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Introduction: Fat grafting is an autologous graft of cells from subcutaneous tissue, which can be used as a complementary technique in breast reconstruction. Given this, the cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a way to collect them in one surgical procedure and after performing fractional fat grafting. Dimethyl sulfoxide (DMSO) is a cryopreservative used in cell research, but it is potentially toxic, which would make it impossible to use cryopreserved ADMSCs in clinical practice. New cellular cryopreservatives, without toxicity, have been described in the experimental scientific literature, such as the substances L-proline and trehalose. Therefore, this work aimed to evaluate the viability of ADMSCs cryopreserved with the combination of L-proline and trehalose over up to 90 days. Method: Experimental study in which lipoaspirate samples were obtained from 9 patients. The cellular fraction was processed and frozen with L-proline (1.5M) + trehalose (0.2M) or with DMSO + fetal bovine serum (FBS) as control. After 30 and 90 days, the samples were thawed, and cell viability was assessed using the MTT technique. Results: The analysis of ADMSCs, after 1 and 3 months of freezing, indicated that samples treated with L-proline + trehalose showed similar viability to those preserved with DMSO and SFB (p=0.444). Conclusion: The association of L-proline and trehalose kept ADMSC viable for 30 and 90 days of freezing, and could be an alternative as a cellular cryopreservative without toxicity and enabling the use of serial fat grafting.

Introduction: Lipografting is an alternative with important applicability for breast reconstruction and/or corrections of asymmetries resulting from cancer treatment. This technique consists of autologous fat transfer, whose stroma contains stem cells derived from adipose tissue that can differentiate itself throughout the mesodermal lineage. For adipose tissue preparation, Coleman-based centrifugation of syringe-aspirated material at 3000 revolutions per minute (rpm) for 3 minutes. However, studies question whether lower centrifugation speeds could be less harmful to cell viability. Methods: An experimental study was conducted to evaluate the adipose cells of six patients; from 60mL of liposuction of each one. The sample collected was fractionated into four tubes and submitted to different protocols, decanting and centrifugation at speeds 500, 1000, and 3000rpm for 3 minutes. Afterward, the samples were processed with collagenase IA for 30 min, submitted to cell culture for 24 hours, and a cell viability analysis. The results were tabulated and analyzed by the ANOVA test using the Graphpad Prism 6.0® and SAS®. Results: Cell viability was higher in the cell sample centrifuged at 3000rpm and lower in the decanted sample. Giemsa staining indicated maintenance of cell morphology on the samples. Conclusion: Centrifuged cells at a speed of 3000rpm showed higher cell viability. Centrifugation was effective in compacting tissue and eliminating unwanted waste (blood and residual oil).

Objetivo: determinar a epidemiologia e as características clínicas do melanoma cutâneo (MC) na região Oeste do estado de Santa Catarina, Brasil. Métodos: este estudo transversal e descritivo avaliou o comportamento clínico e o perfil epidemiológico do MC em pacientes de 78 municípios do Oeste do Estado de Santa Catarina, Brasil, no período de 2002 a 2016, tratados no Sistema Único de Saúde. Para a análise dos dados foi utilizado o teste do Qui-quadrado. Para elaborar as curvas de sobrevida, foi considerado um período de 10 anos (teste Log Rank). Resultados: dados de 1.146 pacientes foram avaliados, e houve uma média de 20 casos/100.000 habitantes/ano. Encontravam-se nos estágios I e II 63,5% dos pacientes. A sobrevida global em 10 anos de acompanhamento foi de 89%(IC95% 87,7% - 91,5%). Pacientes do sexo feminino apresentaram maior sobrevida (94,5%; IC95% 92,7% - 96,4% vs. 82,6%; IC95% 78,9% - 86,3%; p < 0,001) e variáveis de melhor prognóstico. Conclusões: o Oeste do estado de Santa Catarina apresentou uma alta prevalência de MC. As características clínicas e epidemiológicas encontradas concordam com as descritas nas populações que residem em regiões geográficas equivalentes; mas diferem de outras regiões do Brasil. Essas diferenças, associadas à organização do sistema público de saúde de referência e contra-referência, podem explicar aos resultados de sobrevida encontrados.