4 results on '"Isolation and purification"'
Search Results
2. Seasonal distribution of gill parasites in fishes from fee-fishing at Guariba, São Paulo State, Brazil
- Author
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Schalch, Sergio H C, Moraes, Flávio R de, and Universidade Estadual Paulista (Unesp)
- Subjects
fish ,Gills ,isolation and purification ,parasitology ,parasite ,Fishes ,Animals ,animal ,gill ,Parasites ,Seasons ,season ,Brazil - Abstract
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:38Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:29:31Z : No. of bitstreams: 1 2-s2.0-33745556562.pdf: 452065 bytes, checksum: bbd40ee55096c24d690b4443f54463ce (MD5) Made available in DSpace on 2014-05-27T11:21:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-10-01 This study was accomplished with the objective of knowing the seasonal distribution of the main species of parasites causes of branchial damages to the fish from fee-fishing at Guariba, State of São Paulo. This city is situated at 21 degrees 15'22'' S, 48 degrees 18'58'' W, in altitude 595 meters. This research was conducted between April, 1997 to March, 1999. From 408 fishes examined, 29.1% of them were infested by the protozoans e metazoans. Leporinus macrocephalus showed the highest parasitism by Trichodina sp., although Piaractus mesopotamicus and Cyprinus carpio were also parasited. Piscinoodinium pillulare infestation were found in L. macrocephalus, P. mesopotamicus, C. carpio e Colossoma macropomum. Monogenetics metazoan have largely been observed in L. macrocephalus, P. mesopotamicus, C. carpio, C. macropomum e cross-bred tambacu. The incidence of this parasite was elevated in the summer for L. macrocephalus, spring for P. mesopotamicus and C. macropomum, and both seasons for cross-bred tambacu. Lernaea cyprinacea copepodes were observed in L. macrocephalus, P. mesopotamicus, C. carpio and cross- breeding tambacu, in spite of the fact that C. carpio has demonstrated high parasitism of adult form. The fish L. macrocephalus, P. mesopotamicus and C. carpio were sponged intensely by Dolops carvalhoi, in situation of high population density. Piaractus mesopotamicus was considered the species more parasited by myxosporídeos, that were also observed in L. macrocephalus and cross-breeding tambacu.
- Published
- 2005
3. Species of the genus Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) in dairy goats maintained in intensive system in the São José do Rio Preto region, São Paulo State, Brazil
- Author
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Freitas, Fagner Luiz da C, Almeida, Katyane de S, Nascimento, Adjair A do, Machado, Célio R, Veschi, Josir L A, Machado, Rosangela Z, and Universidade Estadual Paulista (Unesp)
- Subjects
Male ,animal husbandry ,Goat Diseases ,isolation and purification ,parasitology ,Coccidiosis ,Goats ,goat ,Oocysts ,methodology ,oocyst ,goat disease ,female ,Animals ,animal ,Eimeria ,animal disease ,Brazil - Abstract
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:15Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:10Z : No. of bitstreams: 1 2-s2.0-26944471215.pdf: 510207 bytes, checksum: 8b4f30ab558e6aa98fcc7168f24311ff (MD5) Made available in DSpace on 2014-05-27T11:21:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-01-01 Were examined 58 dairy goats, 41 kids and 17 adults, Saanen and Alpine breeds, males and females, in intensive system, in the São José do Rio Preto region, São Paulo state, for detection and identification of Eimeria spp. oocysts. The 58 feces samples analyzed by the Centrifugal-flotation technique, were positive for, at least, one Eimeria species. The Eimeria species found in this research were: E. ninakohlyakimovae (77,6%), E. jolchijevi (72,4%), E. alijevi (63,8%), E. christenseni (63,8%), E. arloingi (62,1%), E. caprovina (56,9%), E. hirci (50,0%) and E. caprina (48,3%). It follows that the high rate of the positive animals and the high frequency of the Eimeria species among the animals demonstrated that the disease is common in dairy goats, kids and adults, in intensive system.
- Published
- 2005
4. Salmonellas and fecal conforms in animal drinking water
- Author
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Luiz Carlos de Souza, Sebastião Timo laria, Gil Vianna Paim, and Universidade Estadual Paulista (Unesp)
- Subjects
Veterinary medicine ,water ,salmonella ,English Abstract ,bacteriological water contamination ,Contaminação bacteriológica da água ,Rural Health ,Biology ,Feces ,Bacteriological water contamination ,Enterobacteriaceae ,Salmonella ,Contaminação bacteriológica da água/análise ,Salmonella/isolamento ,animal husbandry ,isolation and purification ,Animal ,lcsh:Public aspects of medicine ,microbiology ,Public Health, Environmental and Occupational Health ,health ,lcsh:RA1-1270 ,Water Microbiology ,Brazil ,enterobacteriaceae ,Enterobacteriaceae/isolamento - Abstract
De um total de 402 propriedades rurais do Município de Botucatu, SP (Brasil) com atividades de exploração pecuária, foram sorteadas 60 (15%) pelo processo de amostragem probabilística simples. Nestas 60 propriedades localizaram-se 113 bebedouros que eram os mais utilizados pelos animais para sua dessedentação. Foram colhidas amostras de água desses bebedouros visando a pesquisa de bactérias do gênero Salmonella, a determinação do Número Mais Provável (NMP) de bactérias coliformes fecais e a verificação do pH e da temperatura. Obteve-se isolamento positivo para salmonelas nas amostras de água de 15 (13,27%) bebedouros correspondentes a 12 (20%) das 60 propriedades visitadas, identificando-se os seguintes sorotipos: S. dublin, S. newport, S. madelia, S. IV 43:g, z57: -, S. saphra, S. glostrup, S. IV ochsenzool, S. I 9, 12:i: - e dois novos sorotipos S. IV 41:z52: - e S. IV 50:d:-. Das 113 amostras de água analisadas, 14 (12,39%) apresentaram-se com NMP/100 mL de coliformes fecais acima de 4.000. Não foi constatada associação entre o isolamento positivo de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000. O isolamento de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000 deu-se em temperaturas superiores a 18°C e em pH entre 6,0 e 7,0. Sixty small farms of Botucatu County, S.Paulo, Brazil, given over to cattle breeding were studied. These farms account for 15% of the total of 402 farms that exist in the county. The sample was drawn by simple probabilistic technique. There were found to be one hundred and thirteen drinking places located on the farms. Samples of water were taken from these drinking places and examined for bacteria of the genus Salmonella, for the determination of Most Probable Number (MNP) of fecal coliform bacteria as well as determination of the water's pH. Water temperature was measured before collection. Samples from 15 drinking places (13.3%) were positive for Salmonella. The drinking places belonged to 12 of the sixty farms studied (20%). The following serotypes were identifie d: S. dublin, S. newport, S. madelia, S. IV 43:g,z57:-, S. saphra, S. glostrup, S. IV ochsenzool; S. I9,12:i:-and two new serotypes S. IV 41:z52:- and S. IV 50:d:-. Of the 113 samples studied 14 (12,4%) presented MPN/100ml of fecal coliforms above 4,000. There was no relationship between MPN/100ml of fecal coliforms above 4,000 and positivity for Salmonella. Highest positivity both for Salmonella and MPN/100mL of fecal coliforms over 4,000 occurred at temperatures above 18°C. As regards pH, in both situations the highest positivity occurred between 6.0 and 7.0.
- Published
- 1992
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