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In recent years, large quantities of phosphate fertilizers have been applied to crops, including corn, generating high production costs for the producer. One strategy to minimize fertilizer costs and maximize the absorption and release of P from the soil is the use of microorganisms capable of forming associations and solubilizing less labile forms of P in the soil. Therefore, the objective of the present work was to evaluate the development and productivity of corn crops as a function of doses of phosphorus associated with phosphate-solubilizing microorganisms. The experiment was carried out in the UNIVAG experimental field, in the municipality of Várzea Grande – MT, from July to November 2023. An experimental design was used in randomized blocks, in a 5x2 factorial scheme, with five doses of phosphate fertilizer (0, 60, 80, 100, 120 kg/ha) with and without BiomaPhos® inoculation via seed treatment, with three replications, totaling 30 plots. Morphological parameters were evaluated, such as plant height (m), height of insertion of the first ear (cm) and stem diameter (mm), while productive parameters were evaluated: productivity (kg/ha) and seed weight. thousand grains (g). There was no significant effect of the isolated doses and inoculant, as well as the interaction, on the morphological and productive parameters of the corn crop. The addition of phosphorus-solubilizing bacteria (Bacillus subtilis and Bacillus megaterium), regardless of the phosphorus dose tested, did not interfere with the development and productivity of corn under the soil and climatic conditions in which the experiment was carried out. [ABSTRACT FROM AUTHOR]

ABSTRACT: In this study, we analyzed the hypothesis that the combination of Nitrospirillum amazonense strain BR11145 with biological fertilizer prepared using Microgeo® and native microbiome from location of the product application results in morphological and nutritional gains for the initial development of sugarcane plants and soil chemistry. For this purpose, pre-sprouted sugarcane seedlings were grown in a greenhouse mesocosm experiment using soil amended with nitrogen/phosphorus/potassium fertilizer. The experimental treatments consisted of: 1) biological fertilizer with the addition of N. amazonense (100 mL ha-1), 2) biological fertilizer without the addition of N. amazonense, 3) inoculation with N. amazonense at a dose of 100 mL ha-1 with 2x108 viable cell mL-1, 4) inoculation with N. amazonense at a dose of 200 mL ha-1 with 2x108 viable cell mL-1, and 5) control, without the addition of biological fertilizer and N. amazonense. The biological fertilizer was applied at dose of 300 L ha-1, which was split at planting (200 L ha-1) and in the post-emergence phase (100 L ha-1). After 164 days of planting, it was detected an increase in leaf length +3, number of green leaves, leaf area and sulfur content in the leaves of sugarcane plants that received the biological fertilizer with the addition of N. amazonense. In conclusion, the combination of N. amazonense with biological fertilizer revealed positive effects through morphological and nutritional characteristics in sugarcane plants during their early stages of development when compared to plants grown only with the inoculation of N. amazonense, biological fertilizer or mineral fertilizers, with few notable positive effects on soil chemistry.

The aim of this study was to estimate the chemical composition of maize silage based on the morphological characteristics of maize plants and to evaluate the effect of nitrogen fertilization and the inclusion of a microbial inoculant during the ensiling process on the production of maize silage and its morphological, qualitative and fermentative characteristics. The experimental treatments consisted of four levels of nitrogen fertilization with urea (0, 100, 200 and 300 kg ha-1) and the inclusion or exclusion of the microbial inoculants during the ensiling process. A completely randomized design was used in a 4×2 factorial arrangement of treatments. The maize silage chemical composition was estimated by evaluating the plant height (PH) and ear characteristics (NRE = number of rows per ear; NKE = number of kernels per ear; ELS = ear length with straw; EL = ear length without straw) using the following equations: CP = -12.44 + 5.871 × PH + 0.01814 × NRE² (R² = 0.89; P < 0.0001); NDF = 587.93-0.78 × NKE-11.67 × ELS-0.47 × EL + 0.0000007 × NKE³ + 0.006 × EL³ (R² = 0.92; P = 0.003); ADF = 41.48 -0.046 × NRE2 (R2 = 0.42; P = 0.02); TDN = 57.81 - 0.0319 × NRE2 (R2 = 0.42; P = 0.02); EDDM = 56.58 + 0.035 × NRE2 (R2 = 0.42; P = 0.02) and NEL = 1.31 + 0.000757 × NRE2 (R2 = 0.41; P = 0.02). In conclusion, nitrogen fertilization increases the silage energy and protein content; while the inclusion of microbial inoculants during the ensiling process does not alter the chemical and fermentative characteristics of the maize silage.

