Methods. To prepare the complex preparations we have proposed methods of mechanochemistry, namely co-grinding of several components having fungicidal, insecticidal, growth-regulatory and antidotal properties.Results. These complex compositions have been used for seed protection and showed a wide range of biological activities demonstrating synergism.Conclusion. Developed complex compositions have a synergistic effect (fungicide, plant growth regulator, an antidote against the residues of herbicides).

Primary receptacles improve profitability legumes are limiting demonstrations and acts of plant diseases and pests. Pathogens are 25-50% lower yield of soybean, chickpea, beans, peas. Pests focally up to 87% of viable seeds sown reduce the number of plants per 1 ha. Only effective protection against disease and estimates of crop production can increase the average profitability of legume crops by 15-30%. Livestock is very important, but in the Southern Urals requires real support for its production with a positive balance (in the calculations with a deviation of 5%). The most important resource in our opinion may be a reduction in price of fodder. Thus, legumes are sought for animal protein. Soybeans, chickpeas, beans, peas universal culture and the possibility of their use in the food balance for a healthy diet of ordinary people engaged in recreational and other sports niche expands further improve the profitability of their production. Regulation of the balance of the distribution of food and feed produced grain legumes allows fine regulation of the cost of fodder for a particular type of livestock activities. Phytosanitary capabilities , the balance of influence of legumes on arable land, also requires a fine regulation of these processes. Obtaining long-term public support for this production is unlikely in the WTO because actual search for ways to improve the profitability of production of agricultural technologies. In our view, a comprehensive approach taking into account the capacity of local markets for crop production. Such activity can act as a guaranteed quality of agro-technology and animal products from local resources specific zonal conditions of production.

Varieties of winter wheat of world selection were studied in the conditions of South Ural. The conducted researches showed that in conditions of a steppe zone of South Ural possibly receiving more than 5,0 t of grain of winter wheat. For a steppe zone of South Ural four effective grades of winter wheat are defined: Kolos 47, Nika, KNIISH 756, Mironovskaya 64.

Efficiency of beekeeping is defined by breed of bees and melliferous herbs. In the conditions of sharply continental climate of South Ural, duration of success of beekeeping is provided with breed of bees.

Pests of the order Lepidoptera - South barn and Grain moth moth - one of the most dangerous pests of grain mass. The data obtained show that the technology (seed disinfectants and quality) of spring wheat affects the damage Plodia interpunctella Hb. and Sitotroga cerealella Oliv.