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Este trabalho foi realizado de maio a outubro de 2003, na Estação Experimental da Universidade Federal do Rio Grande do Sul (EEA-UFRGS), tendo como objetivo avaliar e comparar a precisão do disco medidor de forragem, do bastão graduado e do medidor de capacitância em predizer a massa de forragem de pastagens de azevém anual. O trabalho constou de dois experimentos: o experimento 1 no qual foram avaliados os três métodos numa situação em que o azevém apresentava-se em fase de estabelecimento num delineamento completamente casualizado, com 32 repetições e três tratamentos; e o experimento 2, no qual foi avaliada a massa de forragem pré e pós-pastejo pelo bastão graduado e pelo disco medidor em potreiros sob lotação rotacionada. O delineamento experimental do experimento 2 foi o de blocos completamente casualizados, com quatro repetições; os tratamentos constaram de duas intensidades de pastejo (baixa e moderada) definidas por ofertas de forragem de 2,5 ou 5,0 vezes o potencial de consumo dos cordeiros que foram utilizados. No experimento 1, o bastão graduado apresentou o melhor coeficiente de determinação com a massa de forragem (r²=0,65). No experimento 2, os melhores coeficientes de determinação foram obtidos quando o pasto, sob lotação rotacionada, foi avaliado com o bastão graduado.

A baixa taxa de regeneração in vitro de alguns cultivares de arroz da subespécie indica limita a utilização de técnicas de cultura de anteras como ferramenta para o desenvolvimento de novos cultivares. A identificação de regiões genômicas associadas à formação de calos e regeneração de plantas, a partir do cultivo de anteras, poderá permitir a transferência do caráter por seleção assistida. Duas populações de retrocruzamento foram utilizadas para o mapeamento genético e estudo destes caracteres, sendo Taipei 309// BRS 7 "Taim"/Taipei 309 (53 genótipos) e BRS 7 "Taim"// BRS 7 "Taim"/Taipei 309 (74 genótipos). Duzentas anteras imaturas de cada genótipo utilizado no estudo foram inoculadas em meio NL líquido e, após 40 dias, os calos formados foram transferidos para o meio MS sólido para regeneração de plantas. Todas as plantas doadoras de anteras foram usadas na extração de DNA genômico e sete primers RAPD foram utilizados na geração de marcadores para a construção dos mapas de ligação dos retrocruzamentos estudados. A taxa de formação de calos variou de 2,27 a 3,36% e a taxa de regeneração de plantas 1,38 a 1,82%, não se diferenciando significativamente. Seis grupos de ligação foram obtidos, três em cada população. Um QTL com LOD 3,10 foi detectado para o caráter formação de calos na população Taipei 309 // BRS 7 "Taim" / Taipei 309. Nenhum QTL foi detectado para a característica regeneração de plantas.

Em aveia (Avena sativa L.), a utilização de técnicas que minimizem os efeitos de ambiente na expressão do fenótipo e que facilitem a identificação de genótipos de superior rendimento de grãos, baixa estatura e tolerantes ao alumínio tóxico são aspectos de fundamental importância para o desenvolvimento de cultivares mais promissores e com adaptabilidade a áreas consideradas marginais. Desta forma, genótipos selecionados pelo método colméia de condução de populações segregantes, em três diferentes cruzamentos, foram submetidos a testes simultâneos para tolerância ao alumínio tóxico (Al+3) e insensibilidade ao ácido giberélico (AG3), objetivando a identificação de constituições genéticas superiores. A técnica utilizada possibilitou a seleção de plantas de alto potencial produtivo, menor estatura (insensíveis ao AG3) e mais tolerantes ao Al+3, podendo ser aplicada com sucesso em programas de melhoramento genético com a cultura da aveia hexaplóide cultivada.

