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Para avaliar o efeito do probiótico sobre a resposta imunológica de frangos de corte desafiados com Salmonella Minnesota (SM), 60 frangos foram divididos em três grupos: CN- (controle negativo) aves que não foram inoculadas com SM, CP- (controle positivo) aves inoculadas com SM e Probiótico- aves suplementadas na ração com probiótico composto de Lactobacillus acidophilus, L. plantarium, L. rhamnosus, L. bulgaricus, Enterococcus faecium, Streptococcus thermophilus e Bifidobacterium bifidum e desafiadas com SM. Aos 14 dias foi realizada a inoculação com SM e aos 7 e 35 dias foram quantificadas células caliciformes, CD4+ e CD8+ na mucosa intestinal do íleo e ceco. Aves suplementadas com probióticos aos 7 dias de idade apresentaram aumento significativo (P≤0,05) de células caliciformes e CD4+ no íleo e de células CD8+ no ceco. Aos 35 dias houve aumento significativo (P≤0,05) das células CD8+ nas aves inoculadas do CN e Probiótico. A utilização de probióticos proporcionou redução significativa (P≤0,05) da contagem de Salmonella sp.To evaluate the effect of probiotics on the immune response of broiler chickens challenged with Salmonella Minnesota (SM), 60 chickens were divided into three groups: CN - Birds that were not inoculated with SM (negative control), CP - birds inoculated with SM (positive control) and Probiotic- birds supplemented with probiotic consisting of Lactobacillus acidophilus, L. plantarium, L. rhamnosus, L. bulgaricus, Enterococcus faecium, Streptococcus thermophilus and Bifidobacterium bifidum in diet and inoculated with SM. At 14 days the birds were challenged with SM, and at 7 and 35 days were quantified goblet cells, CD4 + and CD8 + intestinal mucosa of the ileum and cecum. Birds supplemented with probiotics, at 7 days of age showed a significant increase (P≤0.05) of goblet cells in the ileum and CD4 + and CD8 + cells in the cecum. At 35 days there were significant (P≤0.05) of CD8 + cells in birds inoculated on CN and Probiotic. The use of probiotics provided a significant reduction (P≤0.05) of Salmonella sp. counts.

The present study was designed to compare the ability of different probiotics, applied in feed, to reduce Salmonella colonization and interfere with the cellular dynamics in the intestinal mucosa of broilers challenged with Salmonella enterica enterica sorovar Minnesota (SM). The following probiotics were used: A composed of Bacillus subtillis CCT 7711; B containing Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Enterococcus faecium, Streptococcus thermophilus, and Bifidobacterium bifidum; C consisting of Bacillus subtilis DSM 5750; and D with Enterococcus faecium. All tested probiotics were able to significantly reduce the Salmonella counts in cloacal swabs assessed at 48 h after inoculation when compared with the positive control. All probiotics were able to reduce the Salmonella counts in the cecum when compared to the positive control at 35 days. None of the tested probiotics reduced the Salmonella counts in the crop of broilers at 35 days of age. All probiotics induced an increase in the CD4 + and CD8+ cell counts in the cecum mucosa relative to the negative control group at 7 days of age; only probiotics C and D, reduced significantly the CD8 + cell counts in this same tissue of broilers at 35 days compared to positive control group. The results from this study indicated that the tested probiotics presented different effects on the reduction of SM counts and affected the cell dynamics in the cecum mucosa of broilers differently. [ABSTRACT FROM AUTHOR]