The aim of this study was to evaluate the fermentative losses, chemical composition and in vitro organic matter digestibility of corn silages produced in different maturity stages microbiologically inoculated. Two commercial inoculants were applied in the silages produced in stages SLL, 1/3 LL, 1/2 LL, 2/3 LL and CN, remaining an uninoculated treatment (control silage), illustrating a factorial scheme 3 x 5. The lactic acid bacteria (LAB) inoculation resulted in lower fermentative losses (P=0.0348), whereas silages produced with dried plants present lower DM losses (P<0.01). Because of inoculant application, there was higher concentrations of CP in the silages produced in stages SLL, 2/3 LL and CN (P=0.0033) and concentration lower of NDF (P=0.0140) in CN stage because of the Maize All® inoculant. This inoculant provided increase in the IVOMD coefficients when the plants were harvested with 2/3 LL and CN (P= 0.0006). Fermentative losses decreased because of the use of microbial inoculants, and this fact it is also observed in silages produced with dried plants. Application of lactic acid bacteria (Maize All® inoculant) in corn silages produced with plants harvested in more advanced stages of maturity improve the chemical composition and in vitro digestibility. [ABSTRACT FROM AUTHOR]

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The research on diazotrophic bacteria in maize has shown the need to associate effective bacteria to promising genotypes, which would benefit from this association. Thus, an experiment was conducted under field conditions in the municipality of Marechal Cândido Rondon-PR, Brazil, aiming at evaluating the performance of two maize hybrids with Herbaspirillum seropedicae inoculation under different nitrogen levels. The study was carried out in experimental design of randomized blocks in factorial 2 x 2 x 3 with five replications. The treatments consisted of two hybrids (AS 1540 and AS 1560); presence or absence of inoculation with H. seropedicae, and three levels of nitrogen in covering (0, 40 and 80 kg ha-1). The data obtained showed that H. seropedicae inoculation did not significantly influenced the productivity of the two tested maize hybrids; however, it is observed that the hybrid AS 1570 responded positively to inoculation with an 8.6% increase in grain production, and hybrid AS 1540 was negatively impacted by a yield reduction of 5.1%, showing that the hybrids tested had different responses to H. seropedicae inoculation. The doses of N applied in covering significantly influenced most of the variables. [ABSTRACT FROM AUTHOR]

Cowpea bean is an important culture for the North and Northeast regions in Brazil for its adaptability to soilclimatic changes and for being one of the main protein sources for these regions population. Therefore, this work aimed to select symbiotic diazotrophic bacteria strains to be used as inoculants for Cowpea bean (Vigna unguiculata (L.) Walp) cultivation, in the Middle North region of the Piauí State, Brazil. The experiment was installed into Middle North EMBRAPA Experimental Station, located in the municipality of Teresina, PI, Brazil. Treatments consisted of four strains inoculations recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) for cultivation of the cowpea bean INPA 03-11b (BR 3301), UFLA 03-84 (BR 3302), BR 3267 and BR 3262, and also the strain BR 3299 to be tested, two witness with nitrogen fertilization addition (50 kg ha-1 during plantation and; 80 kg ha-1, 40 kg during plantation and 40 kg in covering layer 30 days after plantation) and one witness without nitrogen adubation and without inoculation. Recommended strains INPA 03-11b (BR 3301), BR 3267, BR 3262 and BR 3267 and the tested strain BR 3299 responded satisfactorily to agronomic efficiency tests and presented high potential for use as inoculants for cowpea bean in the Middle North region of Piauí. [ABSTRACT FROM AUTHOR]