Background and Objectives: The care of the wounds of major burn patients triggers severe painful stimuli. The objective of this study was to assess the safety and efficacy of different drug combinations in anesthesia for balneotherapy., Methods: After approval by the Ethics Commission, 200 procedures of balneotherapy in 87 major burn adult patients were evaluated. Midazolam was used in all cases. The vials of ketamine were numbered and, therefore, at the time of the use, one did not know whether racemic or S(+)ketamine was being used. Each morning it was decided by drawing lots whether fentanyl would be used or not in the procedures of that day. Patients were included in one of four groups: ISO/sf (S(+) isomer without fentanyl), ISO/cf (S(+) isomer with fentanyl), RAC/sf (racemic ketamine without fentanyl), and RAC/cf (racemic ketamine with fentanyl). The initial doses proposed were as follows: midazolam, 0.06 mg.kg-1; ketamine, 1.0 to 1.1 mg.kg-1; and fentanyl, 0.8 (1/4)g.kg1-1; additional doses were administered as needed., Results: Only one patient recalled the pain of balneotherapy. In the group that received S(+)ketamine, the use of fentanyl did not bring additional advantages; however, when associated with racemic ketamine, fentanyl reduced the total dose and the number of ketamine boluses. The extension of body surface burned was the main determinant of the severity of post-procedure pain. Reduced pain severity was the main factor considered by patients when grading their satisfaction with the anesthesia., Conclusions: The four different drug combinations proved to be safe and guaranteed the absence of pain during balneotherapy. Characteristics not directly related to the anesthetics proved to be more important in the incidence of post-procedure pain, which was the main factor considered by major burn patient to define their satisfaction with the anesthesia used.

Objective: To analyze the clinical characteristics and evolution of a group of patients with positive sputum cultures for multidrug-resistant (MDR) Mycobacterium tuberculosis and treated at a referral center in the city of Rio de Janeiro, Brazil., Methods: Based on the positive results in sputum cultures for MDR M. tuberculosis, 50 patients were selected, and their clinical data were obtained from the Brazilian Ministry of Health MDR-TB Database. The frequencies of noncompliance, relapses, failures and previous treatments for TB up to diagnosis of MDR-TB were compiled. The radiological patterns were classified as unilateral or bilateral, and with or without cavitation. Two years after the end of the standard treatment for MDR-TB, the outcome (cure, failure, noncompliance or death) for each patient was evaluated and reassessed every two years. The post-treatment follow-up period was eight years., Results: The mean number of previous treatments was 2.3 +/- 0.9. The mean interval between the initial diagnosis and the development of MDR-TB was 2.0 +/- 1.7 years. Two years after the initial treatment for MDR-TB, 2 patients had abandoned treatment, 8 had died, 18 had been cured, and 22 had presented treatment failure. The bivariate analysis showed that bilateral pulmonary involvement and cavitary pattern markedly reduced the chances for cure, with a relative risk of 1-0.6 (40%) and 1-0.7 (30%), respectively. At the end of the follow-up period, 2 patients had abandoned treatment, 9 had presented treatment failure, 17 had been cured, and 22 had died., Conclusions: Bilateral pulmonary involvement and cavity pattern greatly reduced the chances for cure of the patients with MDR-TB. Most patients who presented treatment failure died within the 8-year follow-up period.

Background and Objectives: Deep analgesia of burned patients needing daily care of their injuries is a field where references are very scarce in the literature. This study aimed at evaluating balneotherapy anesthetic technique in 2852 procedures., Methods: A one-year period prospective evaluation was performed on sedative and analgesic alternatives adopted by anesthesiologists. Age, weight, gender, burned surface area (BSA), drugs and doses used, duration, monitoring, number of sessions by patient, and complications were recorded. Samples was divided in four groups according to age: group A, < 10 years; group B, 11 to 17 years; group C, 18 to 65 years; group D, > 65 years, and the results were compared., Results: There have been 2852 balneotherapy sessions performed in 134 patients: Group A = 743 sessions in 42 patients; Group B = 354 sessions in 16 patients; Group C = 1573 sessions in 68 patients; Group D = 182 sessions in 8 patients. Throughout the study, the intravenous S(+)-ketamine as single agent was used in 116 sessions. The intravenous S(+)-ketamine with midazolam was used in 631 sessions. The intravenous S(+)-ketamine with midazolam and fentanyl was used in 1562 sessions. The muscular S(+)-ketamine as single agent was used in 188 sessions, being 173 in group A. The propofol was associated in 149 sessions. The racemic ketamine was used in 142 sessions. The alfentanil, as some other agents, was seldom used. Sessions lasted 29.3 +/- 10.6 minutes and the increased weight and/or BSA was correlated to significantly longer sessions. There have been 30 cases of SpO2 below 90%, being 3 cases (0.59%) in patients receiving intravenous S(+)-ketamine and midazolam, and 24 cases (1.93%) in patients receiving intravenous S(+)-ketamine, midazolam and fentanyl (p = 0.039). There has been one case of cardiac arrest in moribund patient, who was resuscitated and the session was completed., Conclusions: The S(+)-ketamine, midazolam and fentanyl were the most common agents, being the S(+)-ketamine the major agent. Anesthetic techniques were safe and effective.