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This study aimed to evaluate the possibility of substitution of paraformaldehyde to disinfect nest broiler breeders. Three different products were used, chlorophenol, paraformaldehyde and aluminosilicate associated with organic acids, distributed in the form of spray from the nest. After disinfections procedures, eggs were evaluated for presence of total mesophyles, mould and yeast, embryo diagnosis and analysis of hatchability. Differences were not detected among treatments for microbiological results. Hatchability rates were: 85.71% for paraformaldehyde, 85.28% for chlorophenol and 83.99% for aluminosilicate. Eggs disinfected with paraformaldehyde had higher embryo mortality in the period from 4 to 18 days, suggesting toxicity. The best efficiency results in reduction of microbial contamination and hatchability were observed with the use of chlorophenol. [ABSTRACT FROM AUTHOR]