This experiment aimed to evaluate common beans (Phaseolus vulgaris L.) response to different soil pH levels in the presence and absence of inoculation. Three grams of peat based inoculant with 107 viable cells g-1 of Rhizobium tropici II B strain CIAT 899 were mixed with 5 kg soil samples in plastic bags and incubated for 30 days. Part of the inoculated samples was humidified, another part received CaCO3 and MgO to increase pH to 5.0 and 5.5 and a third part received acetic acid to decrease soil pH to 4.0. Before sowing, seeds were disinfected and (a) part of them was inoculated with 1 mL per seed of liquid culture of R. tropici II B CIAT 899 107 UFC mL-1,while the remainder was not inoculated again. Chemical fertility characteristics were determined at each experimental unit before sowing as well as shoot, root system and nodules dry matters, total N, nodule number and size at 21 days after sowing. Linear correlation analyses were done for soil plant and nodulation characteristics at each harvest time. Low soil pH affects more bean plant in the initial growth stages, while the soil-inoculated rhizobium strain survives, nodule beans and shows effective symbiosis, as indicated by plant N contents. [ABSTRACT FROM AUTHOR]

Selection of peats inoculants with diazotrophic bacteria in two rice varieties. The study consisted of a comparison of two-peat materials (Brazil and Canada) containing different C-total content. The peats were inoculated with strains of Herbaspirillum seropedicae ZAE 94, Burkholderia sp. M130, and Azospirillum brasilense Sp109, and monitored during a period of six months in relation to variation on humidity and survival of bacteria in the inoculant. The quantification of viable cells in the inoculant was measured by the Most Probable Number (MPN) method. The rice seeds were pelleted with the respective inoculants, grown in pots containing soil and maintained outside a greenhouse. The dry mass accumulation, N percent, N-total and yield were determined during the plant cycle. The humidity content varied with storage period. The survival measurement showed that only Burkholderia sp. M130 maintained the number of viable cells around 108 g-1 of peat, while there was a reduction in population of other strains. An increase of yield and total N of 13 and 19.4%, respectively in comparison to treatment fertilized with 40 kg N ha-1 was observed for variety IAC4440, inoculated with strain ZAE94. There was no difference in yield of the IR42 inoculated with either ZAE94 or M130, as compared to N control treatment. No significant difference in development of both rice varieties was observed for both peat used. The results suggest that peat can be used as a carrier for production of an inoculant based on diazotrophic bacteria, since it allowed maintenance of a bacterial population up to 108 cells g-1 peat during the storage period of up to 100 days. The results obtained encourage the practice of inoculation of non-leguminous plants. [ABSTRACT FROM AUTHOR]

Cowpea (Vigna unguiculata (L.) Walp.) is a legume present mainly in tropical regions, capable of biological nitrogen fixation in symbiosis with bacteria known as rhizobia. The action of efficient rhizobial inoculants may be limited by the high competitiveness of the native strains. This study aimed to evaluate the efficiency of inoculation of cowpea cv. Sempre-Verde with native rhizobia obtained from soil samples collected in the Agreste region of Paraíba state. In the first experiment, 52 native rhizobia isolated from the soil samples were tested, together with strain BR 3267 and two treatments with presence and absence of mineral N, in Leonard jars. 13 isolates were selected for a second experiment in pots filled with Regolithic Neosoil, and compared with the recommended strain and the treatments with and without mineral N. The cowpea plants responded to the N addition and to the inoculations. Twelve native isolates promoted aboveground biomass and relative effectiveness that were equivalent to those obtained with the recommended strain or the N fertilization. Aboveground biomass and total N were highly correlated with the relative effectiveness of the isolates. [ABSTRACT FROM AUTHOR]

Copyright of Ciência Animal Brasileira is the property of Universidade Federal de Goias, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)