Search

Estudou-se a cinética de crescimento de Borrelia burgdorferi, por um período de 3 meses, utilizando os seguintes oito meios de cultivo : (1) BSK adicionado de soro de coelho, (2) BSK adicionado de soro de suíno, (3) BSK adicionado de soro de suíno + 5 fluorouracil, (4) PMR, (5) CTB, (6) Dubos, (7) Caldo Brucella e (8) BHI. Todos os meios foram preparados assepticamente e mantidos em tubos de ensaio com capacidade para 10 ml. Para cada meio, o inoculo foi padronizado para conter no início 10² espiroquetas para cada 0,1 ml de cultivo. O monitoramento do crescimento foi feito contando-se o total de espiroquetas em 0,1 ml do meio entre lâmina de microscopia e lamínula com dimen sões de 10x30mm, tendo sido utilizado microscópio de campo escuro. A contagem foi realizada durante 14 semanas, tendo sido diária nos primeiros 12 dias e semanal a partir desta data. Houve crescimento de B. burgdorferi em todos meios testados, com melhor performance para três deles: BSK adicionado de soro de coelho, BSK adicionado de soro de suíno + 5 fluorouracil e meio CTB. Observou-se crescimento de B. burgdorferi a partir da 4ª semana, atingindo o platô de crescimento entre a 8ª e 12ª semanas, quando começou a exaustão do meio de cultivo. Formas císticas de B. burgdorferi foram observadas em todos os meios testados.The cinetic of growth of Borrelia burgdorferi was studied during a 3-month period, using the following 8 culture media: (1) rabbit serum BSK, (2) swine serum BSK, (3) swine serum BSK+5 fluorouracil, (4) PMR, (5) CTB, (6) Dubos, (7) Brucella broth and (8) BHI. All media were prepared aseptically and were maintained in culture tubes of 10 ml capacity. For each medium, the inoculum was standardized to contain initially 10² spirochetes for each 0.1 ml of culture. The growth was monitorized by counting the total number of spirochetes in 0.1ml of medium in a dark field microscope, using a 10x30 mm cover slip. For the first 12 days, counting was done each 24 hours, and afterwards once a week during 14 weeks. There occurred growth of B. burgdorferi in all tested media, with the best performance of three of them: BSK with rabbit serum, BSK swine serum + 5 fluorouracil, and CTB medium. Growth of B. burgdorferi was seen from the 4th week on, reaching its maximum within 8-12 weeks, depleting the culture medium after this time. Cystic forms of B. burgdorferi were observed with all tested media.

The aim of this research was to determine the occurrence of anti Brucella abortus, anti B. canis and anti Leptospira spp. antibodies in foxes (Pseudalopex vetulus). Sixty road-killed wild foxes from semi-arid of Paraiba state, Northeastern region of Brazil, were used. For the detection of anti B. abortus antibodies, the buffered plate agglutination test (BPAT) was applied as a screening method and the 2- mercaptoethanol test as a confirmatory method. For serological diagnosis of infections by B. canis and Leptospira spp., the agar gel immunodiffusion test (AGID) and the microscopic agglutination test (MAT) were used, respectively. All samples were negative for anti B. canis and anti Leptospira spp. antibodies. Of the 60 foxes tested, 16 (26.6 %) were positive for anti B. abortus antibodies at BPAT, four (6.7%) samples were confirmed in the 2-mercaptoethanol test, and two samples presented titer 100 and two titer 50. [ABSTRACT FROM AUTHOR]